US4393140A
Expired / Lapsed
US
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Process for the preparation of the highly pure enzyme kallikrein from swine pancreas extracts
Assignee: Bayer AG
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Filing: 1982-01-12
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Granted: 1983-07-12
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Expires: 2002-01-12
Abstract
Abstract The invention relates to a process for the preparation of the highly pure enzyme kallikrein from organ extracts, in particular from swine pancreas extracts, the accompanying proteolytic activity of the swine pancreas extract being separated off by chromatography on ion exchangers and the highly pure enzyme kallikrein being obtained by chromatography on support materials with a kallikrein-trypsin inhibitor (basic pancreatic trypsin inhibitor=BPTI) covalently bonded in a particular manner.
Inventors
- 👤 Hermann Schutt
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:40
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6445; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
113 words
A process for the preparation of pure kallikrein, which comprises adjusting an organ extract containing kallikrein to a conductivity of 2 to 6 mS cm -1 at a pH value of 5 to 8, bringing the organ extract thus prepared into contact with an ion exchanger in batch form or column form, eluting the kallikrein by continuously or discontinuously increasing the ionic strength by means of a mono- or divalent alkali metal salt or alkaline earth metal salt or by lowering the pH value and subsequently purifying the eluate by affinity chromatography on BPTI covalently bonded to a carrier and then desorbing from the BPTI-carrier at a pH value of 4.0 to 5.0.
Claim 2
Dependent Claim
113 words
A process for the preparation of pure kallikrein, which comprises adjusting an organ extract containing kallikrein to a conductivity of 2 to 6 mS cm -1 at a pH value of 5 to 8, bringing the organ extract thus prepared into contact with an ion exchanger in batch form or column form, eluting the kallikrein by continuously or discontinuously increasing the ionic strength by means of a mono- or divalent alkali metal salt or alkaline earth metal salt or by lowering the pH value and subsequently purifying the eluate by affinity chromatography on BPTI covalently bonded to a carrier and then desorbing from the BPTI-carrier at a pH value of 4.0 to 5.0.
Claim 3
Dependent Claim
28 words
A process according to claim 1, in which the ion exchanger used is based on cellulose, dextran or agarose and has been crosslinked by treatment with bifunctional reagents.
Claim 4
Dependent Claim
35 words
A process according to claim 1 or 2, in which the carrier to which the BPTI is covalent bonded is based on cellulose, dextran or agarose and has been crosslinked by treatment with bifunctional reagents.
Claim 5
Dependent Claim
21 words
A process according to claim 2, in which the bifunctional reagent is a halohydrin, bis-alkyl halide, bifunctional epoxide or divinyl compound.
Claim 6
Dependent Claim
20 words
A process according to claim 3, in which the bifunctional reagent in halohydrin, bis-alkyl halide, bifunctional epoxide or divinyl compound.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-07-11 | 1987-01-10 | 1987-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-07-11 | 1991-01-10 | 1991-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-07-11 | 1995-01-10 | 1995-07-12 | $7700.00 | Window Closed / Lapsed |