Patent US5116738A successfully refreshed from Google Patents.
US5116738A
Expired / Lapsed
US
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DNA sequences encoding
Assignee: Genetics Institute LLC
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Filing: 1991-04-26
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Granted: 1992-05-26
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Expires: 2011-04-26
Abstract
Abstract Purified BMP-3 proteins and processes for producing them are disclosed. They may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.
Inventors
- 👤 Elizabeth A. Wang
- 👤 John M. Wozney
- 👤 Vicki Rosen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:56
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A; A61; A61L; A61L24/00; A61L24/04; A61L24/043; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61K; A61K38/00
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
96 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising a coding sequence selected from the group consisting of (a) nucleostide #414 through nucleotide #746 of FIG. IA and #305 through nucleotide #493 of FIG. IB, (b) nucleotide #321 through nucleotide #1736 of FIG. II, (c) naturally occurring allelic sequences and equivalent degenerative codon sequences of (a) and (b); and (d) sequences which (1) hybridize to any of sequences (a) and (b) or (c) under stringent hybridization conditions; and (2)encode a protein characterized by the ability to induce the formation of bone and/or cartilage.
Claim 2
Dependent Claim
96 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising a coding sequence selected from the group consisting of (a) nucleostide #414 through nucleotide #746 of FIG. IA and #305 through nucleotide #493 of FIG. IB, (b) nucleotide #321 through nucleotide #1736 of FIG. II, (c) naturally occurring allelic sequences and equivalent degenerative codon sequences of (a) and (b); and (d) sequences which (1) hybridize to any of sequences (a) and (b) or (c) under stringent hybridization conditions; and (2)encode a protein characterized by the ability to induce the formation of bone and/or cartilage.
Claim 3
Dependent Claim
27 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nucleotide #414 through #746 of FIG. 1A and nucleotide #305 through #493 of FIG. 1B.
Claim 4
Dependent Claim
27 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nucleotide #414 through #746 of FIG. 1A and nucleotide #305 through #493 of FIG. 1B.
Claim 5
Dependent Claim
19 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nucleotide #321 through #1736 of FIG. 2.
Claim 6
Dependent Claim
19 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nucleotide #321 through #1736 of FIG. 2.
Claim 7
Dependent Claim
19 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nudeotide #1 through #1794 of FIG. 2.
Claim 8
Dependent Claim
19 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising nudeotide #1 through #1794 of FIG. 2.
Claim 9
Dependent Claim
21 words
A vector comprising a DNA sequence of claim 1 in operative association with an expression control sequence for said DNA sequence.
Claim 10
Dependent Claim
21 words
A vector comprising a DNA sequence of claim 3 in operative association with an expression control sequence for said DNA sequence.
Claim 11
Dependent Claim
21 words
A vector comprising a DNA sequence of claim 4 in operative association with an expression control sequence for said DNA sequence.
Claim 12
Dependent Claim
27 words
A host cell transformed with the vector of claim 5 wherein said host cell is selected from the group consisting of mammalian, bacterial, yeast and insect cells.
Claim 13
Dependent Claim
10 words
A host cell transformed with the vector of claim 6.
Claim 14
Dependent Claim
10 words
A host cell transformed with the vector of claim 7.
Claim 15
Dependent Claim
11 words
The host cell of claim 9 wherein said host is mammalian.
Claim 16
Dependent Claim
11 words
The host cell of claim 10 wherein said host is mammalian.
Claim 17
Dependent Claim
39 words
A method of producing an osteoinductive protein, said method comprising the steps of (a) culturing in a suitable culture medium said transformed host cell of claim 8; and (b) isolating and purifying said osteoinductive protein from said culture medium.
Claim 18
Dependent Claim
13 words
The method of claim 13 wherein said host cell is a mammalian cell.
Claim 19
Dependent Claim
40 words
A method for producing an osteoinductive protein comprising the steps of: (a) culturing in a suitable culture medium a host cell transformed with the vector of claim 6; and (b) isolating and purifying said osteoinductive protein from said culture medium.
Claim 20
Dependent Claim
40 words
A method for producing an osteoinductive protein comprising the step of: (a) culturing in a suitable culture medium a host cell transformed with the vector of claim 7; and (b) isolating and purifying said osteoinductive protein from said culture medium.
Claim 21
Dependent Claim
13 words
The method of claim 15 wherein said host cell is a mammalian cell.
Claim 22
Dependent Claim
13 words
The method of claim 16 wherein said host cell, is a mammalian cell.
References Cited (Backward Citations)
US4294753A
Cited
US4789732A
Cited
US4761471A
Cited
US4619989A
Cited
US4434094A
Cited
US4795804A
Cited
US4804744A
Cited
US4608199A
Cited
US4843063A
Cited
US4627982A
Cited
US4774228A
Cited
US4774322A
Cited
US4810691A
Cited
US4563350A
Cited
US4681763A
Cited
US4798885A
Cited
US4737578A
Cited
EP0336760A2
Cited
WO1989009787A2
Cited
WO1989009788A2
Cited
US4968590A
Cited
US5011691A
Cited
WO1990003733A1
Cited
CA2017466A1
Cited
EP0409472A1
Cited
WO1991002744A1
Cited
EP0416578A2
Cited
WO1991005802A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-05-26 | 1995-11-25 | 1996-05-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-05-26 | 1999-11-25 | 2000-05-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-05-26 | 2003-11-25 | 2004-05-26 | $7700.00 | Window Closed / Lapsed |