US5371208A
Expired / Lapsed
US
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Preparation of cross-linked linear polysaccharide polymers as gels for electrophoresis
Assignee: ELCHROM SCIENTIFIC Ltd
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Filing: 1992-12-30
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Granted: 1994-12-06
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Expires: 2012-12-30
Abstract
Abstract A bed of transparent, water insoluble, charge-free crosslinked gel which is suitable for use in electrophoresis is prepared. The gel is formed by dissolving a linear polysaccharide, such as agarose, in a suitable solvent, such as water. This dissolution may be accomplished with agitation. A suitable cross linking agent, which is not itself charged, nor does it become charged upon contact with water, at a pH of 2 to 12, is then added to the solution, possibly with agitation in order to get thorough mixing of the linear polysaccharide and the cross linking agent. This mixture is then allowed to incubate and react in a quiescent state to substantially simultaneously gel and crosslink to form the desired cross linked gel bed. Preferred cross-linking agents are bis-epoxides, halo-epoxides, bis-haloalkanes, bis-halo-alcohols, alkanediol-bis-alkyl sulfonates, alkanediol-bis-aryl sulfonates or divinylsulfone. The liner polysaccharide maybe mixed with a synthetic polymer such as polyvinyl alcohol.
Inventors
- 👤 Branko Kozulic
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 06:15
CPC Classifications
C; C08; C08J; C08J3/00; C08J3/24; B; B01; B01D; B01D57/00; B01D57/02; C08B; C08B15/00; C08B15/005; C08B31/00; C08B31/003; C08B37/00; G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; C08J2301/00; C08J2305/00
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
187 words
A method of preparing a substantially continuous bed of a water insoluble, substantially transparent gel comprising: mixing together with an aqueous solvent: a composition comprising at least one polymer containing hydroxyl groups, said composition comprising a linear polysaccharide; and a crosslinking agent comprising at least one compound which is substantially devoid of functional groups which are charged, or which become charged upon contact with water, in the pH range of about 2 to 11, and which is able to react, through at least two reactive groups each of which is selected from the group consisting of epoxides, halides, sulfonates and activated olefins, with hydroxyl groups of said linear polysaccharide to form ether linkages therewith to form a mixture; subjecting said mixture comprising said linear polysaccharide and crosslinking agent in said aqueous solvent to crosslinking reaction conditions sufficient to: react reactive groups of said crosslinking agent with hydroxyl groups of said polysaccharide to form an ether linked crosslinked product which is substantially devoid of said charged groups, and gel said crosslinked polymer substantially simultaneously with said crosslinking reaction, into a bed of water insoluble, substantially transparent, crosslinked gel.
Claim 2
Independent Claim
187 words
A method of preparing a substantially continuous bed of a water insoluble, substantially transparent gel comprising: mixing together with an aqueous solvent: a composition comprising at least one polymer containing hydroxyl groups, said composition comprising a linear polysaccharide; and a crosslinking agent comprising at least one compound which is substantially devoid of functional groups which are charged, or which become charged upon contact with water, in the pH range of about 2 to 11, and which is able to react, through at least two reactive groups each of which is selected from the group consisting of epoxides, halides, sulfonates and activated olefins, with hydroxyl groups of said linear polysaccharide to form ether linkages therewith to form a mixture; subjecting said mixture comprising said linear polysaccharide and crosslinking agent in said aqueous solvent to crosslinking reaction conditions sufficient to: react reactive groups of said crosslinking agent with hydroxyl groups of said polysaccharide to form an ether linked crosslinked product which is substantially devoid of said charged groups, and gel said crosslinked polymer substantially simultaneously with said crosslinking reaction, into a bed of water insoluble, substantially transparent, crosslinked gel.
Claim 3
Dependent Claim
23 words
A method as claimed in claim 1 including carrying out said substantially simultaneous cross-linking and gelation without any substantial agitation of said solution.
Claim 4
Dependent Claim
31 words
The method as claimed in claim 1 wherein said cross linking agent comprises a member selected from the group consisting of bis-epoxides, halo-epoxides, bis-haloalkanes, bis-halo-alcohols, alkanediol-bis-alkyl sulfonates, alkanediol-bis-aryl sulfonates, and divinylsulfone.
Claim 5
Dependent Claim
9 words
Method of claim 1, wherein the polysaccharide comprises agarose.
Claim 6
Dependent Claim
11 words
A method of claim 1, wherein the polysaccharide comprises hydroxyethyl cellulose.
Claim 7
Dependent Claim
11 words
A method of claim 1, wherein the polysaccharide comprises hydroxyethyl agarose.
Claim 8
Dependent Claim
15 words
A method of claim 1, wherein said polysaccharide comprises a linear and a branched polysaccharide.
Claim 9
Dependent Claim
17 words
A method of claim 7, wherein the linear polysaccharide comprises agarose and the branched polysaccharide comprises dextran.
Claim 10
Dependent Claim
17 words
A method of claim 7, wherein the linear polysaccharide comprises agarose and the branched polysaccharide comprises starch.
Claim 11
Dependent Claim
14 words
A method of claim 1, wherein said polysaccharide comprises more than one linear polysaccharide.
Claim 12
Dependent Claim
13 words
A method of claim 10, wherein said polysaccharide comprises agarose and hydroxyethyl cellulose.
Claim 13
Dependent Claim
14 words
A method of claim 7, wherein said polysaccharide comprises more than one branched polysaccharide.
Claim 14
Dependent Claim
14 words
A method of claim 12, wherein said polysaccharide comprises agarose, hydroxyethyl cellulose and dextran.
Claim 15
Dependent Claim
14 words
A method of claim 12, wherein said polysaccharide comprises agarose, hydroxyethyl cellulose and starch.
Claim 16
Independent Claim
23 words
A method according to any one of claims from 4 to 14 wherein said composition additionally contains a synthetic polymer with hydroxyl groups.
Claim 17
Dependent Claim
12 words
A method of claim 15, wherein the synthetic polymer is polyvinyl alcohol.
Claim 18
Dependent Claim
30 words
A method according to claim 16, wherein the cross-linking agent is selected from a group of compounds consisting of dihaloalkyl alcohols, halohydrins, bisepoxides, divinyl sulfone, and alkanediol dialkyl or diarylsulfonates.
Claim 19
Dependent Claim
23 words
A method of claim 17, including carrying out said substantially simultaneous cross-linking reaction and gel formation at pH values of 8 to 14.
Claim 20
Dependent Claim
23 words
A method of claim 17, including carrying out said substantially simultaneous cross-linking reaction and gel formation at temperatures of 4° to 65° C.
Claim 21
Dependent Claim
23 words
A method of claim 17, including carrying out said substantially simultaneous cross-linking reaction and gel formation for about 15 minutes to 5 days.
Claim 22
Dependent Claim
24 words
A method according to claim 16, including carrying out said substantially simultaneous cross-linking reaction and gel formation in a solvent consisting essentially of water.
Claim 23
Dependent Claim
29 words
A method according to claim 16, including carrying out said substantially simultaneous cross-linking reaction and gel formation in a solvent comprising a mixture of water and an organic solvent.
Claim 24
Dependent Claim
14 words
A method according to claim 1, further including fixing said gel to a support.
Claim 25
Dependent Claim
15 words
The method as claimed in claim 1 wherein said aqueous solvent consists essentially of water.
Claim 26
Dependent Claim
51 words
The method as claimed in claim 1 wherein said polymer of said composition comprises a member selected from the group consisting of hydroxyethyl cellulose, agar, agarose, gellan, pullulan, starch, dextran and a mixture of a linear polysaccharide with one member selected from the group consisting of polyvinyl alcohol and polyhydroxyethyl methacrylate.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1997-12-05 | 1998-06-06 | 1998-12-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-12-05 | 2002-06-06 | 2002-12-06 | $3760.00 | Window Closed / Lapsed |
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