CA2017466A1
Expired / Lapsed
CA
⏱️ 0.0 years remaining
Bone calcification factor
Assignee: Novartis Vaccines and Diagnostics Inc
•
Filing: 1990-05-24
•
Granted: —
•
Expires: 2010-05-24
Abstract
Abstract ABSTRACT The isolation, identification and production by recombinant methods of bone calcification factor, a 22KD polypeptide, are disclosed. The peptide has calcification-inducing activity when implanted with matrix Gla protein into mammals.
Inventors
- 👤 Michael C. Kiefer
- 👤 Frank R. Masiarz
- 👤 Philip J. Barr
Bibliographic Details
Application Number
—
Jurisdiction
CA
Data Source
google_patents
Last Synchronized
2026-10-23 11:41
CPC Classifications
—
Claims Analysis
Total Claims: 47
Claim 1
Independent Claim
25 words
A composition comprising a polypeptide selected from the group consisting of mammalian bone calcification factor and analogs thereof substantially free of other osteoinductive associated factors.
Claim 2
Dependent Claim
25 words
A composition comprising a polypeptide selected from the group consisting of mammalian bone calcification factor and analogs thereof substantially free of other osteoinductive associated factors.
Claim 3
Dependent Claim
15 words
A composition according to Claim 1, wherein said mammalian bone calcification factor is human BCF.
Claim 4
Dependent Claim
15 words
A composition according to Claim 1, wherein said mammalian bone calcification factor is bovine BCF.
Claim 5
Dependent Claim
22 words
A composition according to Claim 2 wherein said polypeptide comprises an amino acid sequence as shown in FIG 1A or fragment thereof.
Claim 6
Dependent Claim
23 words
A composition according to Claim 3 wherein said polypeptide comprises an amino acid sequence as shown in FIG. 1B or a fragment thereof.
Claim 7
Dependent Claim
49 words
A composition according to Claim 4, wherein said polypeptide comprises an amino terminus selected from the group consisting of the signal peptide sequence met-asp-lou-ser-leu-leu-trp-val-leu-leu-pro-leu-vaal-thr-mst-ala-trp-gly and sequences of said signal peptide sequence derived by deletion of one or more amino acids from the amino terminus of said signal peptide sequence.
Claim 8
Dependent Claim
49 words
A composition according to Claim 5, wherein said polypeptide comprises an amino terminus selected from the group consisting of the signal peptide sequence met-asp-leu-thr-leu-leu-trp-val-leu-leu-pro-leu-vaal-thr-val-ala-trp-gly and sequences of said signal peptide sequence derived by removal of one or more amino acids from the amino terminus of said signal peptide sequence.
Claim 9
Dependent Claim
7 words
Non-chromosomal DNA as shown in FIG 1C.
Claim 10
Dependent Claim
7 words
Non-chromosomal DNA as shown in FIG 1C.
Claim 11
Dependent Claim
9 words
DNA complementary to the sequence shown in FIG. 1C.
Claim 12
Dependent Claim
9 words
DNA complementary to the sequence shown in FIG. 1C.
Claim 13
Dependent Claim
7 words
Non-chromosomal DNA as shown in FIG 1D.
Claim 14
Dependent Claim
7 words
Non-chromosomal DNA as shown in FIG 1D.
Claim 15
Dependent Claim
9 words
DNA complementary to the sequence shown in FIG. 1D.
Claim 16
Dependent Claim
9 words
DNA complementary to the sequence shown in FIG. 1D.
Claim 17
Dependent Claim
55 words
Non-chromosomal DNA according to Claim 8 further comprising the 5' sequence selected from the group consisting of the leader sequence ATG GAC CTC AGT CTT CTC TGG GTA CTT CTG CCC CTA GTC ACC ATG GCC TGG GGC and sequences derived by removal of one or more deoxynucleotides from the 5'-terminus of said leader sequence.
Claim 18
Dependent Claim
55 words
Non-chromosomal DNA according to Claim 10 further comprising the 5' sequence selected from the group consisting of the leader sequence ATG GAC CTC ACT CTT CTG TGG GTG CTT CTG CCA CTG GTC ACC GTG GCT TGG GGA and sequences derived by removal of one or more deoxynucleotides from the 5'-terminus of said leader sequence.
Claim 19
Independent Claim
52 words
A method comprising the steps of (a) constructing a vector which includes the DNA sequence shown in FIG. 1C or a fragment thereof, (b) transforming a host cell with said vector, and (c) culturing the resultant transformed cell under conditions to express the peptide encoded by said DNA sequence or fragment thereof.
Claim 20
Independent Claim
52 words
A method comprising the steps of (a) constructing a vector which includes the DNA sequence shown in FIG. 1C or a fragment thereof, (b) transforming a host cell with said vector, and (c) culturing the resultant transformed cell under conditions to express the peptide encoded by said DNA sequence or fragment thereof.
Claim 21
Independent Claim
53 words
A method comprising the steps of (a) constructing a vector which includes the DNA sequence shown in FIG. 1D or a calcification-initiating fragment thereof, (b) transforming a host cell with said vector, and (c) culturing the resultant transformed cell under conditions to express the peptide encoded by said DNA sequence or fragment thereof.
Claim 22
Independent Claim
53 words
A method comprising the steps of (a) constructing a vector which includes the DNA sequence shown in FIG. 1D or a calcification-initiating fragment thereof, (b) transforming a host cell with said vector, and (c) culturing the resultant transformed cell under conditions to express the peptide encoded by said DNA sequence or fragment thereof.
Claim 23
Dependent Claim
60 words
A method according to Claim 14 wherein said DNA sequence further comprises a 5' sequence selected from the group consisting of the leader sequence ATG GAC CTC AGT CTT CTC TGG GTA CTT CTG CCC CTA GTC ACC ATG GCC TGG GGC and any sequence derived by removal of one or more deoxynucleotides from the 5'-terminus of said leader sequence.
Claim 24
Dependent Claim
61 words
A method according to Claim 15 wherein said DNA nucleotide sequence further comprises a 5' sequence selected from the group consisting of the leader sequence ATG GAC CTC ACT CTT CTG TGG GTG CTT CTG CCA CTG GTC ACC GTG GCT TGG GGA and any sequence derived by removal of one or more deoxynucleotides from the 5'-terminus of said leader sequence.
Claim 25
Independent Claim
16 words
A method according to any of Claims 14 through 17 wherein said host cell is eukaryotic.
Claim 26
Dependent Claim
11 words
A method according to Claim 18 wherein said host comprises yeast.
Claim 27
Dependent Claim
19 words
A method according to Claim 18 wherein said vector comprises a GAPDH promoter which controls expression of said peptide.
Claim 28
Dependent Claim
19 words
A method according to Claim 18 wherein said promoter comprises an ADH2/GAPDH promoter which controls expression of said peptide.
Claim 29
Dependent Claim
9 words
A replicable vector comprising DNA according to Claim 8.
Claim 30
Dependent Claim
9 words
A replicable vector comprising DNA according to Claim 10.
Claim 31
Dependent Claim
9 words
A replicable vector comprising DNA according to Claim 12.
Claim 32
Dependent Claim
9 words
A replicable vector comprising DNA according to Claim 13.
Claim 33
Dependent Claim
12 words
A host cell transformed with a replicable vector according to Claim 22.
Claim 34
Dependent Claim
12 words
A host cell transformed with a replicable vector according to Claim 23.
Claim 35
Dependent Claim
12 words
A host cell transformed with a replicable vector according to Claim 24.
Claim 36
Dependent Claim
12 words
A hot cell transformed with a replicable vector according to Claim 25.
Claim 37
Dependent Claim
26 words
A composition for inducing calcification, comprising an effective calcification-inducing amount of a mixture of mammalian bone calcification factor or an analog thereof, and matrix Gla protein.
Claim 38
Dependent Claim
26 words
A composition for inducing calcification, comprising an effective calcification-inducing amount of a mixture of mammalian bone calcification factor or an analog thereof, and matrix Gla protein.
Claim 39
Dependent Claim
14 words
A composition according to Claim 30 wherein said factor comprises human bone calcification factor.
Claim 40
Dependent Claim
14 words
A composition according to Claim 30 wherein said factor comprises bovine bone calcification factor.
Claim 41
Dependent Claim
23 words
A composition according to Claim 31 wherein said factor has an amino acid sequence as shown in FIG. 1A, or a fragment thereof.
Claim 42
Dependent Claim
23 words
A composition according to Claim 32 wherein said factor has an amino acid sequence as shown in FIG. 1B, or a fragment thereof.
Claim 43
Independent Claim
42 words
A method for inducing calcification for the formation of bone in a vertebrate, comprising the step of administering to said vertebrae in a pharmaceutically effective manner an effective calcification-inducing amount of a composition according to any one of Claims 3- to 34.
Claim 44
Dependent Claim
30 words
A diagnostic method comprising the steps of providing a bone sample from a mammal and measuring the amount of mammalian bone calcification factor in said sample in a quantitative assay.
Claim 45
Dependent Claim
30 words
A diagnostic method comprising the steps of providing a bone sample from a mammal and measuring the amount of mammalian bone calcification factor in said sample in a quantitative assay.
Claim 46
Dependent Claim
13 words
A composition comprising antibodies recognizing an epitope unique to mammalian bone calcification factor.
Claim 47
Dependent Claim
13 words
A composition comprising antibodies recognizing an epitope unique to mammalian bone calcification factor.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.