US5501949A
Expired / Lapsed
US
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Particle bound binding component immunoassay
Assignee: Murex Diagnostics Corp
•
Filing: 1994-08-15
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Granted: 1996-03-26
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Expires: 2014-08-15
Abstract
Abstract An immunoassay method for the detection or quantitation of an analyte suspected of being in a solution comprising: (a) combining said specimen, a first binding component, insoluble particles, and second binding component labelled with a signal generating material in a solid phase retention and separation apparatus having a sufficient pore size such that said particles are trapped within said filter yet permitting rapid passage of fluid therethrough in such a manner that an immunological reaction occurs if analyte is present in said specimen, resulting in the formation of an immunocomplex of insolublized first binding component:analyte:second labelled binding component on or within said filter means; (b) separating bound from unbound material; and (c) determining the presence and/or amount of signal produced which is correlative with the amount of analyte present in the solution.
Inventors
- 👤 David L. Marshall
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 11:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/536; G01N33/537; G01N33/538; G01N33/54306; G01N33/569; G01N33/56911; G01N33/56927; Y; Y10; Y10S; Y10S435/00; Y10S435/97; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/815; Y10S436/817; Y10S436/818; Y10S436/824
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
202 words
A method for detection or quantitation of an analyte in a solution comprising the steps of: (a) contacting the solution with insoluble particles having attached thereto a binding component specific for the analyte, so as to form a suspension comprising a first complex wherein the first complex comprises the analyte, the binding component and the insoluble particle; (b) applying the suspension to at least a portion of a semi-permeable membrane having interstices of dimensions relative to the insoluble particles and having a thickness such that the insoluble particles are retained throughout the thickness of the semi-permeable membrane, the portion of the semi-permeable membrane retaining the insoluble particles defining an assay zone; (c) contacting the semi-permeable membrane containing the assay zone with a labeling component capable of specifically binding to the first complex so as to form a second complex wherein the second complex comprises the first complex and the labeling component, the semi-permeable membrane permitting the labeling component which is not bound to the first complex to pass out of the assay zone; and (d) measuring the signal produced by the labeling component of the second complex as an indicator of the presence or amount of the analyte present in the solution.
Claim 2
Independent Claim
202 words
A method for detection or quantitation of an analyte in a solution comprising the steps of: (a) contacting the solution with insoluble particles having attached thereto a binding component specific for the analyte, so as to form a suspension comprising a first complex wherein the first complex comprises the analyte, the binding component and the insoluble particle; (b) applying the suspension to at least a portion of a semi-permeable membrane having interstices of dimensions relative to the insoluble particles and having a thickness such that the insoluble particles are retained throughout the thickness of the semi-permeable membrane, the portion of the semi-permeable membrane retaining the insoluble particles defining an assay zone; (c) contacting the semi-permeable membrane containing the assay zone with a labeling component capable of specifically binding to the first complex so as to form a second complex wherein the second complex comprises the first complex and the labeling component, the semi-permeable membrane permitting the labeling component which is not bound to the first complex to pass out of the assay zone; and (d) measuring the signal produced by the labeling component of the second complex as an indicator of the presence or amount of the analyte present in the solution.
Claim 3
Dependent Claim
28 words
The method of claim 1, further comprising the step of applying to the semi-permeable membrane a wash fluid capable of passing through the semi-permeable membrane after Step (c).
Claim 4
Dependent Claim
12 words
The method of claim 1, wherein the semi-permeable membrane comprises a filter.
Claim 5
Dependent Claim
23 words
The method of claim 1, wherein the semi-permeable membrane comprises a material selected from the group consisting of glass, synthetic polymers and cellulose.
Claim 6
Dependent Claim
14 words
The method of claim 1, wherein the insoluble particles comprise an inert solid phase.
Claim 7
Dependent Claim
25 words
The method of claim 5, wherein the inert solid phase comprises a material selected form the group consisting of glass, cellulose, synthetic polymer, and protein.
Claim 8
Dependent Claim
26 words
The method of claim 5, wherein the binding component is covalently bound to the solid phase in an amount sufficient to substantially coat the solid phase.
Claim 9
Dependent Claim
21 words
The method of claim 5, wherein the binding component is bound to avidin and the solid phase is bound to biotin.
Claim 10
Dependent Claim
28 words
The method of claim 1, wherein the analyte is selected from the group consisting of antigens, antibodies, proteins, bacteria, fungi, haptens, hormones, parasites, viruses, and tumor cell markers.
Claim 11
Dependent Claim
11 words
The method of claim 1, wherein the analyte is an antigen.
Claim 12
Dependent Claim
28 words
The method of claim 10, wherein the antigen is selected from the group consisting of rubella virus, HTLV, cytomegalovirus, herpes virus, Chlamydia, Neisseria gonorrhea and human chorionic gonadotropin.
Claim 13
Dependent Claim
26 words
The method of claim 1, wherein the labeling component is selected form the group consisting of enzyme, bioluminescent material, chemiluminescent material, ferromagnetic material and radioactive material.
Claim 14
Dependent Claim
12 words
The method of claim 1, wherein the labeling component is alkaline phosphatase.
Claim 15
Independent Claim
178 words
A method for detection or quantitation of an analyte in a solution comprising the steps of: (a) contacting the solution with insoluble particles having attached thereto a binding component specific for the analyte so as to form a first complex wherein the first complex comprises the analyte, the binding component and the insoluble particle and with a labeling component capable of specifically binding to the first complex so as to form a suspension comprising a second complex, wherein the second complex comprises the first complex and the labeling component; (b) applying the suspension to at least a portion of a semi-permeable membrane having interstices of dimensions relative to the insoluble particles and having a thickness such that the insoluble particles are retained throughout the thickness of the semi-permeable membrane, the semi-permeable membrane permitting the soluble labeling component which is not bound to the insoluble particles to pass therethrough; and (c) measuring the signal produced by the labeling component of the second complex as an indicator of the presence or amount of the analyte present in the specimen solution.
Claim 16
Independent Claim
178 words
A method for detection or quantitation of an analyte in a solution comprising the steps of: (a) contacting the solution with insoluble particles having attached thereto a binding component specific for the analyte so as to form a first complex wherein the first complex comprises the analyte, the binding component and the insoluble particle and with a labeling component capable of specifically binding to the first complex so as to form a suspension comprising a second complex, wherein the second complex comprises the first complex and the labeling component; (b) applying the suspension to at least a portion of a semi-permeable membrane having interstices of dimensions relative to the insoluble particles and having a thickness such that the insoluble particles are retained throughout the thickness of the semi-permeable membrane, the semi-permeable membrane permitting the soluble labeling component which is not bound to the insoluble particles to pass therethrough; and (c) measuring the signal produced by the labeling component of the second complex as an indicator of the presence or amount of the analyte present in the specimen solution.
Claim 17
Dependent Claim
28 words
The method of claim 14, further comprising the step of applying to the semi-permeable membrane a wash fluid capable of passing through the semi-permeable membrane after Step (b).
Claim 18
Dependent Claim
12 words
The method of claim 14, wherein the semi-permeable membrane comprises a filter.
Claim 19
Dependent Claim
23 words
The method of claim 14, wherein the semi-permeable membrane comprises a material selected from the group consisting of glass, synthetic polymers and cellulose.
Claim 20
Dependent Claim
14 words
The method of claim 14, wherein the insoluble particles comprise an inert solid phase.
Claim 21
Dependent Claim
25 words
The method of claim 18, wherein the inert solid phase comprises a material selected form the group consisting of glass, cellulose, synthetic polymer, and protein.
Claim 22
Dependent Claim
26 words
The method of claim 18, wherein the binding component is covalently bound to the solid phase in an amount sufficient to substantially coat the solid phase.
Claim 23
Dependent Claim
21 words
The method of claim 18, wherein the binding component is bound to avidin and the solid phase is bound to biotin.
Claim 24
Dependent Claim
28 words
The method of claim 14, wherein the analyte is selected from the group consisting of antigens, antibodies, proteins, bacteria, fungi, haptens, hormones, parasites, viruses, and tumor cell markers.
Claim 25
Dependent Claim
11 words
The method of claim 14, wherein the analyte is an antigen.
Claim 26
Dependent Claim
28 words
The method of claim 23, wherein the antigen is selected from the group consisting of rubella virus, HTLV, cytomegalovirus, herpes virus, Chlamydia, Neisseria gonorrhea, and human chorionic gonadotropin.
Claim 27
Dependent Claim
26 words
The method of claim 14, wherein the labeling component is selected form the group consisting of enzymes, bioluminescent material, chemiluminescent material, ferromagnetic material and radioactive material.
Claim 28
Dependent Claim
12 words
The method of claim 14, wherein the labeling component is alkaline phosphatase.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-03-26 | 1999-09-25 | 2000-03-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-03-26 | 2003-09-25 | 2004-03-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-03-26 | 2007-09-25 | 2008-03-26 | $7700.00 | Window Closed / Lapsed |