Patent US2890798A successfully refreshed from Google Patents.
EP0317286A2
Expired / Lapsed
EP
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Magnetic separation device and methods for use in heterogeneous assays
Assignee: BP Corp North America Inc
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Filing: 1988-11-16
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Granted: —
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Expires: 2008-11-16
Abstract
Abstract A separator device having a plurality of receiving orifice (3) for receiving microtubes. Preferably, the base (1) will be comprised of a nonferous or nonmagnetic material and may be advantageously machined out of a metal such as aluminium or molded out of suitable plastic. Most preferably, the materials shall be selected so that they may withstand typical sterilizing procedures such as autoclaving. Surrounding the periphery of orifices (3) are machined orifices (6) for receiving magnets (5), having a north-south field orientation which is coplanar with the surface of the base (1) and a cross-section of receiving orifice (3).
Inventors
- 👤 May K. Li
- 👤 Jack Kessler
- 👤 David T. Bach
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 13:02
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; B; B03; B03C; B03C1/00; B03C1/02; B03C1/28; B03C1/288; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5302; G01N33/543; G01N33/54313; G01N33/54326; B03C2201/00; B03C2201/26; G01N35/00; G01N35/10; G01N35/1009; G01N2035/1025; G01N35/0098; G01N35/1065
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
104 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means having a plurality of orifices for receiving nonferrous containers adapted to contain the said magnetic particles; b) a plurality of magnet means mounted on the said base spaced about the periphery of each receiving orifice wherein each of the said magnet means possess a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through the said receiving orifices and wherein each of the said north-south magnet fields of the said magnet means are oriented in a common direction.
Claim 2
Dependent Claim
104 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means having a plurality of orifices for receiving nonferrous containers adapted to contain the said magnetic particles; b) a plurality of magnet means mounted on the said base spaced about the periphery of each receiving orifice wherein each of the said magnet means possess a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through the said receiving orifices and wherein each of the said north-south magnet fields of the said magnet means are oriented in a common direction.
Claim 3
Dependent Claim
121 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means having a plurality of orifices for receiving nonferrous containers adapted to contain the said magnetic particles; b) a plurality of magnet means mounted on the said base spaced about the periphery of each receiving orifice wherein each of the said magnet means possess a north-south magnetic field orientation in a direction which is a coplanar with a cross-sectional plane through the said receiving orifices and wherein the north-south magnetic field direction orientation, moving in a common direction about the periphery of each of the said receiving orifices, alternates approximately 180° from the north-south field direction orientation of the preceding magnet.
Claim 4
Dependent Claim
121 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means having a plurality of orifices for receiving nonferrous containers adapted to contain the said magnetic particles; b) a plurality of magnet means mounted on the said base spaced about the periphery of each receiving orifice wherein each of the said magnet means possess a north-south magnetic field orientation in a direction which is a coplanar with a cross-sectional plane through the said receiving orifices and wherein the north-south magnetic field direction orientation, moving in a common direction about the periphery of each of the said receiving orifices, alternates approximately 180° from the north-south field direction orientation of the preceding magnet.
Claim 5
Dependent Claim
116 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means adapted to receive microwell tray means of nonferrous composition adapted to contain the said magnetic particles; b) a plurality of magnet means are mounted on the said base in spacial alignment with the said microwell tray means whereby each well in the said microwell tray means has adjacent thereto at least one magnet means possessing a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through the said well and where in each of the said north-south magnetic fields of the said magnet means are oriented in a common direction.
Claim 6
Dependent Claim
116 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means adapted to receive microwell tray means of nonferrous composition adapted to contain the said magnetic particles; b) a plurality of magnet means are mounted on the said base in spacial alignment with the said microwell tray means whereby each well in the said microwell tray means has adjacent thereto at least one magnet means possessing a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through the said well and where in each of the said north-south magnetic fields of the said magnet means are oriented in a common direction.
Claim 7
Dependent Claim
119 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means having a plurality of orifices for receiving nonferrous containers adapted to contain the said magnetic particles; b) a plurality of magnets mounted on the said base spaced about the periphery of each receiving orifice wherein each of the said magnets possess a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through the said receiving orifices and wherein the north-south magnet field direction of orientation, moving in a common direction about the periphery of each of the said receiving orifices, alternates approximately 180°C from the north-south field direction orientation of the preceding magnet.
Claim 8
Dependent Claim
119 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means having a plurality of orifices for receiving nonferrous containers adapted to contain the said magnetic particles; b) a plurality of magnets mounted on the said base spaced about the periphery of each receiving orifice wherein each of the said magnets possess a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through the said receiving orifices and wherein the north-south magnet field direction of orientation, moving in a common direction about the periphery of each of the said receiving orifices, alternates approximately 180°C from the north-south field direction orientation of the preceding magnet.
Claim 9
Dependent Claim
148 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means for receiving a nonferrous tray containing a plurality of microwells adapted to contain the said magnetic particles; b) cover means affording fluid removal holes and a plurality of magnet means mounted on the said cover and spaced on the said cover whereby engagement of the said cover with the said base and the said tray results in the juxtaposed placement of a plurality of magnet means about the periphery of each well, the fluid removal holes aligning with each well, wherein each of the said magnet means possess a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through the said well and wherein each of the said north-south magnetic fields of the said magnet means are oriented in a common direction.
Claim 10
Dependent Claim
148 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means for receiving a nonferrous tray containing a plurality of microwells adapted to contain the said magnetic particles; b) cover means affording fluid removal holes and a plurality of magnet means mounted on the said cover and spaced on the said cover whereby engagement of the said cover with the said base and the said tray results in the juxtaposed placement of a plurality of magnet means about the periphery of each well, the fluid removal holes aligning with each well, wherein each of the said magnet means possess a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through the said well and wherein each of the said north-south magnetic fields of the said magnet means are oriented in a common direction.
Claim 11
Dependent Claim
119 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means for receiving a nonferrous tray containing a plurality of microwells adapted to contain the said magnetic particles. b) base means comprising a plurality of magnet means mounted on the said base and spaced on the said base whereby engagement of the said base and the said tray results in the juxtaposed placement of a plurality of magnet means about the periphery of each well wherein the north-south magnetic field direction orientation, moving in a common direction about the periphery of each of the said wells, alternates approximately 180°C from the north-south field direction orientation of the preceding magnet.
Claim 12
Dependent Claim
119 words
A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, the said apparatus comprising: a) base means for receiving a nonferrous tray containing a plurality of microwells adapted to contain the said magnetic particles. b) base means comprising a plurality of magnet means mounted on the said base and spaced on the said base whereby engagement of the said base and the said tray results in the juxtaposed placement of a plurality of magnet means about the periphery of each well wherein the north-south magnetic field direction orientation, moving in a common direction about the periphery of each of the said wells, alternates approximately 180°C from the north-south field direction orientation of the preceding magnet.
Claim 13
Dependent Claim
30 words
Apparatus as claimed in any one of claims 1 to 6 in which each of the said receiving orifices or wells is surrounded about its periphery by four magnet means.
Claim 14
Dependent Claim
23 words
Apparatus as claimed in any one of claims 1 to 7 in which the said magnet means is a rare earth cobalt magnet.
Claim 15
Dependent Claim
19 words
Apparatus as claimed in claim 8 in which the said magnet means is a 33H Neodymium Iron Boron magnet.
Claim 16
Dependent Claim
48 words
Apparatus as claimed in any one of claims 1 to 9 further comprising tube transfer means adapted to receive nonferrous microtube containers and to mateably engage said base means whereby each nonferrous microtube container is received by a receiving orifice and is surrounded by a plurality of magnets.
Claim 17
Dependent Claim
73 words
A process for detecting a ligand in a fluid sample by immunoassay or hybridisation with a ligand specific binding substance which utilises a ferrous solid phase microparticle wherein the improvement comprises employing the magnetic separation apparatus as claimed in any one of claims 1 to 10 for effecting separation of solid phase bound ligands or ligand specific binding substances from unbound ligands or ligand specific binding substances present in the said fluid sample.
References Cited (Backward Citations)
No citation records indexed.
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