Patent US2527523A successfully refreshed from Google Patents.
US5166058A
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DNA sequences encoding the osteoinductive proteins
Assignee: Genetics Institute LLC
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Filing: 1989-07-11
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Granted: 1992-11-24
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Expires: 2009-07-11
Abstract
Abstract Purified BMP-2 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.
Inventors
- 👤 Elizabeth A. Wang
- 👤 John M. Wozney
- 👤 Vicki A. Rosen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:24
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A; A61; A61L; A61L24/00; A61L24/04; A61L24/043; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61K; A61K38/00
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
25 words
An isolated and purified DNA sequence encoding an osteoinductive protein said DNA sequence comprising the nucleotide sequence form nucleotide #356 to #1543 of FIG. 2.
Claim 2
Dependent Claim
25 words
An isolated and purified DNA sequence encoding an osteoinductive protein said DNA sequence comprising the nucleotide sequence form nucleotide #356 to #1543 of FIG. 2.
Claim 3
Dependent Claim
25 words
An isolated and purified DNA sequence encoding an osteoinductive protein said DNA sequence comprising the nucleotide sequence from nucleotide #403 to #1626 of FIG. 3.
Claim 4
Dependent Claim
25 words
An isolated and purified DNA sequence encoding an osteoinductive protein said DNA sequence comprising the nucleotide sequence from nucleotide #403 to #1626 of FIG. 3.
Claim 5
Dependent Claim
9 words
A vector comprising the DNA sequence of claim 1.
Claim 6
Dependent Claim
9 words
A vector comprising a DNA sequence of claim 2.
Claim 7
Dependent Claim
10 words
A host cell transformed with the vector of claim 3.
Claim 8
Dependent Claim
10 words
A host cell transformed with he vector of claim 4.
Claim 9
Dependent Claim
39 words
A method for producing a purified BMP-2A protein said method comprising the steps of (a) culturing in a suitable culture medium said transformed host cells of claim 5; and (b) isolating and purifying said BMP-2A from said culture medium.
Claim 10
Dependent Claim
39 words
A method for producing a purified BMP-2B protein said method comprising the steps of (a) culturing in a suitable culture medium said transformed host cells of claim 6; and (b) isolating and purifying said BMP-2B from said culture medium.
Claim 11
Independent Claim
49 words
A method for producing a purified BMP-2A protein said method comprising the steps of (a) culturing in a suitable culture medium a mammalian host cell transformed with a DNA sequence comprising nucleotide #356 through #1543 of FIG. 2; and (b) isolating and purifying said BMP-2A from said culture medium.
Claim 12
Independent Claim
49 words
A method for producing a purified BMP-2A protein said method comprising the steps of (a) culturing in a suitable culture medium a mammalian host cell transformed with a DNA sequence comprising nucleotide #356 through #1543 of FIG. 2; and (b) isolating and purifying said BMP-2A from said culture medium.
Claim 13
Dependent Claim
14 words
The method of claim 9 wherein said mammalian host cell is a CHO cell.
Claim 14
Independent Claim
49 words
A method for producing a purified BMP-2B protein said method comprising the steps of (a) culturing in a suitable culture medium a mammalian host cell transformed with a DNA sequence comprising nucleotide #403 through #1626 of FIG. 3; and (b) isolating and purifying said BMP-2B from said culture medium.
Claim 15
Independent Claim
49 words
A method for producing a purified BMP-2B protein said method comprising the steps of (a) culturing in a suitable culture medium a mammalian host cell transformed with a DNA sequence comprising nucleotide #403 through #1626 of FIG. 3; and (b) isolating and purifying said BMP-2B from said culture medium.
Claim 16
Dependent Claim
14 words
The method of claim 11 wherein said mammalian host cell is a CHO cell.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-11-24 | 1996-05-25 | 1996-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-11-24 | 2000-05-25 | 2000-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-11-24 | 2004-05-25 | 2004-11-24 | $7700.00 | Window Closed / Lapsed |