Osteogenic proteins

Claim 2

A protein, produced by expression of recombinant DNA in a host cell and isolated from said host cell, comprising a pair of polypeptide chains disulfide bonded to form a dimeric species, each of said pair of polypeptide chains having less than 200 amino acids in a sequence sufficiently duplicative of the sequence of: ##STR24## such that said dimeric species has a conformation capable of inducing bone and cartilage formation when implanted in a mammal in association with a matrix.

Claim 3

The osteogenic protein of claim 1 having a molecular weight of about 30 kD when oxidized as determined by comparison to molecular weight standards in SDS-polyacrylamide gel.

Claim 4

The osteogenic protein of claim 2 further characterized by being glycosylated.

Claim 5

The osteogenic protein of claim 1 having a molecular weight of about 27 kD as determined by comparison to molecular weight standards in SDS-polyacrylamide gel electrophoresis.

Claim 6

The protein of claim 1 or 4 further characterized by being unglycosylated.

Claim 7

The protein of claim 1 comprising the amino acid sequences: ##STR25## wherein each X independently represents an amino acid.

Claim 8

The protein of claim 1 comprising the amino acid sequences: ##STR26## wherein each X independently represents an amino acid.

Claim 9

The protein of claim 1 comprising the amino acid sequence: ##STR27##

Claim 10

The protein of claim 1 comprising the amino acid sequences: ##STR28##

Claim 11

The protein of claim 1 comprising the product of expression of a DNA in a procaryotic host cell.

Claim 12

The protein of claim 1 produced by expression in a mammalian cell line.

Claim 13

The protein of claim 1 having a half-maximum bone forming activity of at least about 20-25 ng per 25 mg of implant.

Claim 14

The protein of claim 1 comprising the amino acid sequences: ##STR29## wherein X 1 =(K or R); X 2 =(H,R, or K); X 3 =(P, S, E or Q); X 4 =(Y, K or F); X 5 =(D,S or E); X 6 =(R, S, K or A); X 7 =(V, L, or I); X 8 =(N, Q, D or S); X 9 =(D, E or N); X 10 =(I or V); X 11 =(I or V); X 12 =(A or S); X 13 =(P, E, L or K); X 14 =(Y or F); X 15 =(H or D); X 16 =(F, Y or N); X 17 =(H, E or S); X 18 =(E or A); X 19 =(P, A or Q); X 20 =(F or Y); X 21 =(L, M or I); X 22 =(A, P or T); X 22 =(D, E or K); X 24 =(H or S); X 25 =(L, M or F); X 26 =(N or K); X 27 =(S, A or P); X 28 =(T or S); X 29 =(I, V or T); X 30 =(S, A, F or N); X 35 =(V or I); X 36 =(P or S); X 37 =(G or E); X 38 =(K, Q, T or S); X 39 =(I or V); X 40 =(K or E); X 41 =(A, P or S); X 42 =(V or A); X 43 =(T or E); X 44 =(E, Q or K); X 45 =(L or M); X 46 =(S N or D); X 47 =(A, S or P); X 48 =(I, L or V); X 49 =(S or A); X 50 =(M, I or V); X 51 =(Y or F); X 52 =(L, F or Y); X 53 =(D or N); X 54 =(E, D or N); X 55 =(N or Q); X 56 =(E, D, S or K); X 57 =(N or K); X 58 =(V or I); X 59 =(K or R); X 60 =(N, K or H); X 61 =(Q, E, R or P); X 62 =(D, E or N); X 63 =(V or T); X 64 =(E, D or R); X 65 =(G, A, S or E); X 66 =(G or H); and X 67 =(R or H).

Claim 15

The protein of claim 1 comprising the amino acid sequences: ##STR30## wherein X 1 =(Y, K or F); X 2 =(D, S or E); X 3 =(R, S, K or A); X 4 =(V, L, or I); X 5 =(N, Q, D or S); X 6 =(D, E or N); X 7 =(I or V); X 8 =(I or V); X 9 =(A or S); X 10 =(P, E, L or K); X 11 =(Y or F); X 12 =(H or D); X 13 =(F, Y or N); X 14 =(H, E or S); S 15 =(E or A); X 16 =(P, A or Q); X 17 =(F or Y); X 18 =(L, M or I); X 19 =(A, P or T); X 20 =(D, E or K); X 21 =(H or S); X 22 =(L, M or F); X 23 =(N or K); X 24 =(S, A or P); X 25 =(T or S); X 26 =(I, V, or T); X 27 =(V, I or L); X 28 =(T or S); X 29 =(L or I); X 30 = (N, H or R); X 31 =(S, A, F or N); X 32 =(V or I); X 33 =(P or S); X 34 =(G or E); X 35 =(K, Q, T or S); X 36 =(I or V); X 37 =(K or E); X 38 =(A, P or S); X 39 =(V or A); X 40 =(T or E); X 41 =(E, Q or K); X 42 =(L or M); X 43 =(S N or D); X 44 =(A, S or P); X 45 =(I, L or V); X 46 =(S or A); X 47 =(M, I or V); X 48 =(Y or F); X 49 =(L, F or Y); X 50 =(D or N); X 51 =(E, D or N); X 52 =(N or Q); X 53 =(E, D, S or K); X 54 =(N or K); X 55 =(V or I); X 56 =(K or R); X 57 =(N, K or H); X 58 =(Q, E, R or P); X 59 =(D, E or N); X 60 =(V or T); X 61 =(E, D or R); X 62 =(G, A, S or E); X 63 =(G or H); and X 64 =(R or H).

Claim 16

The protein of claim 1 having a half maximum bone-forming activity of at least 20-25 ng per 25 mg of matrix.

Claim 17

An isolated protein comprising a pair of polypeptide chains disulfide bonded to form a dimeric species, at least one of said polypeptide chains comprising the amino acid sequence: ##STR31##

Claim 18

An isolated protein comprising a pair of polypeptide chains disulfide bonded to form a dimeric species, at least one of said polypeptide chains comprising the amino acid sequence: ##STR31##

Claim 19

An isolated protein comprising a pair of polypeptide chains disulfide bonded to form a dimeric species, at least one of said polypeptide chains comprising the amino acid sequence: ##STR32##

Claim 20

An isolated protein comprising a pair of polypeptide chains disulfide bonded to form a dimeric species, at least one of said polypeptide chains comprising the amino acid sequence: ##STR32##