Patent US5459047A successfully refreshed from Google Patents.
US5459047A
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US
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BMP-6 proteins
Assignee: Genetics Institute LLC
•
Filing: 1994-05-27
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Granted: 1995-10-17
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Expires: 2014-05-27
Abstract
Abstract Purified BMP-6 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.
Inventors
- 👤 John M. Wozney
- 👤 Elizabeth A. Wang
- 👤 Vicki A. Rosen
- 👤 Anthony J. Celeste
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 06:50
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; A; A61; A61L; A61L24/00; A61L24/04; A61L24/043; A61L27/00; A61L27/14; A61L27/22; A61L27/227; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/84
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
40 words
A composition comprising a purified human BMP-6 protein having the amino acid sequence set forth in FIG. 3 or a biologically active fragment thereof wherein said fragment is capable of inducing bone and/or cartilage formation in the Rosen-modified Sampath-Reddi assay.
Claim 2
Dependent Claim
40 words
A composition comprising a purified human BMP-6 protein having the amino acid sequence set forth in FIG. 3 or a biologically active fragment thereof wherein said fragment is capable of inducing bone and/or cartilage formation in the Rosen-modified Sampath-Reddi assay.
Claim 3
Dependent Claim
21 words
The composition of claim 2 wherein said amino acid sequence comprises amino acid #412 to amino acid #513 of FIG. 3.
Claim 4
Dependent Claim
21 words
The composition of claim 2 wherein said amino acid sequence comprises amino acid #388 to amino acid #513 of FIG. 3.
Claim 5
Dependent Claim
21 words
The composition of claim 2 wherein said amino acid sequence comprises amino acid #382 to amino acid #513 of FIG. 3.
Claim 6
Dependent Claim
11 words
The composition of claim 2 further comprising a pharmaceutically acceptable matrix.
Claim 7
Dependent Claim
24 words
The composition of claim 6 wherein said matrix comprises a material selected from the group consisting of hydroxyapatite, collagen, polylactic acid and tricalcium phosphate.
Claim 8
Dependent Claim
79 words
A composition comprising a purified human BMP-6 protein produced by the steps of (a) culturing in a suitable culture medium a cell transformed with a DNA sequence comprising the nucleotide sequence of FIG. 3 or a fragment thereof which encodes a biologically active peptide capable of inducing bone and/or cartilage formation in the Rosen-modified Sampath-Reddi assay; and (b) recovering from said culture medium a protein having the amino acid set forth in FIG. 3 or said biologically active peptide.
Claim 9
Dependent Claim
79 words
A composition comprising a purified human BMP-6 protein produced by the steps of (a) culturing in a suitable culture medium a cell transformed with a DNA sequence comprising the nucleotide sequence of FIG. 3 or a fragment thereof which encodes a biologically active peptide capable of inducing bone and/or cartilage formation in the Rosen-modified Sampath-Reddi assay; and (b) recovering from said culture medium a protein having the amino acid set forth in FIG. 3 or said biologically active peptide.
Claim 10
Dependent Claim
11 words
The composition of claim 8 wherein said host cells arc mammalian.
Claim 11
Dependent Claim
12 words
The composition of claim 8 wherein said host cells are CIIO cells.
Claim 12
Dependent Claim
11 words
A composition of claim 8 further comprising a pharmaceutically acceptable matrix.
Claim 13
Dependent Claim
23 words
The composition of claim 8 wherein said matrix comprises a material from the group consisting of hydroxyapatite, collagen, polylactic acid and tricalcium phosphate.
Claim 14
Dependent Claim
22 words
The composition of claim 8 wherein said protein recovered from the culture medium comprises amino acid #412 to #513 of FIG. 3.
Claim 15
Dependent Claim
21 words
The composition of claim 8 wherein said protein recovered from the medium comprises amino acid #388 to #513 of FIG. 3.
Claim 16
Dependent Claim
21 words
The composition of claim 8 wherein said protein recovered from the medium comprises amino acid #382 to #513 of FIG. 3.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-10-16 | 1999-04-17 | 1999-10-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-10-16 | 2003-04-17 | 2003-10-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-10-16 | 2007-04-17 | 2007-10-17 | $7700.00 | Window Closed / Lapsed |