US5187076A
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US
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DNA sequences encoding BMP-6 proteins
Assignee: Genetics Institute LLC
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Filing: 1990-03-07
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Granted: 1993-02-16
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Expires: 2010-03-07
Abstract
Abstract Purified BMP-6 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.
Inventors
- 👤 John M. Wozney
- 👤 Elizabeth A. Wang
- 👤 Vicki A. Rosen
- 👤 Anthony J. Celeste
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:40
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; A; A61; A61L; A61L24/00; A61L24/04; A61L24/043; A61L27/00; A61L27/14; A61L27/22; A61L27/227; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A61K; A61K38/00
Claims Analysis
Total Claims: 39
Claim 1
Independent Claim
107 words
An isolated DNA sequence encoding a BMP-6 protein said DNA sequence comprising the nucleotide sequence selected from the group consisting of: a) nucleotide #1 through nucleotide #666 of FIG. 2; b) nucleotide #1393 to #1698 of FIG. 3; c) nucleotide #1 to #2393 of FIG. 3; d) nucleotide #160 to #1698 of FIG. 3; e) nucleotide #1303 to #1698 FIG. 3; f) allelic variants and degenerative sequences of (a) through (d); and g) sequences which (1) hybridize to said DNA sequence under stringent hybridization conditions; and (2) encode a protein characterized by the ability to induce the formation of cartilage and/or bone in the Rose-modified Sampath-Reddi assay.
Claim 2
Dependent Claim
107 words
An isolated DNA sequence encoding a BMP-6 protein said DNA sequence comprising the nucleotide sequence selected from the group consisting of: a) nucleotide #1 through nucleotide #666 of FIG. 2; b) nucleotide #1393 to #1698 of FIG. 3; c) nucleotide #1 to #2393 of FIG. 3; d) nucleotide #160 to #1698 of FIG. 3; e) nucleotide #1303 to #1698 FIG. 3; f) allelic variants and degenerative sequences of (a) through (d); and g) sequences which (1) hybridize to said DNA sequence under stringent hybridization conditions; and (2) encode a protein characterized by the ability to induce the formation of cartilage and/or bone in the Rose-modified Sampath-Reddi assay.
Claim 3
Dependent Claim
18 words
A vector comprising a DNA sequence of claim 1 in operative association with an expression control sequence therefor.
Claim 4
Dependent Claim
10 words
A host cell transformed with a vector of claim 2.
Claim 5
Dependent Claim
14 words
A host cell of claim 3 wherein said host cell is a mammalian cell.
Claim 6
Dependent Claim
38 words
An isolated DNA sequence encoding the BMP-6 protein of FIG. 3 or a fragment thereof which encodes a protein characterized by the ability to induce the formation of cartilage and/or bone in the Rosen-modified Sampath-Reddi assay" after "3".
Claim 7
Dependent Claim
38 words
An isolated DNA sequence encoding the BMP-6 protein of FIG. 3 or a fragment thereof which encodes a protein characterized by the ability to induce the formation of cartilage and/or bone in the Rosen-modified Sampath-Reddi assay" after "3".
Claim 8
Dependent Claim
18 words
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #1 to #2923 of FIG. 3.
Claim 9
Dependent Claim
18 words
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #1 to #2923 of FIG. 3.
Claim 10
Dependent Claim
9 words
A vector comprising the nucleotide sequence of claim 6.
Claim 11
Dependent Claim
10 words
A host cell transformed with the vector of claim 7.
Claim 12
Dependent Claim
14 words
The host cell of claim 8 wherein said host cell is a mammalian cell.
Claim 13
Independent Claim
51 words
A method for producing a purified BMP-6 protein said method comprising the steps of (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #1 to #2923 of FIG. 3; and (b) isolating and purifying said protein from said culture medium said BMP-6 protein.
Claim 14
Independent Claim
51 words
A method for producing a purified BMP-6 protein said method comprising the steps of (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #1 to #2923 of FIG. 3; and (b) isolating and purifying said protein from said culture medium said BMP-6 protein.
Claim 15
Dependent Claim
18 words
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #160 to #1698 of FIG. 3.
Claim 16
Dependent Claim
18 words
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #160 to #1698 of FIG. 3.
Claim 17
Dependent Claim
9 words
A vector comprising the nucleotide sequence of claim 11.
Claim 18
Dependent Claim
10 words
A host cell transformed with the vector of claim 12.
Claim 19
Dependent Claim
14 words
The host cell of claim 13 wherein said host cell is a mammalian cell.
Claim 20
Independent Claim
50 words
A method for producing a purified BMP-6 protein said method comprising the steps of (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #5/8to #1698 of FIG. 3; and (b) isolating and purifying said protein from said culture medium said BMP-6 protein.
Claim 21
Independent Claim
50 words
A method for producing a purified BMP-6 protein said method comprising the steps of (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #5/8to #1698 of FIG. 3; and (b) isolating and purifying said protein from said culture medium said BMP-6 protein.
Claim 22
Dependent Claim
18 words
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #1303 to #1698 of FIG. 3.
Claim 23
Dependent Claim
18 words
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #1303 to #1698 of FIG. 3.
Claim 24
Dependent Claim
9 words
A vector comprising the nucleotide sequence of claim 16.
Claim 25
Dependent Claim
10 words
A host cell transformed with the vector of claim 17.
Claim 26
Dependent Claim
14 words
The host cell of claim 18 wherein said host cell is a mammalian cell.
Claim 27
Independent Claim
51 words
A method for producing a purified BMP-6 protein said method comprising the steps of (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #1303 to #1698 of FIG. 3; and (b) isolating and purifying said protein from said culture medium said BMP-6 protein.
Claim 28
Independent Claim
51 words
A method for producing a purified BMP-6 protein said method comprising the steps of (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #1303 to #1698 of FIG. 3; and (b) isolating and purifying said protein from said culture medium said BMP-6 protein.
Claim 29
Dependent Claim
18 words
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #1393 to #1698 of FIG. 3.
Claim 30
Dependent Claim
18 words
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #1393 to #1698 of FIG. 3.
Claim 31
Dependent Claim
9 words
A vector comprising the nucleotide sequence of claim 21.
Claim 32
Dependent Claim
10 words
A host cell transformed with the vector of claim 22.
Claim 33
Dependent Claim
14 words
The host cell of claim 23 wherein said host cell is a mammalian cell.
Claim 34
Independent Claim
61 words
A method for producing a purified BMP-6 protein said method comprising the steps of (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #1393 to #1698 of FIG. 3; and (b) isolating and purifying said protein form said culture medium said protein comprising amino acid #412 to #513 as shown in FIG. 3.
Claim 35
Independent Claim
61 words
A method for producing a purified BMP-6 protein said method comprising the steps of (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #1393 to #1698 of FIG. 3; and (b) isolating and purifying said protein form said culture medium said protein comprising amino acid #412 to #513 as shown in FIG. 3.
Claim 36
Dependent Claim
11 words
An isolated DNA sequence encoding the BMP-6 protein of FIG. 2.
Claim 37
Dependent Claim
11 words
An isolated DNA sequence encoding the BMP-6 protein of FIG. 2.
Claim 38
Dependent Claim
20 words
An isolated DNA sequence encoding a BMP-6 protein comprising the nucleotide sequence from nucleotide #1 to #666 of FIG. 2.
Claim 39
Dependent Claim
20 words
An isolated DNA sequence encoding a BMP-6 protein comprising the nucleotide sequence from nucleotide #1 to #666 of FIG. 2.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-02-16 | 1996-08-17 | 1997-02-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-02-16 | 2000-08-17 | 2001-02-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-02-16 | 2004-08-17 | 2005-02-16 | $7700.00 | Window Closed / Lapsed |