US3660237A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for obtaining kallekrein from pancreas or submandibularis glands of pigs
Assignee: Bayer AG
•
Filing: 1970-05-19
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Granted: 1972-05-02
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Expires: 1990-05-19
Abstract
Abstract The extraction of Kallekrein from the pancreas and submandibularis gland of pigs is carried out by subjecting the fresh comminuted glands at about room temperature with an aqueous solution of a salt, such as sodium chloride, of a concentration of from 3 to 25%, preferably 8% of the salt, at a pH value of 5.5 to 9.5, preferably 7.5 to 8.5, in the presence of a watermiscible solvent such as methanol, and the mixture stirred for about 1 hour. The aqueous kallekrein-containing phase is separated from the solids and the kallekrein precipitated in the presence of kieselguhr and acetone. High yields and great reduction in extraction time are noted.
Inventors
- 👤 Fritz Schultz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 22:11
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6445; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
21 words
A process as defined in claim 1 wherein the extraction is carried out in the presence of a water-miscible organic solvent.
Claim 2
Dependent Claim
26 words
A process as defined in claim 1 wherein the extraction is carried out at a pH from 7.5 to 8.5 and in the presence of methanol.
Claim 3
Dependent Claim
19 words
A process as defined in claim 3 wherein the aqueous salt solution is an 8% solution of common salt.
References Cited (Backward Citations)
US3260654A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-05-02 | 1975-11-01 | 1976-05-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-05-02 | 1979-11-01 | 1980-05-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-05-02 | 1983-11-01 | 1984-05-02 | $7700.00 | Window Closed / Lapsed |