US4886747A
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Nucleic acid encoding TGF-β and its uses
Assignee: Genentech Inc
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Filing: 1987-03-13
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Granted: 1989-12-12
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Expires: 2007-03-13
Abstract
Abstract Nucleic acid encoding TGF-β has been isolated and cloned into vectors which are replicated in bacteria and expressed in eukaryotic cells. TFG-β is recovered from transformed cultures for use in known therapeutic modalities. Nucleic acid encoding TGF-β is useful in diagnosis and identification of TGF-β clones.
Inventors
- 👤 Rik M. A. Derynck
- 👤 David V. Goeddel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 02:52
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/495
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
38 words
A method comprising (a) constructing a vector which includes nucleic acid encoding biologically active TGF-β, (b) transforming a host eukaryotic cell with the vector, (c) culturing the transformed cell and (d) recovering mature TGF-β from the culture medium.
Claim 2
Independent Claim
38 words
A method comprising (a) constructing a vector which includes nucleic acid encoding biologically active TGF-β, (b) transforming a host eukaryotic cell with the vector, (c) culturing the transformed cell and (d) recovering mature TGF-β from the culture medium.
Claim 3
Dependent Claim
16 words
The method of claim 1 wherein the eukaryotic cell is a Chinese hamster ovary cell line.
Claim 4
Dependent Claim
18 words
The method of claim 1 wherein the TGF-β encoded by the nucleic acid of step (a) is preTGF-β.
Claim 5
Dependent Claim
30 words
The method of claim 3 wherein the preTGF-β is a fusion polypeptide hving a viral secretory signal sequence at its N-terminus and the sequence of mature TGF-β at its C-terminus.
Claim 6
Dependent Claim
19 words
The method of claim 4 wherein the nucleic acid encoding the preTGF-β is operably linked to a viral promoter.
Claim 7
Dependent Claim
19 words
The method of claim 4 wherein the nucleic acid encoding the preTGF-β is operably linked to an inducible promoter.
Claim 8
Dependent Claim
11 words
The method of claim 1 wherein the TGF-β is human TGF-β.
Claim 9
Independent Claim
38 words
A method comprising (a) constructing a vector that includes nucleic acid encoding biologically active TGF-β1, (b) transforming a host eukaryotic cell with the vector, (c) culturing the transformed cell, and (d) recovering mature TGF-β1 from the culture medium.
Claim 10
Independent Claim
38 words
A method comprising (a) constructing a vector that includes nucleic acid encoding biologically active TGF-β1, (b) transforming a host eukaryotic cell with the vector, (c) culturing the transformed cell, and (d) recovering mature TGF-β1 from the culture medium.
Claim 11
Dependent Claim
16 words
The method of claim 8 wherein the eukaryotic cell is a Chinese hamster ovary cell line.
Claim 12
Dependent Claim
18 words
The method of claim 8 wherein the TGF-β1 encoded by the nucleic acid of step (a) is preTGF-β1.
Claim 13
Dependent Claim
30 words
The method of claim 10 wherein the preTGF-β1 is a fusion polypeptide having a viral secretory signal sequence at its N-terminus and the sequence of mature TGF-β1 at its C-terminus.
Claim 14
Dependent Claim
19 words
The method of claim 11 wherein the nucleic acid encoding the preTGF-β1 is operably linked to a viral promoter.
Claim 15
Dependent Claim
19 words
The method of claim 11 wherein the nucleic acid encoding the preTGF-β1 is operably linked to an inducible promoter.
Claim 16
Dependent Claim
11 words
The method of claim 8 wherein the TGF-β1 is human TGF-β1.
Claim 17
Independent Claim
38 words
A method comprising (a) constructing a vector that includes nucleic acid encoding biologically active TGF-β3, (b) transforming a host eukaryotic cell with the vector, (c) culturing the transformed cell, and (d) recovering mature TGF-β3 from the culture medium.
Claim 18
Independent Claim
38 words
A method comprising (a) constructing a vector that includes nucleic acid encoding biologically active TGF-β3, (b) transforming a host eukaryotic cell with the vector, (c) culturing the transformed cell, and (d) recovering mature TGF-β3 from the culture medium.
Claim 19
Dependent Claim
16 words
The method of claim 15 wherein the eukaryotic cell is a Chinese hamster ovary cell line.
Claim 20
Dependent Claim
18 words
The method of claim 15 wherein the TGF-β3 encoded by the nucleic acid of step (a) is preTGF-β3.
Claim 21
Dependent Claim
30 words
The method of claim 17 wherein the preTGF-β3 is a fusion polypeptide having a viral secretory signal sequence at its N-terminus and the sequence of mature TGF-β3 at its C-terminus.
Claim 22
Dependent Claim
19 words
The method of claim 18 wherein the nucleic acid encoding the preTGF-β3 is operably linked to a viral promoter.
Claim 23
Dependent Claim
19 words
The method of claim 18 wherein the nucleic acid encoding the preTGF-β3 is operably linked to an inducible promoter.
Claim 24
Dependent Claim
11 words
The method of claim 15 wherein the TGF-β3 is human TGF-β3.
Claim 25
Dependent Claim
21 words
A replicable eukaryotic expression vector comprising DNA that encodes biologically active TGF-β operably linked to DNA that encodes a secretory leader.
Claim 26
Dependent Claim
21 words
A replicable eukaryotic expression vector comprising DNA that encodes biologically active TGF-β operably linked to DNA that encodes a secretory leader.
Claim 27
Dependent Claim
10 words
A eukaryotic host cell containing the vector of claim 22.
Claim 28
Dependent Claim
21 words
A replicable eukaryotic expression vector comprising DNA that encodes biologically active TGF-β1 operably linked to DNA that encodes a secretory leader.
Claim 29
Dependent Claim
21 words
A replicable eukaryotic expression vector comprising DNA that encodes biologically active TGF-β1 operably linked to DNA that encodes a secretory leader.
Claim 30
Dependent Claim
10 words
A eukaryotic host cell containing the vector of claim 24.
Claim 31
Dependent Claim
21 words
A replicable eukaryotic expression vector comprising DNA that encodes biologically active TGF-β3 operably linked to DNA that encodes a secretory leader.
Claim 32
Dependent Claim
21 words
A replicable eukaryotic expression vector comprising DNA that encodes biologically active TGF-β3 operably linked to DNA that encodes a secretory leader.
Claim 33
Dependent Claim
10 words
A eukaryotic host cell containing the vector of claim 26.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-12-11 | 1993-06-12 | 1993-12-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-12-11 | 1997-06-12 | 1997-12-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-12-11 | 2001-06-12 | 2001-12-12 | $7700.00 | Window Closed / Lapsed |