US4446238A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Bright field light microscopic method of localizing tissue antigens
Assignee: Janssen Pharmaceutica NV
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Filing: 1982-01-25
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Granted: 1984-05-01
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Expires: 2002-01-25
Abstract
Abstract The use of colloidal gold as a bright field light microscopic marker for the immunocytochemical localization of antigens in histological sections, namely the two step or the bridge immuno gold staining method.
Inventors
- 👤 Jan R. De Mey
- 👤 Marc K. J. J. Moeremans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 22:45
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/585; G01N1/00; G01N1/28; G01N1/30; G01N2474/00; G01N2474/20; Y; Y10; Y10S; Y10S436/00; Y10S436/801; Y10S436/805; Y10S436/807; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
70 words
A two step indirect bright field light microscopic method for the detection of antigens in tissue sections, which method comprises the steps of (i) preparing the tissue sections; (ii) reacting the tissue sections with primary antibodies specific to said antigens; (iii) detecting the specific binding sites, where the primary antibodies are attached, with colloidal gold granules coated with secondary antibodies specific to said primary antibodies; and (iv) mounting the sections.
Claim 2
Dependent Claim
70 words
A two step indirect bright field light microscopic method for the detection of antigens in tissue sections, which method comprises the steps of (i) preparing the tissue sections; (ii) reacting the tissue sections with primary antibodies specific to said antigens; (iii) detecting the specific binding sites, where the primary antibodies are attached, with colloidal gold granules coated with secondary antibodies specific to said primary antibodies; and (iv) mounting the sections.
Claim 3
Dependent Claim
98 words
A three step bridge bright field light microscopic method for the detection of antigens in tissue sections, which methods comprises the steps of (i) preparing the tissue sections; (ii) reacting the tissue sections with primary antibodies specific to said antigens; (iii) applying secondary unlabeled anti-antibodies in such an excess that they react with the primary antibodies with only one of their two combining sites; (iv) reacting the free sites of the said anti-antibodies with colloidal gold granules coated with γ-globulins, prepared from pre-immune serum of the same animal species as the primary antibodies; and (v) mounting the sections.
Claim 4
Dependent Claim
98 words
A three step bridge bright field light microscopic method for the detection of antigens in tissue sections, which methods comprises the steps of (i) preparing the tissue sections; (ii) reacting the tissue sections with primary antibodies specific to said antigens; (iii) applying secondary unlabeled anti-antibodies in such an excess that they react with the primary antibodies with only one of their two combining sites; (iv) reacting the free sites of the said anti-antibodies with colloidal gold granules coated with γ-globulins, prepared from pre-immune serum of the same animal species as the primary antibodies; and (v) mounting the sections.
References Cited (Backward Citations)
US3791932A
Cited
US4313734A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-01 | 1987-10-31 | 1988-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-01 | 1991-10-31 | 1992-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-01 | 1995-10-31 | 1996-05-01 | $7700.00 | Window Closed / Lapsed |