US5116738A
Expired / Lapsed
US
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DNA sequences encoding
Assignee: Genetics Institute LLC
•
Filing: 1991-04-26
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Granted: 1992-05-26
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Expires: 2011-04-26
Abstract
Abstract Purified BMP-3 proteins and processes for producing them are disclosed. They may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.
Inventors
- 👤 Elizabeth A. Wang
- 👤 John M. Wozney
- 👤 Vicki Rosen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 04:58
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A; A61; A61L; A61L24/00; A61L24/04; A61L24/043; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61K; A61K38/00
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
96 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising a coding sequence selected from the group consisting of (a) nucleostide #414 through nucleotide #746 of FIG. IA and #305 through nucleotide #493 of FIG. IB, (b) nucleotide #321 through nucleotide #1736 of FIG. II, (c) naturally occurring allelic sequences and equivalent degenerative codon sequences of (a) and (b); and (d) sequences which (1) hybridize to any of sequences (a) and (b) or (c) under stringent hybridization conditions; and (2)encode a protein characterized by the ability to induce the formation of bone and/or cartilage.
Claim 2
Dependent Claim
96 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising a coding sequence selected from the group consisting of (a) nucleostide #414 through nucleotide #746 of FIG. IA and #305 through nucleotide #493 of FIG. IB, (b) nucleotide #321 through nucleotide #1736 of FIG. II, (c) naturally occurring allelic sequences and equivalent degenerative codon sequences of (a) and (b); and (d) sequences which (1) hybridize to any of sequences (a) and (b) or (c) under stringent hybridization conditions; and (2)encode a protein characterized by the ability to induce the formation of bone and/or cartilage.
Claim 3
Dependent Claim
27 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nucleotide #414 through #746 of FIG. 1A and nucleotide #305 through #493 of FIG. 1B.
Claim 4
Dependent Claim
27 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nucleotide #414 through #746 of FIG. 1A and nucleotide #305 through #493 of FIG. 1B.
Claim 5
Dependent Claim
19 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nucleotide #321 through #1736 of FIG. 2.
Claim 6
Dependent Claim
19 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nucleotide #321 through #1736 of FIG. 2.
Claim 7
Dependent Claim
19 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nudeotide #1 through #1794 of FIG. 2.
Claim 8
Dependent Claim
19 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nudeotide #1 through #1794 of FIG. 2.
Claim 9
Dependent Claim
21 words
A vector comprising a DNA sequence of claim 1 in operative association with an expression control sequence for said DNA sequence.
Claim 10
Dependent Claim
21 words
A vector comprising a DNA sequence of claim 3 in operative association with an expression control sequence for said DNA sequence.
Claim 11
Dependent Claim
21 words
A vector comprising a DNA sequence of claim 4 in operative association with an expression control sequence for said DNA sequence.
Claim 12
Dependent Claim
27 words
A host cell transformed with the vector of claim 5 wherein said host cell is selected from the group consisting of mammalian, bacterial, yeast and insect cells.
Claim 13
Dependent Claim
10 words
A host cell transformed with the vector of claim 6.
Claim 14
Dependent Claim
10 words
A host cell transformed with the vector of claim 7.
Claim 15
Dependent Claim
11 words
The host cell of claim 9 wherein said host is mammalian.
Claim 16
Dependent Claim
11 words
The host cell of claim 10 wherein said host is mammalian.
Claim 17
Dependent Claim
39 words
A method of producing an osteoinductive protein, said method comprising the steps of (a) culturing in a suitable culture medium said transformed host cell of claim 8; and (b) isolating and purifying said osteoinductive protein from said culture medium.
Claim 18
Dependent Claim
13 words
The method of claim 13 wherein said host cell is a mammalian cell.
Claim 19
Dependent Claim
40 words
A method for producing an osteoinductive protein comprising the steps of: (a) culturing in a suitable culture medium a host cell transformed with the vector of claim 6; and (b) isolating and purifying said osteoinductive protein from said culture medium.
Claim 20
Dependent Claim
40 words
A method for producing an osteoinductive protein comprising the step of: (a) culturing in a suitable culture medium a host cell transformed with the vector of claim 7; and (b) isolating and purifying said osteoinductive protein from said culture medium.
Claim 21
Dependent Claim
13 words
The method of claim 15 wherein said host cell is a mammalian cell.
Claim 22
Dependent Claim
13 words
The method of claim 16 wherein said host cell, is a mammalian cell.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-05-26 | 1995-11-25 | 1996-05-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-05-26 | 1999-11-25 | 2000-05-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-05-26 | 2003-11-25 | 2004-05-26 | $7700.00 | Window Closed / Lapsed |