US4038141A
Expired / Lapsed
US
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Methods for extracting and purifying kallidinogenase
Assignee: Seikagaku Corp
•
Filing: 1976-04-07
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Granted: 1977-07-26
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Expires: 1996-04-07
Abstract
Abstract A method for extracting and collecting kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45° to about 60° C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidinogenase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.
Inventors
- 👤 Kiyochika Tokuyasu
- 👤 Takeshi Yokobori
- 👤 Chizuko Nakahara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 03:17
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6445; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
91 words
In a method for purifying kallidinogenase by subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion exchange resin, the improvement comprising (1) bringing said aqueous phase of the kallidinogenase-containing aqueous extract into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and (2) eluting the kallidinogenase with an aqueous solution of a salt consisting of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.
Claim 2
Dependent Claim
91 words
In a method for purifying kallidinogenase by subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion exchange resin, the improvement comprising (1) bringing said aqueous phase of the kallidinogenase-containing aqueous extract into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and (2) eluting the kallidinogenase with an aqueous solution of a salt consisting of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.
Claim 3
Dependent Claim
38 words
The method of claim 1 wherein said ammonium salt of a weak acid is selected from the group consisting of ammonium salts of weak inorganic acid and ammonium salts of fatty acids containing 1 to 5 carbon atoms.
Claim 4
Dependent Claim
49 words
The method of claim 2 wherien said ammonium salt is selected from the group consisting of ammonium carbonate, ammonium bicarbonate, ammonium borate, mono-or di-basic ammonium phosphate, ammonium phoshite, ammonium hypophosphite, ammonium formate, ammonium acetate, ammonium bimalate, ammonium citrate, ammonium lactate, ammonium oxalate, ammonium succinate, ammonium tartrate and ammonium valerate.
Claim 5
Dependent Claim
26 words
The method of claim 1 in which the adsorption step (1) is carried out at a pH of more than about 4 but below about 8.
Claim 6
Dependent Claim
24 words
The method of claim 1 in which the adsorption step (1) is carried out at a pH of from about 5 to about 7.
Claim 7
Dependent Claim
24 words
The method of claim 1 in which the adsorption step (1) is carried out at a pH of from about 5.5 to about 6.5.
Claim 8
Dependent Claim
24 words
The method of claim 1 in which the elution step (2) is carried out at a pH of from about 6 to about 7.
Claim 9
Dependent Claim
24 words
The method of claim 6 in which the elution step (2) is carried out at a pH of from about 6 to about 7.
Claim 10
Dependent Claim
23 words
The method of claim 1 in which the gel-type weakly basic anion-exchange resin comprises a resin having secondary or tertiary amine exchange groups.
Claim 11
Dependent Claim
23 words
The method of claim 8 in which the gel-type weakly basic anion-exchange resin comprises a resin having secondary or tertiary amine exchange groups.
Claim 12
Independent Claim
125 words
A method for extracting and collecting purified kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45° to about 60° C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid thereby obtaining an aqueous phase containing kallidinogenase, bringing said aqueous phase of the kallidinogenase-containing aqueous extract into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and eluting the kallidinogenase with an aqueous solution of a salt consisting of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.
Claim 13
Independent Claim
125 words
A method for extracting and collecting purified kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45° to about 60° C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid thereby obtaining an aqueous phase containing kallidinogenase, bringing said aqueous phase of the kallidinogenase-containing aqueous extract into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and eluting the kallidinogenase with an aqueous solution of a salt consisting of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.
References Cited (Backward Citations)
US3809748A
Cited
US3905870A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1980-07-25 | 1981-01-24 | 1981-07-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-07-25 | 1985-01-24 | 1985-07-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-07-25 | 1989-01-24 | 1989-07-26 | $7700.00 | Window Closed / Lapsed |