EP0409472A1
Expired / Lapsed
EP
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Bone morphogenetic protein
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1990-07-11
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Granted: —
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Expires: 2010-07-11
Abstract
Abstract The purification and cloning of bone morphogenetic protein are disclosed, as well as production of BMP and its analogs thereof by recombinant DNA techniques. Pharmaceutical compositions comprising BMP and the use of such compositions are also disclosed.
Inventors
- 👤 Michael C. Keifer
- 👤 Frank R. Masiarz
- 👤 Philip J. Barr
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 13:35
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/22; A; A61; A61P; A61P43/00; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/02; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
18 words
A composition comprising mammalian bone morphogenetic protein (BMP) and analogs thereof substantially free of other osteoinductive associated factors.
Claim 2
Dependent Claim
18 words
A composition comprising mammalian bone morphogenetic protein (BMP) and analogs thereof substantially free of other osteoinductive associated factors.
Claim 3
Dependent Claim
15 words
A composition according to claim 1, wherein said mammalian bone morphogenetic protein is human BMP.
Claim 4
Dependent Claim
15 words
A composition according to claim 1, wherein said mammalian bone morphogenetic protein is bovine BMP.
Claim 5
Dependent Claim
26 words
A composition according to claim 1 or 2, wherein the polypeptide comprises an amino acid sequence aa₁-aa₁₈₂ as shown in Fig. 5 or a fragment thereof.
Claim 6
Dependent Claim
26 words
A composition according to claim 1 or 3, wherein the polypeptide comprises an amino acid sequence aa₁-aa₁₈₀ as shown in Fig. 3 or a fragment thereof.
Claim 7
Dependent Claim
16 words
A composition according to claim 5, wherein said polypeptide comprises of a 16KD yeast cleavage analog.
Claim 8
Dependent Claim
21 words
An intron-free DNA sequence or its complement encoding a polypeptide comprising an amino acid sequence of mammalian BMP and analogs thereof.
Claim 9
Dependent Claim
21 words
An intron-free DNA sequence or its complement encoding a polypeptide comprising an amino acid sequence of mammalian BMP and analogs thereof.
Claim 10
Dependent Claim
13 words
An DNA sequence according to claim 7, wherein said DNA encodes human BMP.
Claim 11
Dependent Claim
13 words
A DNA sequence according to claim 7, wherein said DNA encodes bovine BMP.
Claim 12
Dependent Claim
16 words
A DNA sequence according to claim 7, wherein said polypeptide comprises the mammalian BMP signal sequence.
Claim 13
Dependent Claim
18 words
A DNA sequence according to claim 7, wherein said polypeptide does not comprise the mammalian BMP signal sequence.
Claim 14
Dependent Claim
26 words
A DNA sequence according to claim 7, wherein said polypeptide further comprises an N-terminal yeast alpha-factor signal sequence that provides for secretion in a yeast host.
Claim 15
Dependent Claim
10 words
A replicon comprising a DNA sequence according to claim 8.
Claim 16
Dependent Claim
10 words
A vector comprising a DNA sequence according to claim 8.
Claim 17
Dependent Claim
9 words
A cell comprising a replicon according to claim 13.
Claim 18
Dependent Claim
9 words
A cell comprising a vector according to claim 14.
Claim 19
Independent Claim
76 words
A method of producing recombinant mammalian BMP or an analog thereof comprising: (a) providing a population of cells comprising a heterologous DNA sequence, wherein said DNA sequence comprises (i) transcriptional and translational control sequences functional in said cells, and (ii) a coding sequence under the control of said transcriptional and translational sequences that encodes a polypeptide comprises mammalian BMP and analogs thereof; and (b) growing said population of cells under conditions whereby said polypeptide is expressed.
Claim 20
Independent Claim
76 words
A method of producing recombinant mammalian BMP or an analog thereof comprising: (a) providing a population of cells comprising a heterologous DNA sequence, wherein said DNA sequence comprises (i) transcriptional and translational control sequences functional in said cells, and (ii) a coding sequence under the control of said transcriptional and translational sequences that encodes a polypeptide comprises mammalian BMP and analogs thereof; and (b) growing said population of cells under conditions whereby said polypeptide is expressed.
Claim 21
Dependent Claim
11 words
The method of claim 17, wherein said polypeptide is human BMP.
Claim 22
Dependent Claim
11 words
The method of claim 17, wherein said polypeptide is bovine BMP.
Claim 23
Dependent Claim
11 words
The method claim 17, wherein said population of cells are microorganisms.
Claim 24
Dependent Claim
12 words
The method of claim 17, wherein said population of cells are yeast.
Claim 25
Dependent Claim
14 words
The method of claim 17, wherein said population of cells are E. coli .
Claim 26
Dependent Claim
27 words
The method of claim 18, wherein said polypeptide comprises a signal sequence at the N-terminus of said polypeptide, and said polypeptide is secreted from the said cells.
Claim 27
Dependent Claim
21 words
The method of claim 23, wherein said cells are yeast cell and said signal sequence is a yeast alpha-factor signal sequence.
Claim 28
Independent Claim
28 words
A method for inducing bone growth comprising the step of contacting bone tissue with an effective amount of a composition of mammalian BMP or an active analog thereof.
Claim 29
Independent Claim
28 words
A method for inducing bone growth comprising the step of contacting bone tissue with an effective amount of a composition of mammalian BMP or an active analog thereof.
Claim 30
Dependent Claim
12 words
A monoclonal antibody to a mammalian bone morphogenetic protein or analog thereof.
Claim 31
Dependent Claim
12 words
A monoclonal antibody to a mammalian bone morphogenetic protein or analog thereof.
References Cited (Backward Citations)
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