US4166093A
Expired / Lapsed
US
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Reduction of detectable species migration in elements for the analysis of liquids
Assignee: Eastman Kodak Co
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Filing: 1978-06-16
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Granted: 1979-08-28
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Expires: 1998-06-16
Abstract
Abstract An element for the analysis of liquids contains a radiation-transmissive, detectable species migration-inhibiting layer interposed between a porous radiation-blocking layer and a radiation-transmissive reagent layer. All three layers are permeable to a predetermined analyte. The reagent layer contains a composition that provides a detectable species such as a dye in proportion to the concentration of the analyte that diffuses into the reagent layer from the overlying porous radiation-blocking layer. The detectable species migration-inhibiting layer acts to reduce the migration of, for example, dye from the reagent layer into the porous radiation-blocking layer, where the optical density of the dye cannot easily be measured. Optionally, the above-described three layers can be carried on a radiation-transmissive support, and other layers such as spreading layers, registration layers, and subbing layers can also be present in the element.
Inventors
- 👤 Margaret J. Smith-Lewis
- 👤 John Figueras
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:37
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; G01N33/84
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
102 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a radiometrically detectable species, and a porous radiation-blocking layer permeable to said analyte; the improvement comprising a radiation-transmissive, detectable species migration-inhibiting layer interposed between the reagent layer and the porous radiation-blocking layer, said detectable species migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said radiometrically detectable species to said porous radiation-blocking layer upon contact of said element with the liquid under analysis.
Claim 2
Dependent Claim
102 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a radiometrically detectable species, and a porous radiation-blocking layer permeable to said analyte; the improvement comprising a radiation-transmissive, detectable species migration-inhibiting layer interposed between the reagent layer and the porous radiation-blocking layer, said detectable species migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said radiometrically detectable species to said porous radiation-blocking layer upon contact of said element with the liquid under analysis.
Claim 3
Dependent Claim
22 words
An element as described in claim 1 wherein said detectable species migration-inhibiting layer comprises an immobilized antibody for said radiometrically detectable species.
Claim 4
Dependent Claim
96 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a dye, and a porous radiation-blocking layer permeable to said analyte; the improvement comprising a radiation-transmissive, dye migration-inhibiting layer interposed between the reagent layer and the porous radiation-blocking layer, said dye migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said dye to said porous radiation-blocking layer upon contact of said element with the liquid under analysis.
Claim 5
Dependent Claim
96 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a dye, and a porous radiation-blocking layer permeable to said analyte; the improvement comprising a radiation-transmissive, dye migration-inhibiting layer interposed between the reagent layer and the porous radiation-blocking layer, said dye migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said dye to said porous radiation-blocking layer upon contact of said element with the liquid under analysis.
Claim 6
Dependent Claim
119 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a radiometrically detectable species, a spreading layer permeable to said analyte, and a porous radiation-blocking layer, permeable to said analyte and interposed between said reagent layer and said spreading layer; the improvement comprising a radiation-transmissive, detectable species migration-inhibiting layer interposed between the reagent layer and the porous radiation-blocking layer, said detectable species migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said radiometrically detectable species to said porous radiation-blocking layer upon contact of said element with the liquid under analysis.
Claim 7
Dependent Claim
119 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a radiometrically detectable species, a spreading layer permeable to said analyte, and a porous radiation-blocking layer, permeable to said analyte and interposed between said reagent layer and said spreading layer; the improvement comprising a radiation-transmissive, detectable species migration-inhibiting layer interposed between the reagent layer and the porous radiation-blocking layer, said detectable species migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said radiometrically detectable species to said porous radiation-blocking layer upon contact of said element with the liquid under analysis.
Claim 8
Dependent Claim
102 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive support having thereon a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a dye, and an outermost radiation-blocking spreading layer permeable to said analyte; the improvement comprising a radiation-transmissive, dye migration-inhibiting layer interposed between the reagent layer and the radiation-blocking spreading layer, said dye migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said dye to said radiation-blocking spreading layer upon contact of said element with the liquid under analysis.
Claim 9
Dependent Claim
102 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive support having thereon a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a dye, and an outermost radiation-blocking spreading layer permeable to said analyte; the improvement comprising a radiation-transmissive, dye migration-inhibiting layer interposed between the reagent layer and the radiation-blocking spreading layer, said dye migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said dye to said radiation-blocking spreading layer upon contact of said element with the liquid under analysis.
Claim 10
Dependent Claim
24 words
An element as described in claim 5 which further comprises at least one radiation-transmissive registration layer interposed between said reagent layer and said support.
Claim 11
Dependent Claim
22 words
An element as described in claim 5 which further comprises a radiation-transmissive subbing layer interposed between said reagent layer and said support.
Claim 12
Dependent Claim
18 words
An element as described in claim 5 wherein the dye migration-inhibiting layer comprises a mordant for said dye.
Claim 13
Dependent Claim
76 words
An element as described in claim 5 wherein the dye migration-inhibiting layer comprises a mordant for the dye, said mordant having the structure ##STR5## each of R 1 , R 2 and R 3 , which may be the same or different, is selected from alkyl, alkenyl, aralkyl, or aryl having less than about eight carbon atoms; R 4 is a ballasting group having more than about 8 carbon atoms; and X.sup.⊖ is an acid anion.
Claim 14
Dependent Claim
153 words
An element as described in claim 5 wherein the dye migration-inhibiting layer comprises a mordant for the dye, said mordant being polymeric and containing a recurring unit having the structure ##STR6## wherein L is a linking group between Q and the atoms in the chain of the polymer backbone; n is 0 or 1; X.sup.⊖ is an acid anion; and Q.sup.⊕ is a linear or heterocyclic ammonium, phosphonium, or sulfur-containing group having one of the following structures: ##STR7## each of R 1 , R 2 , and R 3 , which may be the same or different, is selected from alkyl, alkenyl, aralkyl, or aryl having less than about 8 carbon atoms; each of R 5 , R 6 , R 7 , and R 8 , which may be the same or different, represent H or R 1 as defined above; and D is the atoms necessary to complete a heterocyclic ring.
Claim 15
Dependent Claim
16 words
An element as described in claim 5 wherein the dye migration-inhibiting layer comprises a hydrophilic colloid.
Claim 16
Dependent Claim
91 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive support having thereon a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a dye, and an outermost radiation-blocking spreading layer permeable to said analyte; the improvement comprising a radiation-transmissive, dye migration-inhibiting layer interposed between the reagent layer and the radiation-blocking spreading layer, said dye migration-inhibiting layer being permeable to said analyte and comprising a hydrophilic colloid and a mordant for said dye.
Claim 17
Dependent Claim
91 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive support having thereon a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a dye, and an outermost radiation-blocking spreading layer permeable to said analyte; the improvement comprising a radiation-transmissive, dye migration-inhibiting layer interposed between the reagent layer and the radiation-blocking spreading layer, said dye migration-inhibiting layer being permeable to said analyte and comprising a hydrophilic colloid and a mordant for said dye.
Claim 18
Dependent Claim
19 words
An element as described in claim 12 wherein the radiation-blocking spreading layer comprises a blushed polymer and a pigment.
Claim 19
Dependent Claim
21 words
An element as described in claim 12 wherein the reagent layer comprises a hydrophilic colloid having said interactive composition distributed therein.
Claim 20
Dependent Claim
108 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive support having thereon a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a hydrophilic colloid, said colloid having distributed therein a composition that is interactive in the presence of said analyte to provide a dye; and an outermost radiation-blocking spreading layer, permeable to said analyte, comprising a finely-divided particulate pigment and a blushed polymer; the improvement comprising a radiation-transmissive dye migration-inhibiting layer, interposed between the reagent layer and the radiation-blocking spreading layer, said dye migration-inhibiting layer being permeable to said analyte and comprising a mordant for said dye and a hydrophilic colloid.
Claim 21
Dependent Claim
108 words
In an element for the analysis of liquids, said element comprising a radiation-transmissive support having thereon a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a hydrophilic colloid, said colloid having distributed therein a composition that is interactive in the presence of said analyte to provide a dye; and an outermost radiation-blocking spreading layer, permeable to said analyte, comprising a finely-divided particulate pigment and a blushed polymer; the improvement comprising a radiation-transmissive dye migration-inhibiting layer, interposed between the reagent layer and the radiation-blocking spreading layer, said dye migration-inhibiting layer being permeable to said analyte and comprising a mordant for said dye and a hydrophilic colloid.
Claim 22
Dependent Claim
50 words
An element as described in claim 15 wherein the reagent layer is permeable to glucose and wherein the interactive composition in the reagent layer comprises glucose oxidase, peroxidase, and an indicator composition comprising a compound oxidizable in the presence of hydrogen peroxide and peroxidase to effect formation of said dye.
Claim 23
Dependent Claim
29 words
An element as described in claim 15 wherein the reagent layer is permeable to calcium and wherein the interactive composition in the reagent layer comprises an indicator for calcium.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-08-27 | 1983-02-26 | 1983-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-08-27 | 1987-02-26 | 1987-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-08-27 | 1991-02-26 | 1991-08-28 | $7700.00 | Window Closed / Lapsed |