US4421650A
Expired / Lapsed
US
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Process for separation of carbohydrates
Assignee: Seikagaku Corp
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Filing: 1983-01-26
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Granted: 1983-12-20
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Expires: 2003-01-26
Abstract
Abstract There is provided a process for the separation of carbohydrates by use of hydrophobic interaction chromatography. The process according to the present invention can accomplish effective separation of carbohydrates, particularly mucopolysaccharides including heparin, heparan sulfate, chondroitin sulfates, dermatan sulfate, hyaluronic acid, keratan sulfate and chondroitin-polysulfates. As the hydrophobic ligand in the chromatographic support to be used in the present invention, there may particularly be mentioned and alkyl group; an alkyl group substituted with a hydroxyl group, a carboxyl group and/or an amino group; an aryl group; or an aralkyl group. The chromatographic support is selected from cross-linked and non-cross-linked agaroses and polyvinyl alcohols.
Inventors
- 👤 Kinzo Nagasawa
- 👤 Akira Ogamo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 23:50
CPC Classifications
C; C08; C08B; C08B37/00; C08B37/006; C08B37/0063; C08B37/0075; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/32; B01D15/325; B01D15/327; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/287; C08B37/0003
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
98 words
A process for separation of mucopolysaccharides which comprises separating a mucopolysaccharide into its constituent components comprising contacting said mucopolysaccharide with a chromatographic column comprising a support selected from the group consisting of crosslinked agarose, non-cross-linked agarose, and a polyvinyl alcohol, said support containing hydrophobic ligands selected from the group consisting of alkyl groups; alkyl groups substituted with at least one group selected from the group consisting of a hydroxyl group, a carboxyl group and an amino group; alkylene groups substituted with a hydroxyl group; aryl groups; and alalkyl groups, and thereby separating said mucopolysaccharide into its constituent components.
Claim 2
Dependent Claim
98 words
A process for separation of mucopolysaccharides which comprises separating a mucopolysaccharide into its constituent components comprising contacting said mucopolysaccharide with a chromatographic column comprising a support selected from the group consisting of crosslinked agarose, non-cross-linked agarose, and a polyvinyl alcohol, said support containing hydrophobic ligands selected from the group consisting of alkyl groups; alkyl groups substituted with at least one group selected from the group consisting of a hydroxyl group, a carboxyl group and an amino group; alkylene groups substituted with a hydroxyl group; aryl groups; and alalkyl groups, and thereby separating said mucopolysaccharide into its constituent components.
Claim 3
Dependent Claim
23 words
The process according to claim 1, wherein the mucopolysaccharides are heparin, haparan sulfate, chondroitin sulfates, dermatan sulfate, hyaluronic acid, keratan sulfate, or chondroitin-polysulfates.
Claim 4
Dependent Claim
39 words
The process according to claim 1, wherein the alkyl group has 1 to 12 carbon atoms; the aryl group is a phenyl group or a naphthyl group; and the aralkyl group is a benzyl group or a phenethyl group.
Claim 5
Dependent Claim
51 words
The process according to any one of claims 2, 3, or 1, wherein the chromatographic support is selected from the group consisting of a crosslinked agarose, a phenyl agarose, an octyl agarose, a benzyl agarose, an ω-aminooctyl agarose, an ethyl agarose, a butyl agarose, a hexyl agarose and a decyl agarose.
Claim 6
Dependent Claim
14 words
The process according to claim 2, 3, or 1, wherein the mucopolysaccharide is heparin.
Claim 7
Dependent Claim
24 words
The process according to claim 2, 3 or 1, wherein the hydrophobic chromatography is carried out at a temperature of 0° to 50° C.
Claim 8
Dependent Claim
21 words
The process according to claim 6, wherein the hydrophobic chromatography is carried out at a temperature of 0° to 25° C.
Claim 9
Dependent Claim
11 words
The process according to claim 4, wherein said mucopolysaccharide is heparin.
Claim 10
Dependent Claim
11 words
The process according to claim 6, wherein said mucopolysaccharide is heparin.
Claim 11
Dependent Claim
21 words
The process according to claim 4, wherein the hydrophobic chromatography is carried out at a temperature of 0° to 25° C.
Claim 12
Dependent Claim
21 words
The process according to claim 8, wherein the hydrophobic chromatography is carried out at a temperature of 0° to 25° C.
Claim 13
Dependent Claim
21 words
The process according to claim 9, wherein the hydrophobic chromatography is carried out at a temperature of 0° to 25° C.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-12-19 | 1987-06-20 | 1987-12-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-12-19 | 1991-06-20 | 1991-12-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-12-19 | 1995-06-20 | 1995-12-20 | $7700.00 | Window Closed / Lapsed |