US4168146A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunoassay with test strip having antibodies bound thereto
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1976-01-15
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Granted: 1979-09-18
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Expires: 1996-01-15
Abstract
Abstract A test strip for carrying out immunoassays. The test strip is a bibulous carrier to which antibodies are bound. The immunoassay is carried out by wetting the test strip with the aqueous solution containing a suspected antigen. The test strip is then contacted with an aqueous solution containing labeled antibodies.
Inventors
- 👤 Anders O. Grubb
- 👤 Ulla C. Glad
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 00:46
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; Y; Y10; Y10S; Y10S435/00; Y10S435/97; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
86 words
In a method of immunochemical quantification, the improvement which comprises wetting a diagnostic test strip consisting essentially of a porous, capillarity-possessing carrier material having antibodies covalently bound to it, with an aqueous sample containing the antigen to be quantified, allowing the capillary migration to take place, and then assaying the antigen-containing area of said diagnostic test strip by wetting it with antibodies in an aqueous vehicle, said antibodies being bound to a water-soluble fluorescent color indicating compound or to an enzyme that catalyzes a color-developing reaction.
Claim 2
Dependent Claim
86 words
In a method of immunochemical quantification, the improvement which comprises wetting a diagnostic test strip consisting essentially of a porous, capillarity-possessing carrier material having antibodies covalently bound to it, with an aqueous sample containing the antigen to be quantified, allowing the capillary migration to take place, and then assaying the antigen-containing area of said diagnostic test strip by wetting it with antibodies in an aqueous vehicle, said antibodies being bound to a water-soluble fluorescent color indicating compound or to an enzyme that catalyzes a color-developing reaction.
Claim 3
Dependent Claim
24 words
The method as claimed in claim 1, wherein the antibodies in the aqueous vehicle are bound to an enzyme that catalyzes a color-developing reaction.
Claim 4
Dependent Claim
13 words
The method as claimed in claim 2, wherein the enzyme is lactic dehydrogenase.
Claim 5
Dependent Claim
23 words
The method as claimed in claim 1, wherein the antibodies in the aqueous vehicle are bound to a water-soluble fluorescent color indicating compound.
Claim 6
Dependent Claim
29 words
The method improvement as claimed in claim 4, wherein the diagnostic test strip is composed of a polyvinyl chloride-silica combination onto which have been adsorbed antibodies against human gammaglobulin.
Claim 7
Dependent Claim
19 words
The method improvement as claimed in claim 4, wherein the color indicator compound is fluoresceinisothiocyanate, rhodamine, or dansyl chloride.
Claim 8
Dependent Claim
15 words
The method improvement as claimed in claim 6, wherein the antibody-bound color indicator is fluoresceinisothiocyanate.
Claim 9
Dependent Claim
56 words
A diagnostic test device useful for immunochemical quantification, which is substantially a carrier strip comprising a silica-modified micro-porous polymer having finely divided silica substantially uniformly embedded in a permeable, continuous polymeric matrix selected from the group consisting of polyvinyl chloride, vinyl chloride-propylene copolymer, and vinyl chloride vinyl acetate copolymer, said strip having antibodies bound to it.
Claim 10
Dependent Claim
56 words
A diagnostic test device useful for immunochemical quantification, which is substantially a carrier strip comprising a silica-modified micro-porous polymer having finely divided silica substantially uniformly embedded in a permeable, continuous polymeric matrix selected from the group consisting of polyvinyl chloride, vinyl chloride-propylene copolymer, and vinyl chloride vinyl acetate copolymer, said strip having antibodies bound to it.
Claim 11
Dependent Claim
19 words
The diagnostic test device as claimed in claim 8, wherein the carrier material is a porous polyvinyl chloride matrix.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-09-17 | 1983-03-19 | 1983-09-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-09-17 | 1987-03-19 | 1987-09-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-09-17 | 1991-03-19 | 1991-09-18 | $7700.00 | Window Closed / Lapsed |