US4455256A
Expired / Lapsed
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Bone morphogenetic protein
Assignee: University of California
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Filing: 1981-05-05
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Granted: 1984-06-19
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Expires: 2001-05-05
Abstract
Abstract Bone morphogenetic protein (BMP) made by the process comprising the steps of demineralizing bone tissue; treating the demineralized bone tissue under aqueous conditions with a water soluble neutral salt and a solubilizing agent for the BMP, the agent being selected from the group consisting of urea and guanidine, and thereby transforming the bone collagen to gelatin and extracting BMP into the solution of solubilizing agent; and separating the solubilizing agent and neutral salt from the solution, thereby precipitating BMP in the aqueous medium, and the BMP has a molecular weight in the range of 1,000-100,000.
Inventors
- 👤 Marshall R. Urist
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 04:34
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61F; A61F2/00; A61F2/02; A61F2/28; A61K; A61K35/00; A61K35/12; A61K35/32; C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/84; Y10S623/00; Y10S623/915; Y10S623/919
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
98 words
Bone morphogenetic protein (BMP) made by the process comprising the steps of: (a) demineralizing bone tissue; (b) treating said demineralized bone tissue under aqueous conditions with a water soluble neutral salt and a solubilizing agent for said BMP, said agent being selected from the group consisting of urea and guanidine, and thereby transforming the bone collagen to gelatin and extracting BMP into said solution of solubilizing agent; and (c) separating said solubilizing agent and neutral salt from said solution, thereby precipitating BMP in the aqueous medium, and said BMP has a molecular weight in the range of 1,000-100,000.
Claim 2
Dependent Claim
98 words
Bone morphogenetic protein (BMP) made by the process comprising the steps of: (a) demineralizing bone tissue; (b) treating said demineralized bone tissue under aqueous conditions with a water soluble neutral salt and a solubilizing agent for said BMP, said agent being selected from the group consisting of urea and guanidine, and thereby transforming the bone collagen to gelatin and extracting BMP into said solution of solubilizing agent; and (c) separating said solubilizing agent and neutral salt from said solution, thereby precipitating BMP in the aqueous medium, and said BMP has a molecular weight in the range of 1,000-100,000.
Claim 3
Dependent Claim
30 words
The BMP of claim 1, where in said separating step said solubilizing agent and neutral salt are separated from said solution by dialysis thereby precipitating BMP in the aqueous medium.
Claim 4
Dependent Claim
35 words
The BMP of claim 2, including the subsequent step of adding to said BMP precipitate, a solubilizing agent for said BMP precipitate selected from the group consisting of urea and guanidine thereby solubilizing said BMP.
Claim 5
Dependent Claim
28 words
The BMP of claim 3, including the step of washing said BMP precipitate from said dialysis step with citric acid prior to said addition of a solubilizing agent.
Claim 6
Dependent Claim
21 words
The BMP of claim 3, including the subsequent step of coprecipitating BMP and a calcium salt from said solution of BMP.
Claim 7
Dependent Claim
14 words
The BMP of claim 2, wherein said neutral salt is a physiologically acceptable salt.
Claim 8
Dependent Claim
19 words
The BMP of claim 2, wherein said solubilizing agent is urea, and said neutral salt is a chloride salt.
Claim 9
Dependent Claim
12 words
The BMP of claim 7, wherein said neutral salt is calcium chloride.
Claim 10
Dependent Claim
27 words
The BMP of claim 7, wherein said neutral salt is selected from the group consisting of calcium chloride, sodium chloride, potassium chloride, lithium chloride and strontium chloride.
Claim 11
Dependent Claim
13 words
The BMP of claim 3, wherein said subsequently added solubilizing agent is urea.
Claim 12
Dependent Claim
14 words
The BMP of claim 4, including the step of gel filtering said solubilized BMP.
Claim 13
Dependent Claim
26 words
The BMP of claim 11, including the step of recovering a BMP fraction having a molecular weight in the range of between about 1,000 and 100,000.
Claim 14
Dependent Claim
21 words
The BMP of claim 12, wherein said solubilizing agents are urea, and said neutral salt is a physiologically acceptable chloride salt.
Claim 15
Dependent Claim
12 words
The BMP of claim 5, wherein said calcium salt is calcium phosphate.
Claim 16
Dependent Claim
18 words
The BMP of claim 5, wherein each BMP precipitation step is carried out in a low temperature environment.
Claim 17
Dependent Claim
17 words
The BMP of claim 8, wherein said calcium chloride is in a molar solution of about 0.5M.
Claim 18
Dependent Claim
16 words
The BMP of claim 10, wherein said urea is in a molar solution of about 6M.
Claim 19
Dependent Claim
21 words
The BMP of claim 3, wherein said BMP has a molecular weight in the range of between about 20,000 and 63,000.
Claim 20
Dependent Claim
21 words
The BMP of claim 3, wherein said BMP has a molecular weight in the range of between about 10,000 and 63,000.
Claim 21
Dependent Claim
21 words
The BMP of claim 3, wherein said BMP has a molecular weight in the range of between about 20,000 and 50,000.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-06-19 | 1987-12-19 | 1988-06-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-06-19 | 1991-12-19 | 1992-06-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-06-19 | 1995-12-19 | 1996-06-19 | $7700.00 | Window Closed / Lapsed |