US5459047A
Expired / Lapsed
US
⏱️ 0.0 years remaining
BMP-6 proteins
Assignee: Genetics Institute LLC
•
Filing: 1994-05-27
•
Granted: 1995-10-17
•
Expires: 2014-05-27
Abstract
Abstract Purified BMP-6 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.
Inventors
- 👤 John M. Wozney
- 👤 Elizabeth A. Wang
- 👤 Vicki A. Rosen
- 👤 Anthony J. Celeste
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 05:03
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; A; A61; A61L; A61L24/00; A61L24/04; A61L24/043; A61L27/00; A61L27/14; A61L27/22; A61L27/227; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/84
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
40 words
A composition comprising a purified human BMP-6 protein having the amino acid sequence set forth in FIG. 3 or a biologically active fragment thereof wherein said fragment is capable of inducing bone and/or cartilage formation in the Rosen-modified Sampath-Reddi assay.
Claim 2
Dependent Claim
40 words
A composition comprising a purified human BMP-6 protein having the amino acid sequence set forth in FIG. 3 or a biologically active fragment thereof wherein said fragment is capable of inducing bone and/or cartilage formation in the Rosen-modified Sampath-Reddi assay.
Claim 3
Dependent Claim
21 words
The composition of claim 2 wherein said amino acid sequence comprises amino acid #412 to amino acid #513 of FIG. 3.
Claim 4
Dependent Claim
21 words
The composition of claim 2 wherein said amino acid sequence comprises amino acid #388 to amino acid #513 of FIG. 3.
Claim 5
Dependent Claim
21 words
The composition of claim 2 wherein said amino acid sequence comprises amino acid #382 to amino acid #513 of FIG. 3.
Claim 6
Dependent Claim
11 words
The composition of claim 2 further comprising a pharmaceutically acceptable matrix.
Claim 7
Dependent Claim
24 words
The composition of claim 6 wherein said matrix comprises a material selected from the group consisting of hydroxyapatite, collagen, polylactic acid and tricalcium phosphate.
Claim 8
Dependent Claim
79 words
A composition comprising a purified human BMP-6 protein produced by the steps of (a) culturing in a suitable culture medium a cell transformed with a DNA sequence comprising the nucleotide sequence of FIG. 3 or a fragment thereof which encodes a biologically active peptide capable of inducing bone and/or cartilage formation in the Rosen-modified Sampath-Reddi assay; and (b) recovering from said culture medium a protein having the amino acid set forth in FIG. 3 or said biologically active peptide.
Claim 9
Dependent Claim
79 words
A composition comprising a purified human BMP-6 protein produced by the steps of (a) culturing in a suitable culture medium a cell transformed with a DNA sequence comprising the nucleotide sequence of FIG. 3 or a fragment thereof which encodes a biologically active peptide capable of inducing bone and/or cartilage formation in the Rosen-modified Sampath-Reddi assay; and (b) recovering from said culture medium a protein having the amino acid set forth in FIG. 3 or said biologically active peptide.
Claim 10
Dependent Claim
11 words
The composition of claim 8 wherein said host cells arc mammalian.
Claim 11
Dependent Claim
12 words
The composition of claim 8 wherein said host cells are CIIO cells.
Claim 12
Dependent Claim
11 words
A composition of claim 8 further comprising a pharmaceutically acceptable matrix.
Claim 13
Dependent Claim
23 words
The composition of claim 8 wherein said matrix comprises a material from the group consisting of hydroxyapatite, collagen, polylactic acid and tricalcium phosphate.
Claim 14
Dependent Claim
22 words
The composition of claim 8 wherein said protein recovered from the culture medium comprises amino acid #412 to #513 of FIG. 3.
Claim 15
Dependent Claim
21 words
The composition of claim 8 wherein said protein recovered from the medium comprises amino acid #388 to #513 of FIG. 3.
Claim 16
Dependent Claim
21 words
The composition of claim 8 wherein said protein recovered from the medium comprises amino acid #382 to #513 of FIG. 3.
References Cited (Backward Citations)
US4761471A
Cited
US4294753A
Cited
US4789732A
Cited
US4455256A
Cited
US4434094A
Cited
US4804744A
Cited
US4608199A
Cited
US4774322A
Cited
US4774228A
Cited
US4627982A
Cited
US4810691A
Cited
US4843063A
Cited
US4563350A
Cited
US4886747A
Cited
US4681763A
Cited
US4798885A
Cited
US4737578A
Cited
US5187076A
Cited
EP0336760A2
Cited
US4968590A
Cited
WO1989009788A2
Cited
WO1989009787A2
Cited
US5108753A
Cited
US5011691A
Cited
US5106626A
Cited
WO1990003733A1
Cited
CA2017466A1
Cited
EP0409472A1
Cited
WO1991002744A1
Cited
EP0416578A2
Cited
WO1991005802A1
Cited
WO1991018047A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-10-16 | 1999-04-17 | 1999-10-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-10-16 | 2003-04-17 | 2003-10-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-10-16 | 2007-04-17 | 2007-10-17 | $7700.00 | Window Closed / Lapsed |