US4459358A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Multilayer element for analysis
Assignee: Polaroid Corp
•
Filing: 1982-12-29
•
Granted: 1984-07-10
•
Expires: 2002-12-29
Abstract
Abstract A multilayer element for the analysis of a liquid containing an analyte comprising a reaction layer which retains a predetermined amount of a diffusible reagent containing a detectable species; said reagent adapted to be rendered substantially non-diffusible by said analyte as a function of the concentration of said analyte; a registration layer adapted to receive reagent diffusing thereto; means for detecting said reagent in said registration layer and an insulating layer intermediate said reaction layer adapted to provide discrimination between detectable species in said reagent layer and said registration layer.
Inventors
- 👤 Carl M. Berke
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:56
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N31/00; G01N31/22; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
108 words
A multilayer element for the analysis of a liquid containing an analyte comprising a reaction layer which retains a predetermined amount of a diffusible reagent containing a detectable species, said diffusible reagent being present in an amount in excess of that reactable with an analyte to be determined; said reagent adapted to be rendered substantially non-diffusible by said analyte as a function of the concentration of said analyte; a registration layer adapted to receive reagents diffusing thereto; and an insulating layer permeable to said diffusible reagent intermediate said reaction layer and said registration layer, said insulating layer preventing detection of the detectable species retained in the reaction layer.
Claim 2
Dependent Claim
108 words
A multilayer element for the analysis of a liquid containing an analyte comprising a reaction layer which retains a predetermined amount of a diffusible reagent containing a detectable species, said diffusible reagent being present in an amount in excess of that reactable with an analyte to be determined; said reagent adapted to be rendered substantially non-diffusible by said analyte as a function of the concentration of said analyte; a registration layer adapted to receive reagents diffusing thereto; and an insulating layer permeable to said diffusible reagent intermediate said reaction layer and said registration layer, said insulating layer preventing detection of the detectable species retained in the reaction layer.
Claim 3
Dependent Claim
18 words
The element of claim 1 which includes a sample spreading layer for receiving the liquid to be analyzed.
Claim 4
Dependent Claim
15 words
The element of claim 2 wherein said spreading layer is an isotropically non-fibrous spreading layer.
Claim 5
Dependent Claim
11 words
The element of claim 2 wherein said spreading layer is fibrous.
Claim 6
Dependent Claim
13 words
The element of claim 1 wherein said detectable species is a fluorescent material.
Claim 7
Dependent Claim
13 words
The element of claim 1 wherein said detectable species is a radioactive material.
Claim 8
Dependent Claim
18 words
The element of claim 1 wherein said insulating layer is opaque to the radiation from said detectable species.
Claim 9
Dependent Claim
25 words
The element of claim 1 wherein said liquid contains antigen (antibody) and said reaction layer contains diffusible antibody (antigen) with a detectable species attached thereto.
Claim 10
Dependent Claim
12 words
The element of claim 2 wherein said spreading layer is a fabric.
Claim 11
Dependent Claim
11 words
The element of claim 2 wherein said reaction layer comprises agarose.
Claim 12
Dependent Claim
12 words
The element of claim 1 wherein said insulating layer comprises titanium dioxide.
Claim 13
Dependent Claim
11 words
The element of claim 1 wherein said registration layer comprises agarose.
Claim 14
Dependent Claim
12 words
The element of claim 1 wherein said registration layer includes polymeric emulsion.
Claim 15
Dependent Claim
13 words
The element of claim 1 including a radiation transparent base carrying said layers.
Claim 16
Dependent Claim
10 words
The element of claim 14 wherein said base is polystyrene.
Claim 17
Independent Claim
101 words
A method for analyzing an analyte in a liquid which comprises adding said liquid to an element comprising a plurality of layers including a reaction layer and a registration layer; said reaction layer containing a predetermined amount of a diffusible reagent containing a detectable species, said diffusible reagent being present in an amount in excess of that reactable with an analyte to be determined; said reagent being rendered non-diffusible by said analyte as a function of the concentration of said analyte; said registration layer adapted to receive reagent diffusing thereto; and measuring the quantity of reagent diffusion to said registration layer.
Claim 18
Independent Claim
101 words
A method for analyzing an analyte in a liquid which comprises adding said liquid to an element comprising a plurality of layers including a reaction layer and a registration layer; said reaction layer containing a predetermined amount of a diffusible reagent containing a detectable species, said diffusible reagent being present in an amount in excess of that reactable with an analyte to be determined; said reagent being rendered non-diffusible by said analyte as a function of the concentration of said analyte; said registration layer adapted to receive reagent diffusing thereto; and measuring the quantity of reagent diffusion to said registration layer.
References Cited (Backward Citations)
US3723064A
Cited
US3992158A
Cited
US4066403A
Cited
US4042335A
Cited
US4050898A
Cited
US4069016A
Cited
US4144306A
Cited
US4166093A
Cited
US4258001A
Cited
US4292272A
Cited
EP0051183A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-07-10 | 1988-01-09 | 1988-07-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-07-10 | 1992-01-09 | 1992-07-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-07-10 | 1996-01-09 | 1996-07-10 | $7700.00 | Window Closed / Lapsed |