US3876504A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Procedure for determination of antigens and antibodies and articles for use therewith
Assignee: Early Warning Co
•
Filing: 1974-06-10
•
Granted: 1975-04-08
•
Expires: 1994-06-10
Abstract
Abstract The invention relates to a test slide to be employed in the determination of antigens or antibodies, particularly antigens or antibodies in human body fluids, and a procedure for use of the slide. The procedure involves bonding of one component of the antigen-antibody reaction group to an insoluble member on the test slide and flooding to this test slide with a mixture of the other component, bonded to a color-forming agent, and a body fluid which may contain the other component.
Inventors
- 👤 David Koffler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 23:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; G01N33/523; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/964; Y10S436/00; Y10S436/815; Y10S436/817; Y10S436/818
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
100 words
AN ARTICLE FOR USE IN DETERMINING THE PRESENCE OF ONE COMPONENT OF THE ANTIGEN-ANTIBODY REACTION MEMBER IN A HUMAN BODY FLUID, SAID ARTICLE COMPRISING: A. A TEST SLIDE FORMED OF A MATERIAL INERT TO THE REACTANTS UNDER THE CONDITIONS OF REACTION; B. A FIRST COATING ON SAID TEST SLIDE FORMED FROM GELATIN AND A MATERIAL WHICH, UPON DRYING, FORMS A POROUS SURFACE AND C. A SECOND COATING OF GELATIN AND A MATERIAL WHICH, UPON DRYING, FORMS A POROUS SURFACE; AND, A SMALL QUANTITY OF A CONJUGATE OF CYANOGEN BROMIDE TREATED SEPHAROSE AND THE OTHER COMPONENT OF THE ANTIGEN-ANTIBODY REACTION MEMBER.
Claim 2
Dependent Claim
100 words
AN ARTICLE FOR USE IN DETERMINING THE PRESENCE OF ONE COMPONENT OF THE ANTIGEN-ANTIBODY REACTION MEMBER IN A HUMAN BODY FLUID, SAID ARTICLE COMPRISING: A. A TEST SLIDE FORMED OF A MATERIAL INERT TO THE REACTANTS UNDER THE CONDITIONS OF REACTION; B. A FIRST COATING ON SAID TEST SLIDE FORMED FROM GELATIN AND A MATERIAL WHICH, UPON DRYING, FORMS A POROUS SURFACE AND C. A SECOND COATING OF GELATIN AND A MATERIAL WHICH, UPON DRYING, FORMS A POROUS SURFACE; AND, A SMALL QUANTITY OF A CONJUGATE OF CYANOGEN BROMIDE TREATED SEPHAROSE AND THE OTHER COMPONENT OF THE ANTIGEN-ANTIBODY REACTION MEMBER.
Claim 3
Dependent Claim
16 words
The article of claim 1 wherein the material which forms the porous surface is chrome alum.
Claim 4
Dependent Claim
15 words
The article of claim 1 wherein the material which forms the porous surface is agarose.
Claim 5
Dependent Claim
15 words
The article of claim 1 wherein the material which forms the porous surface is agar.
Claim 6
Dependent Claim
19 words
The article of claim 1 wherein the component conjugated to the cyanogen bromide treated Sepharose is bovine serum albumin.
Claim 7
Dependent Claim
19 words
The article of claim 1 wherein the component conjugated to the cyanogen bromide treated Sepharose is human gamma globulin.
Claim 8
Dependent Claim
19 words
The article of claim 1 wherein the component conjugated to the cyanogen bromide treated Sepharose is human chorionic gonadotrophin.
Claim 9
Dependent Claim
18 words
The article of claim 1 wherein the component bonded to the cyanogen bromide treated Sepharose is the antigen.
Claim 10
Dependent Claim
76 words
A procedure for determining the presence of one component of an antigen-antibody reaction member comprising: a. forming a test slide article according to claim 1; b. applying to said article a mixture of a human body fluid and a conjugate formed from the other component and an enzyme which will activate a coloring component; c. rinsing said article with distilled water; and d. applying a material which is activatable by said enzyme to produce a color.
Claim 11
Dependent Claim
16 words
The article of claim 9 wherein the material which forms the porous surface is chrome alum.
Claim 12
Dependent Claim
15 words
The article of claim 9 wherein the material which forms the porous surface is agarose.
Claim 13
Dependent Claim
15 words
The article of claim 9 wherein the material which forms the porous surface is agar.
Claim 14
Dependent Claim
19 words
The article of claim 9 wherein the component conjugated to the cyanogen bromide treated Sepharose is bovine serum albumin.
Claim 15
Dependent Claim
19 words
The article of claim 9 wherein the component conjugated to the cyanogen bromide treated Sepharose is human gamma globulin.
Claim 16
Dependent Claim
19 words
The article of claim 9 wherein the component conjugated to the cyanogen bromide treated Sepharose is human chorionic gonadotrophin.
Claim 17
Dependent Claim
18 words
The article of claim 9 wherein the component bonded to the cyanogen bromide treated Sepharose is the antigen.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-07 | 1978-10-07 | 1979-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-07 | 1982-10-07 | 1983-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-07 | 1986-10-07 | 1987-04-08 | $7700.00 | Window Closed / Lapsed |