US4627982A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Partially purified bone-inducing factor
Assignee: Celtrix Pharmaceuticals Inc
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Filing: 1985-02-26
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Granted: 1986-12-09
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Expires: 2005-02-26
Abstract
Abstract A partially purified proteinaceous bone-inducing factor of 10,000 to 30,000 daltons is described. It is derived from demineralized bovine bone by extraction with a chaotropic agent, gel filtration, cation exchange chromatography using carboxymethyl cellulose at pH 4.8 and gradient elution with NaCl at 10 mM to about 150 mM.
Inventors
- 👤 Saeid Seyedin
- 👤 Thomas Thomas
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:11
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61K; A61K38/00
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
92 words
A process for preparing a partially purified bone-inducing factor which comprises: (a) treating demineralized bone with a chaotropic extractant that solubilizes nonfibrous proteins; (b) subjecting the extract from step (a) to gel filtration to recover a fraction containing proteins of molecular weight 10,000-30,000; (c) adsorbing the fraction from step (b) onto a carboxymethyl cellulose cation exchanger at approximately pH 4.5-5.5; and (d) eluting an osteogenically active fraction from the cation exchanger with a sodium chloride gradient of about 10 mM to about 150 mM in the presence of a nonionic chaotropic agent.
Claim 2
Dependent Claim
92 words
A process for preparing a partially purified bone-inducing factor which comprises: (a) treating demineralized bone with a chaotropic extractant that solubilizes nonfibrous proteins; (b) subjecting the extract from step (a) to gel filtration to recover a fraction containing proteins of molecular weight 10,000-30,000; (c) adsorbing the fraction from step (b) onto a carboxymethyl cellulose cation exchanger at approximately pH 4.5-5.5; and (d) eluting an osteogenically active fraction from the cation exchanger with a sodium chloride gradient of about 10 mM to about 150 mM in the presence of a nonionic chaotropic agent.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein the extractant is guanidine hydrochloride and the pH is about 4.8.
Claim 4
Dependent Claim
12 words
A partially purified bone-inducing factor prepared by the process of claim 1.
Claim 5
Dependent Claim
12 words
A partially purified bone-inducing factor prepared by the process of claim 2.
Claim 6
Dependent Claim
23 words
An osteogenic implant material comprising: (a) an osteogenically effective amount of a factor of claim 1 reconstituted with (b) a substantially nonimmunogenic carrier.
Claim 7
Dependent Claim
23 words
An osteogenic implant material comprising: (a) an osteogenically effective amount of a factor of claim 2 reconstituted with (b) a substantially nonimmunogenic carrier.
Claim 8
Dependent Claim
24 words
A method of inducing bone formation at a predetermined site in a living mammal comprising implanting the material of claim 5 at said site.
Claim 9
Dependent Claim
24 words
A method of inducing bone formation at a predetermined site in a living mammal comprising implanting the material of claim 6 at said site.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-12-08 | 1990-06-09 | 1990-12-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-12-08 | 1994-06-09 | 1994-12-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-12-08 | 1998-06-09 | 1998-12-09 | $7700.00 | Window Closed / Lapsed |