US4415700A
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US
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Hydrophilic latex particles and use thereof
Assignee: TSCHECHOSLOWAKISCHE AKADEMIE DER WISENSCAFTEN
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Filing: 1981-12-16
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Granted: 1983-11-15
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Expires: 2001-12-16
Abstract
Abstract Hydrophilic latex particles consisting of a homo- or co-polymer of monomers which are sparingly soluble in water, which hydrophilic latex particles can be prepared by emulsion polymerization in the presence of a water-soluble, radical-forming initiator but without an addition of an emulsifier, stabilizer or wetting agent. A process for the preparation of these hydrophilic latex particles, wherein a monomer which is sparingly soluble in water or several monomers which are sparingly soluble in water are dispersed in water and, with the exclusion of oxygen, for example in an inert atmosphere, are homo- or co-polymerized by emulsion polymerization in the presence of a water-soluble, radical-forming initiator but without any addition of an emulsifier, stabilizer or wetting agent. A diagnostic agent containing these hydrophilic latex particles as carrier and biologically and/or immunologically active substances covalently bound to this carrier either directly or via a coupling agent as a bridge.
Inventors
- 👤 Hans-Georg Batz
- 👤 Paul Tanswell
- 👤 Manfred Baier
- 👤 Karel Bouchal
- 👤 Jaroslav Kalal
- 👤 Frantisek Svec
- 👤 Eva Zurkova
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:02
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; C; C08; C08F; C08F2/00; C08F2/12; C08F2/16; C08F2/22; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/74
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
34 words
Hydrophilic latex particles which are sparingly soluble in water consisting of a homo- or co-polymer of monomers containing at least one epoxy group and at least one polymerizable carbon-carbon double bond in the molecule.
Claim 2
Dependent Claim
34 words
Hydrophilic latex particles which are sparingly soluble in water consisting of a homo- or co-polymer of monomers containing at least one epoxy group and at least one polymerizable carbon-carbon double bond in the molecule.
Claim 3
Dependent Claim
32 words
Hydrophilic latex particles as claimed in claim 1, prepared by emulsion polymerization in the presence of a water soluble, radical-forming initiator and in the absence of an emulsifier, stabilizer or wetting agent.
Claim 4
Dependent Claim
23 words
Hydrophilic latex particles as claimed in claim 2, wherein the particles consist of a homo-polymer comprising at least 10% epoxide in the molecule.
Claim 5
Dependent Claim
41 words
Hydrophilic latex particles as claimed in claim 2, wherein the particles consist of a co-polymer wherein one of the monomers comprising at least 10% of the co-polymer, is an epoxide containing at least one polymerizable carbon-carbon double bond in the molecule.
Claim 6
Dependent Claim
19 words
Hydrophilic latex particles as claimed in claim 2, wherein at least one of the monomers is an epoxyalkylene compound.
Claim 7
Dependent Claim
22 words
Hydrophilic latex particles as claimed in claim 2, wherein at least one of the monomers is a glycidyl ester or glycidyl ether.
Claim 8
Dependent Claim
17 words
Hydrophilic latex particles as claimed in claim 6, wherein said ester is glycidyl acrylate or glycidyl methacrylate.
Claim 9
Dependent Claim
22 words
Hydrophilic latex particles as claimed in claim 2, wherein the homo- or co-polymer is cross-linked with a di- or polyunsaturated cross-linking agent.
Claim 10
Dependent Claim
29 words
Hydrophilic latex particles as claimed in claim 2, wherein the particles are monodisperse spheroids, all having substantially the same diameter in the range of from 0.15 to 1.5 μm.
Claim 11
Dependent Claim
25 words
Hydrophilic latex particles as claimed in claim 2, wherein the homo- or co-polymer has terminal hydroxyl, primary or secondary amino, thiol, aldehyde or carboxyl groups.
Claim 12
Dependent Claim
15 words
Hydrophilic latex particles as claimed in claim 2, also containing co-polymerized dyestuffs or fluorescing compounds.
Claim 13
Dependent Claim
18 words
Hydrophobic latex particles as claimed in claim 1 comprising 15% to 100% of said monomers containing epoxy groups.
Claim 14
Dependent Claim
60 words
Process for the preparation of hydrophilic latex particles as claimed in claim 1, which process comprises dispersing at least one monomer which is sparingly water soluble in water and, under the exclusion of oxygen, polymerizing same by emulsion polymerization in the presence of a water soluble radical forming initiator and in the absence of an emulsifier, stabilizer, or wetting agent.
Claim 15
Dependent Claim
16 words
Process as claimed in claim 13, wherein the polymerization is carried out in an inert atmosphere.
Claim 16
Dependent Claim
46 words
Process as claimed in claim 13, wherein an epoxide containing at least one (co)polymerizable carbon-carbon double bond in the molecule is dispersed in water alone or in admixture with other co-polymerizable monomers, and is homo- or co-polymerized by emulsion polymerization without the addition of an emulsifier.
Claim 17
Dependent Claim
22 words
Process as claimed in claim 15, wherein an epoxyalkylene compound or a glycidyl ester or glycidyl ether is used as the epoxide.
Claim 18
Dependent Claim
17 words
Process as claimed in claim 15, wherein glycidyl acrylate or glycidyl methacrylate is used as the epoxide.
Claim 19
Dependent Claim
23 words
Process as claimed in claim 13, wherein the emulsion polymerization is carried out with the addition of a di- or poly-unsaturated cross-linking agent.
Claim 20
Dependent Claim
27 words
Process as claimed in claim 13, wherein the emulsion polymerization is carried out with a bath ratio (water:monomer phase) of 8:1 to 16:1, referred to the volumes.
Claim 21
Dependent Claim
19 words
Process as claimed in claim 13, wherein the initiator concentration in the aqueous phase is 0.5 to 1.5 g./liter.
Claim 22
Dependent Claim
21 words
Process as claimed in claim 13, wherein the concentration of epoxide in the monomer phase is 1 to 100% by weight.
Claim 23
Dependent Claim
22 words
Process as claimed in claim 13, wherein the emulsion polymerization is carried out at a temperature of from 0° to 80° C.
Claim 24
Dependent Claim
60 words
Process as claimed in claim 13, wherein a latex containing terminal epoxide groups is, during or after the emulsion polymerization, subjected to hydrolysis, ammonolysis, aminolysis or thiolysis with aqueous alkali metal hydroxide solution, aqueous ammonia solution, with a primary amine, a hydrazine or a sulfide, terminal hydroxyl, amino groups, mono- or disubstituted amino groups or thiol groups thereby being formed.
Claim 25
Dependent Claim
24 words
The process of claim 23 further comprising subsequently reacting said groups, enzymatically, with periodate, or with periodic acid, to convert them into aldehyde groups.
Claim 26
Dependent Claim
27 words
Diagnostic agent containing hydrophilic latex particles as claimed in claim 1 as a carrier and also comprising biologically or immunologically active substance covalently bound to said particles.
Claim 27
Dependent Claim
19 words
Diagnostic agent as claimed in claim 25, wherein said covalent bonding to the carrier is via a coupling agent.
Claim 28
Dependent Claim
18 words
Diagnostic agent as claimed in claim 25, wherein said covalent bonding to the carrier is via direct coupling.
Claim 29
Dependent Claim
26 words
Diagnostic agent as claimed in claim 25, wherein the biologically and/or immunologically active substances are selected from peptides, proteins, enzymes, hormones, vitamins, antigens, antibodies and micro-organisms.
Claim 30
Dependent Claim
23 words
Diagnostic agents as claimed in claim 25 for the determination of thyroxine, containing a thyroxine antibody as said biologically or immunologically active substance.
Claim 31
Dependent Claim
25 words
Diagnostic agent as claimed in claim 25 for the determination of human thyreotropin, containing a human thyreotropin antibody as the biologically and/or immunologically active substance.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-14 | 1987-05-16 | 1987-11-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-14 | 1991-05-16 | 1991-11-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-14 | 1995-05-16 | 1995-11-15 | $7700.00 | Window Closed / Lapsed |