US4731337A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluorimetric immunological assay with magnetic particles
Assignee: Thermo Fisher Scientific Oy
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Filing: 1985-07-24
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Granted: 1988-03-15
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Expires: 2005-07-24
Abstract
Abstract Fluorometric immunological assay method in which an antigen is attached onto magnetic particles, which are, for the time of measurement, pulled against the wall of the measurement vessel by means of the magnetic field. The magnetic particles adhering to the vessel walls are irradiated and the resultant emitted fluorescence is measured.
Inventors
- 👤 Juhani E. I. Luotola
- 👤 Hannu Harjunmaa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 10:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S436/00; Y10S436/806; Y10S436/824
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
126 words
A method of immunological assay for a target antibody or target antigen comprising: a. attaching an antigen or antibody to solid magnetic particles; b. contacting the magnetic particles resulting from (a) with (1) a sample containing either the target antibody or target antigen, and (2) fluorescently labeled antibody or fluorescently labeled antigen, respectively, said contacting taking place in a measurement vessel; c. forcing the magnetic particles resulting from (b) against the wall of the measuring vessel by an applied magnetic field; d. irradiating, through the walls of the measuring vessel, the magnetic particles adhering to the wall as a result of (c); and e. measuring the resultant fluorescent or phosphorescent radiation with a detector after this radiation has passed through the walls of the measuring vessel.
Claim 2
Independent Claim
126 words
A method of immunological assay for a target antibody or target antigen comprising: a. attaching an antigen or antibody to solid magnetic particles; b. contacting the magnetic particles resulting from (a) with (1) a sample containing either the target antibody or target antigen, and (2) fluorescently labeled antibody or fluorescently labeled antigen, respectively, said contacting taking place in a measurement vessel; c. forcing the magnetic particles resulting from (b) against the wall of the measuring vessel by an applied magnetic field; d. irradiating, through the walls of the measuring vessel, the magnetic particles adhering to the wall as a result of (c); and e. measuring the resultant fluorescent or phosphorescent radiation with a detector after this radiation has passed through the walls of the measuring vessel.
Claim 3
Dependent Claim
32 words
Method as claimed in claim 1, characterized in that the solid magnetic particles to which the antigen or antibody is attached comprises polymer particles to which a magnetic substance has been added.
Claim 4
Dependent Claim
34 words
Method as claimed in claim 1, characterized in that, before measurement, a colouring agent is added in the measurement vessel, said colouring agent having a strong absorption at the wavelength of the exitation radiation.
Claim 5
Dependent Claim
34 words
Method as claimed in claim 1, characterized in that, before measurement, a colouring agent is added into the measurement vessel, said colouring agent having a strong absorption at the wavelength of the emission radiation.
Claim 6
Dependent Claim
14 words
Method as claimed in claim 3, characterized in that the colouring agent is black.
Claim 7
Dependent Claim
36 words
Method as claimed in claim 1, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 8
Dependent Claim
34 words
Method as claimed in claim 2, characterized in that, before measurement, a colouring agent is added into the measurement vessel, said colouring agent having a strong absorption at the wavelength of the excitation radiation.
Claim 9
Dependent Claim
34 words
Method as claimed in claim 2, characterized in that, before measurement, a colouring agent is added into the measurement vessel, said colouring agent having a strong absorption at the wavelength of the emission radiation.
Claim 10
Dependent Claim
34 words
Method as claimed in claim 3, characterized in that, before measurement, a colouring agent is added into the measurement vessel, said colouring agent having a strong absorption at the wavelength of the emission radiation.
Claim 11
Dependent Claim
34 words
Method as claimed in claim 7, characterized in that, before measurement, a colouring agent is added into the measurement vessel, said colouring agent having a strong absorption at the wavelength of the emission radiation.
Claim 12
Dependent Claim
14 words
Method as claimed in claim 4, characterized in that the colouring agent is black.
Claim 13
Dependent Claim
14 words
Method as claimed in claim 7, characterized in that the colouring agent is black.
Claim 14
Dependent Claim
14 words
Method as claimed in claim 8, characterized in that the colouring agent is black.
Claim 15
Dependent Claim
14 words
Method as claimed in claim 9, characterized in that the colouring agent is black.
Claim 16
Dependent Claim
14 words
Method as claimed in claim 10, characterized in that the colouring agent is black.
Claim 17
Dependent Claim
36 words
Method as claimed in claim 2, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 18
Dependent Claim
36 words
Method as claimed in claim 3, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 19
Dependent Claim
36 words
Method as claimed in claim 4, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 20
Dependent Claim
36 words
Method as claimed in claim 5, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 21
Dependent Claim
36 words
Method as claimed in claim 7, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 22
Dependent Claim
36 words
Method as claimed in claim 8, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 23
Dependent Claim
36 words
Method as claimed in claim 9, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 24
Dependent Claim
36 words
Method as claimed in claim 10, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 25
Dependent Claim
36 words
Method as claimed in claim 11, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 26
Dependent Claim
36 words
Method as claimed in claim 12, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 27
Dependent Claim
36 words
Method as claimed in claim 13, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 28
Dependent Claim
36 words
Method as claimed in claim 14, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
Claim 29
Dependent Claim
36 words
Method as claimed in claim 15, characterized in that both the excitation radiation is passed into the sample and the fluorescence or phosphorescence radiation is collected to the detector through the bottom of the measurement vessel.
References Cited (Backward Citations)
US4201763A
Cited
US4241176A
Cited
US4177253A
Cited
US4115535A
Cited
US4576912A
Cited
US4410660A
Cited
US4421860A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-03-15 | 1991-09-14 | 1992-03-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-03-15 | 1995-09-14 | 1996-03-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-03-15 | 1999-09-14 | 2000-03-15 | $7700.00 | Window Closed / Lapsed |