Patent US6040192A successfully refreshed from Google Patents.
US6040192A
Expired / Lapsed
US
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Method and means for magnetic particle specific binding assay
Assignee: Thermo Fisher Scientific Oy
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Filing: 1997-08-26
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Granted: 2000-03-21
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Expires: 2017-08-26
Abstract
Abstract A system and method for handling magnetic particles, as in an immunoassay. A remover having a magnetic body and covering sleeve is inserted in a vessel containing magnetic particles and a liquid. The remover is moved up and down with the particles adhered to agitate the liquid and improve adherence of the particles.
Inventors
- 👤 Jukka Tuunanen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 06:02
CPC Classifications
B; B03; B03C; B03C1/00; B03C1/02; B03C1/28; B03C1/286; B01; B01L; B01L3/00; B01L3/02; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/54366; G01N35/00; G01N35/02; G01N35/026; B01L2200/00; B01L2200/06; B01L2200/0647; B01L2200/0657; B01L2300/00; B01L2300/04; B01L2300/041; B01L2300/044; B01L2300/08; B01L2300/0809; B01L2300/0829; B01L2300/0848; B01L2300/16; B01L3/50; B01L3/508; B01L3/5085; B01L3/50853; B03C2201/00; B03C2201/18; G01N35/10; G01N2035/1027; G01N2035/1048; G01N2035/1055; G01N35/0098; G01N35/1065; G01N35/1081; G01N35/109; Y; Y10; Y10S; Y10S436/00; Y10S436/806; Y10S436/807; Y10S436/808; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
136 words
A method for determining the presence of an analyte in a sample, the method comprising the steps of: a. inserting a sample to be tested into a reaction vessel; b. inserting solid magnetic particles coated with separating reagent into said reaction vessel to react with the sample; c. removing said particles from said reaction vessel by inserting a magnet-equipped remover into said reacting vessel, wherein said particles adhere to said remover; d. inserting the remover containing the particles into a measuring vessel wherein at least one of said reaction and measuring vessels contains a medium and agitating the medium by moving said remover vertically while said particles adhere to said remover to improve adherence of said particles; e. measuring the reaction between the separating reagent and the sample; and f. determining the presence of the analyte.
Claim 2
Independent Claim
136 words
A method for determining the presence of an analyte in a sample, the method comprising the steps of: a. inserting a sample to be tested into a reaction vessel; b. inserting solid magnetic particles coated with separating reagent into said reaction vessel to react with the sample; c. removing said particles from said reaction vessel by inserting a magnet-equipped remover into said reacting vessel, wherein said particles adhere to said remover; d. inserting the remover containing the particles into a measuring vessel wherein at least one of said reaction and measuring vessels contains a medium and agitating the medium by moving said remover vertically while said particles adhere to said remover to improve adherence of said particles; e. measuring the reaction between the separating reagent and the sample; and f. determining the presence of the analyte.
Claim 3
Dependent Claim
38 words
The method of claim 1 further comprising the steps of: inserting the remover containing the particles into at least a third medium-containing vessel, agitating the medium in said third vessel and removing the particles from said third vessel.
Claim 4
Dependent Claim
20 words
The method of claim 1 wherein said steps include equipping said remover with a cover and reciprocating said cover-equipped remover.
Claim 5
Dependent Claim
24 words
The method of claim 1 wherein said reaction vessel contains the medium and a substance is attached to the medium in said reaction vessel.
Claim 6
Dependent Claim
29 words
The method of claim 4 wherein the sample reacts with the substance forming a reacted substance which binds any substance from the sample which may disturb later determining steps.
Claim 7
Independent Claim
95 words
A method for removing magnetic separating particles from a fluid, comprising: providing a magnetic remover and a vessel containing magnetic particles and said fluid, said remover and said vessel shaped and sized such that vertical insertion of said remover into said vessel causes substantial fluid flow vertically between an inner wall of said vessel and said remover, removing said magnetic particles from said vessel by inserting said remover into said vessel, agitating said fluid by moving said remover up and down while said particles adhere to said remover, and raising said remover from said vessel.
Claim 8
Independent Claim
95 words
A method for removing magnetic separating particles from a fluid, comprising: providing a magnetic remover and a vessel containing magnetic particles and said fluid, said remover and said vessel shaped and sized such that vertical insertion of said remover into said vessel causes substantial fluid flow vertically between an inner wall of said vessel and said remover, removing said magnetic particles from said vessel by inserting said remover into said vessel, agitating said fluid by moving said remover up and down while said particles adhere to said remover, and raising said remover from said vessel.
Claim 9
Dependent Claim
21 words
The method of claim 6 comprising agitation by repeatedly moving said remover up and down about 20 times in 10 seconds.
Claim 10
Dependent Claim
18 words
The method of claim 1 or 6 wherein the remover includes a sheath and a magnet insertable therein.
Claim 11
Dependent Claim
16 words
The method of claim 8 wherein the sheath and magnet are simultaneously inserted into said vessel.
Claim 12
Independent Claim
145 words
An apparatus for use in determining the presence of an analyte in a sample, the apparatus comprising: a. a reaction vessel containing a sample to be analyzed and solid magnetic particles coated with a separating reagent, wherein the separating reagent reacts with the sample; b. a plurality of vessels for performing intermediate steps on the particles after the reaction between the separating reagent and the sample; c. a measuring vessel for conducting measurements of the reaction between the separating reagent and the sample; d. a measuring device for measuring the reaction between the separating reagent and the sample in the measuring vessel; e. a magnet-equipped remover for inserting and moving the particles from one vessel to another, said remover being vertically movable for agitating a medium contained in each of said vessels while said particles adhere to said remover to improve adherence of said particles.
Claim 13
Independent Claim
145 words
An apparatus for use in determining the presence of an analyte in a sample, the apparatus comprising: a. a reaction vessel containing a sample to be analyzed and solid magnetic particles coated with a separating reagent, wherein the separating reagent reacts with the sample; b. a plurality of vessels for performing intermediate steps on the particles after the reaction between the separating reagent and the sample; c. a measuring vessel for conducting measurements of the reaction between the separating reagent and the sample; d. a measuring device for measuring the reaction between the separating reagent and the sample in the measuring vessel; e. a magnet-equipped remover for inserting and moving the particles from one vessel to another, said remover being vertically movable for agitating a medium contained in each of said vessels while said particles adhere to said remover to improve adherence of said particles.
Claim 14
Dependent Claim
14 words
The apparatus of claim 10 wherein the remover is equipped with an outer surface.
Claim 15
Dependent Claim
25 words
The apparatus of claim 11 wherein said outer surface has a profiled area for making the particles adhere to the remover as a dense mass.
Claim 16
Dependent Claim
11 words
The apparatus of claim 11 wherein said outer surface slopes downward.
Claim 17
Dependent Claim
17 words
The apparatus of claim 13 wherein said outer surface has a sharp nodule at its lowest point.
Claim 18
Dependent Claim
19 words
The apparatus of claim 10 wherein each of said vessels are joined together for form a single vessel unit.
Claim 19
Dependent Claim
18 words
The apparatus of claim 10 wherein at least one of said vessels is closed with a penetrable film.
Claim 20
Dependent Claim
15 words
The apparatus of claim 16 wherein each said vessel is closed with a penetrable film.
Claim 21
Dependent Claim
15 words
The apparatus of claim 16 wherein at least one closed vessel contains an inert gas.
Claim 22
Independent Claim
208 words
An apparatus for use in determining the presence of an analyte in a sample, the apparatus comprising: a. a reaction vessel containing a sample to be analyzed and solid magnetic particles coated with a separating reagent, wherein the separating reagent reacts with the sample; b. a plurality of vessels for performing intermediate steps on the particles after the reaction between the separating reagent and the sample; c. a measuring vessel for conducting measurements of the reaction between the separating reagent and the sample; d. a measuring device for measuring the reaction between the separating reagent and the sample in the measuring vessel; e. a remover for inserting, removing and moving the particles from one vessel to another, said remover containing a magnet for making said particles adhere to said remover after the reaction, said remover being equipped with an outer surface, said outer surface having a profiled area for making said particles adhere to said remover as a dense mass, said outer surface sloping downwardly, and said outer surface being a sharp nodule at its lowest point, wherein at least one vessel contains a medium, and said remover being vertically movable for agitating the medium while said particles adhere to said remover to improve adherence of said particles.
Claim 23
Independent Claim
208 words
An apparatus for use in determining the presence of an analyte in a sample, the apparatus comprising: a. a reaction vessel containing a sample to be analyzed and solid magnetic particles coated with a separating reagent, wherein the separating reagent reacts with the sample; b. a plurality of vessels for performing intermediate steps on the particles after the reaction between the separating reagent and the sample; c. a measuring vessel for conducting measurements of the reaction between the separating reagent and the sample; d. a measuring device for measuring the reaction between the separating reagent and the sample in the measuring vessel; e. a remover for inserting, removing and moving the particles from one vessel to another, said remover containing a magnet for making said particles adhere to said remover after the reaction, said remover being equipped with an outer surface, said outer surface having a profiled area for making said particles adhere to said remover as a dense mass, said outer surface sloping downwardly, and said outer surface being a sharp nodule at its lowest point, wherein at least one vessel contains a medium, and said remover being vertically movable for agitating the medium while said particles adhere to said remover to improve adherence of said particles.
Claim 24
Dependent Claim
18 words
The apparatus of claim 10 or 19 wherein the remover includes a sheath and a magnet insertable therein.
Claim 25
Dependent Claim
16 words
The apparatus of claim 20 wherein the sheath and magnet are simultaneously inserted into said vessel.
References Cited (Backward Citations)
US2471764A
Cited
GB1414479A
Cited
US3904482A
Cited
US3985649A
Cited
US4018886A
Cited
US3970518A
Cited
US4272510A
Cited
US4200613A
Cited
DE2824742A1
Cited
US4115535A
Cited
EP0027008A1
Cited
US4438068A
Cited
EP0042755A2
Cited
US4495151A
Cited
JPS585656A
Cited
JPS585657A
Cited
JPS585658A
Cited
GB2147698A
Cited
GB2147898A
Cited
US4649116A
Cited
US4731337A
Cited
US4751053A
Cited
EP0186001A2
Cited
WO1986006493A1
Cited
WO1987005536A1
Cited
JPS635263A
Cited
JPS635265A
Cited
JPS635266A
Cited
US4891321A
Cited
EP0317286A2
Cited
US4895650A
Cited
EP0351857A2
Cited
EP0358948A2
Cited
US5167926A
Cited
US5200084A
Cited
EP0479448A2
Cited
US5466574A
Cited
EP0522322A1
Cited
US5318914A
Cited
US5474742A
Cited
WO1994018564A1
Cited
WO1994018565A1
Cited
WO1995000247A1
Cited
US5647994A
Cited
WO1996012960A1
Cited
WO1996012958A1
Cited
WO1996012959A1
Cited
WO1996012961A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-03-21 | 2003-09-20 | 2004-03-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-03-21 | 2007-09-20 | 2008-03-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-03-21 | 2011-09-20 | 2012-03-21 | $7700.00 | Window Closed / Lapsed |