EP0351857A2
Expired / Lapsed
EP
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Immunoassay method using magnetic marker particles
Assignee: Olympus Corp
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Filing: 1989-07-20
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Granted: —
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Expires: 2009-07-20
Abstract
Abstract An immunoassay method using magnetic marker particles comprises the steps of mixing a sample and the marker particles which can be bonded specifically to or compete with to a substance to be measured to prepare a reaction solution thereof, collecting the marker particles in the reaction solution in a predetermined surface region in a measuring container (2), and measuring the substance to be measured in a sample on the basis of a distribution state of the marker particles collected in the predetermined wall surface area, characterized in that the marker particles are magnetic marker particles obtained by immobilizing on magnetic particles a marker substance which can be bonded specifically to or compete with the substance to be measured, and the magnet (4) is located a position corresponding to the predetermined wall surface region of the measuring container (2) outside the measuring container (2), thereby collecting the magnetic marker particles in the predetermined wall surface region.
Inventors
- 👤 Makoto Nakamura
- 👤 Toyohiro Tamai
- 👤 Ryouhei Shimizu
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 05:23
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5302; G01N33/5304; G01N33/543; G01N33/54313; G01N33/54326; G01N35/00; G01N35/0098
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
164 words
An immunoassay method using magnetic marker particles, comprising the steps of: mixing a sample and the marker particles which can be bonded specifically to or compete with a substance to be measured to prepare a reaction solution thereof; collecting the marker particles in the reaction solution within a predetermined surface region in a measuring container (2); and measuring the substance to be measured contained in the sample on the basis of a distribution state of the marker particles collected in the predetermined wall surface region, characterized in that the marker particles are magnetic marker particles obtained by immobilizing on magnetic particles a marker substance which can be bonded specifically to or compete with the substance to be measured, and applying a magnetic field from a magnet (4) to the reaction solution stored in a measuring container (2), the magnet being located outside a predetermined wall surface region of the measuring container (2), thereby collecting the magnetic marker particles in the predetermined wall surface region.
Claim 2
Dependent Claim
164 words
An immunoassay method using magnetic marker particles, comprising the steps of: mixing a sample and the marker particles which can be bonded specifically to or compete with a substance to be measured to prepare a reaction solution thereof; collecting the marker particles in the reaction solution within a predetermined surface region in a measuring container (2); and measuring the substance to be measured contained in the sample on the basis of a distribution state of the marker particles collected in the predetermined wall surface region, characterized in that the marker particles are magnetic marker particles obtained by immobilizing on magnetic particles a marker substance which can be bonded specifically to or compete with the substance to be measured, and applying a magnetic field from a magnet (4) to the reaction solution stored in a measuring container (2), the magnet being located outside a predetermined wall surface region of the measuring container (2), thereby collecting the magnetic marker particles in the predetermined wall surface region.
Claim 3
Dependent Claim
43 words
A method according to claim 1, characterized by further comprising the step of immobilizing the given substance which can specifically bond to or compete with the substance to be measured on at least the predetermined wall surface region of the measuring container (2).
Claim 4
Dependent Claim
56 words
A method according to claim 1, characterized by further comprising the steps of: immobilizing the given substance which can specifically bond to or compete with the substance to be measured on at least the predetermined wall surface region of the measuring container (2); and diluting the reaction solution of the sample and the magnetic marker particles.
Claim 5
Independent Claim
28 words
A method according to any one of claims 1 to 3, characterized in that the predetermined wall surface region is a bottom surface of the measuring container (2).
Claim 6
Independent Claim
24 words
A method according to any one of claims 1 to 4, characterized in that the predetermined wall surface region is a spherical wall surface.
Claim 7
Independent Claim
24 words
A method according to any one of claims 1 to 5, characterized in that the predetermined wall surface region is a conical wall surface.
Claim 8
Independent Claim
21 words
A method according to any one of claims 1 to 6, characterized in that the magnet is a needle-like magnet (8).
Claim 9
Dependent Claim
227 words
An immunoassay method using magnetic marker particles, comprising the steps of: immobilizing a given substance which can specifically bond to or compete with a substance to be measured on at least a predetermined wall surface region of a measuring container (2); adding a sample in the measuring contianer (2) to cause a reaction between the sample and the given substance, removing a sample, and washing the measuring container (2); adding to the washed measuring container a solution of the marker particles on which the marker substance which can be bonded specifically or compete with the substance to be measured is immobilized; collecting the marker particles in the solution within the predetermined wall surface region in the measuring container (2); and measuring the substance to be measured contained in the sample on the basis of a distribution state of the marker particles collected in the predetermined wall surface region, characterized in that the marker particles are magnetic marker particles obtained by immobilizing on magnetic paricles a marker substance which can be bonded specifically to or compete with the substance to be measured, and applying a magnetic field from a magnet (4) to the reaction solution stored in a measuring container (2), the magnet (2) being located outside a predetermined wall surface region of the measuring container, thereby collecting the magnetic marker particles in the predetermined wall surface region.
Claim 10
Dependent Claim
227 words
An immunoassay method using magnetic marker particles, comprising the steps of: immobilizing a given substance which can specifically bond to or compete with a substance to be measured on at least a predetermined wall surface region of a measuring container (2); adding a sample in the measuring contianer (2) to cause a reaction between the sample and the given substance, removing a sample, and washing the measuring container (2); adding to the washed measuring container a solution of the marker particles on which the marker substance which can be bonded specifically or compete with the substance to be measured is immobilized; collecting the marker particles in the solution within the predetermined wall surface region in the measuring container (2); and measuring the substance to be measured contained in the sample on the basis of a distribution state of the marker particles collected in the predetermined wall surface region, characterized in that the marker particles are magnetic marker particles obtained by immobilizing on magnetic paricles a marker substance which can be bonded specifically to or compete with the substance to be measured, and applying a magnetic field from a magnet (4) to the reaction solution stored in a measuring container (2), the magnet (2) being located outside a predetermined wall surface region of the measuring container, thereby collecting the magnetic marker particles in the predetermined wall surface region.
Claim 11
Dependent Claim
23 words
A method according to claim 8, characterized in that the predetermined wall surface region is a bottom surface of the measuring container (2).
Claim 12
Independent Claim
21 words
A method according to claim a or 9, characterized in that the predetermined wall surface region is a semispherical wall surface.
Claim 13
Independent Claim
24 words
A method according to any one of claims 8 to 10, characterized in that the predetermined wall surface region is a conical wall surface.
Claim 14
Dependent Claim
67 words
A reaction accelerating apparatus for immunoassay comprising: a supporting base (10) having an upper surface on which a plate having a plurality of reaction containers arranged thereon can be mounted; and a plurality of magnets (12, 14) arranged on said supporting base, said magnets being located on positions corresponding to those at which the containers are located when the plate is mounted on said supporting base (10).
Claim 15
Dependent Claim
67 words
A reaction accelerating apparatus for immunoassay comprising: a supporting base (10) having an upper surface on which a plate having a plurality of reaction containers arranged thereon can be mounted; and a plurality of magnets (12, 14) arranged on said supporting base, said magnets being located on positions corresponding to those at which the containers are located when the plate is mounted on said supporting base (10).
Claim 16
Dependent Claim
19 words
An apparatus according to claim 12, characterized in that said magnets (12) are in the shape of a needle.
Claim 17
Dependent Claim
21 words
An apparatus according to claim 12, characterized in that said magnets (14) are in the shape of a column or prism.
Claim 18
Independent Claim
51 words
An apparatus according to any one of claims 12 to 14, characterized in that male threads are cut in said magnets (14), female threads are cut in said supporting base, and these male and female threads are engaged, such that said magnets (14) can be fixed to the supporting base (10).
Claim 19
Independent Claim
31 words
An apparatus according to any one of claims 12 to 15, characterized in that the edge of said supporting base (10) is raised such that the plate can be reliably supported.
Claim 20
Dependent Claim
27 words
An apparatus according to claim 15, characterized in that either of grooves (15) 8 and ⊕ is carved on the head of each of said magnets (14).
References Cited (Backward Citations)
No citation records indexed.
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