US4319975A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Derivatized agarose and method of making and using same
Assignee: FMC Corp
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Filing: 1980-10-20
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Granted: 1982-03-16
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Expires: 2000-10-20
Abstract
Abstract Derivatized agarose is produced by introducing into the disaccharide unit of the agarose polymer chain, at least one substituent having a molecular weight range greater than 100 to about 1,000,000. The derivatized agarose is useful as a sieving gel in electrophoresis and diffusive interactions.
Inventors
- 👤 Richard B. Cook
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 11:52
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; C; C08; C08B; C08B37/00; C08B37/0006; C08B37/0036; C08B37/0039
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
71 words
A method of separating biological mixtures by subjecting them to gel electrophoresis using as the gel matrix a derivatized agarose containing at least one substituent having a preselected conformational shape such that the pore diameter of the derivatized agarose is not reduced below about 10° A units, the D.S. being from about 0.001 to about 2.0, whereby the components in said mixture are separated as a function of their molecular size.
Claim 2
Independent Claim
71 words
A method of separating biological mixtures by subjecting them to gel electrophoresis using as the gel matrix a derivatized agarose containing at least one substituent having a preselected conformational shape such that the pore diameter of the derivatized agarose is not reduced below about 10° A units, the D.S. being from about 0.001 to about 2.0, whereby the components in said mixture are separated as a function of their molecular size.
Claim 3
Dependent Claim
11 words
The method according to claim 1 wherein the substituent is 2-hydroxyethyl.
Claim 4
Dependent Claim
13 words
The method according to claim 1 wherein the biological mixtures are DNA fragments.
Claim 5
Dependent Claim
12 words
The method according to claim 1 wherein the biological mixtures are proteins.
Claim 6
Dependent Claim
57 words
A derivatized agarose, useful as an electrophoretic sieving gel, containing at least one substituent having a molecular weight range greater than 100 to about 1,000,000 and a preselected conformational shape such that the average pore diameter of the derivatized agarose is not reduced below about 10° A units, the D.S. being from about 0.001 to about 2.0.
Claim 7
Dependent Claim
57 words
A derivatized agarose, useful as an electrophoretic sieving gel, containing at least one substituent having a molecular weight range greater than 100 to about 1,000,000 and a preselected conformational shape such that the average pore diameter of the derivatized agarose is not reduced below about 10° A units, the D.S. being from about 0.001 to about 2.0.
Claim 8
Dependent Claim
33 words
The derivatized agarose according to claim 5 wherein the substituent is attached to the agarose molecule via a linkage selected from the class consisting of ether, ester, amide, amine, isourea, and carbamate linkages.
Claim 9
Dependent Claim
20 words
The derivatized agarose according to claim 6 wherein the substituent is attached to the agarose molecule via an ether linkage.
Claim 10
Dependent Claim
20 words
The derivatized agarose according to claim 6 wherein the substituent is attached to the agarose molecule via an ester linkage.
Claim 11
Dependent Claim
20 words
The derivatized agarose according to claim 6 wherein the substituent is attached to the agarose molecule via an amide linkage.
Claim 12
Dependent Claim
20 words
The derivatized agarose according to claim 6 wherein the substituent is attached to the agarose molecule via an amine linkage.
Claim 13
Dependent Claim
20 words
The derivatized agarose according to claim 6 wherein the substituent is attached to the agarose molecule via an isourea linkage.
Claim 14
Dependent Claim
20 words
The derivatized agarose according to claim 6 wherein the substituent is attached to the agarose molecule via a carbamate linkage.
References Cited (Backward Citations)
US3527712A
Cited
US3959251A
Cited
US3956273A
Cited
US3956273B1
Cited
US3956272A
Cited
US4055510A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-03-15 | 1985-09-14 | 1986-03-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-03-15 | 1989-09-14 | 1990-03-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-03-15 | 1993-09-14 | 1994-03-16 | $7700.00 | Window Closed / Lapsed |