US5268097A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Passivated and stabilized porous mineral oxide supports and method for the preparation and use of same
Assignee: Life Technologies Inc
•
Filing: 1992-10-05
•
Granted: 1993-12-07
•
Expires: 2012-10-05
Abstract
Abstract This invention relates generally to modified porous solid supports and processes for the preparation and use of same. In particular, passivated porous mineral oxide supports are disclosed that are stabilized against leaching under harsh environmental conditions by the application of a thin, protective polymeric coating atop their porous surfaces and that are characterized by a reversible high sorptive capacity substantially unaccompanied by non-specific adsorption of or interaction with biomolecules. Passivation is achieved by the use of a passivation mixture comprising a main monomer, a passivating monomer that is different from the main monomer, and a crosslinking agent, which mixture upon polymerization results in the substantial elimination of the undesirable non-specific interaction between biomolecules and the protective polymeric coating on the surfaces of the mineral oxide matrix.
Inventors
- 👤 Pierre Girot
- 👤 Egisto Boschetti
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 21:54
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/10; B01D15/20; B01D15/206; B01J; B01J20/00; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28054; B01J20/28057; B01J20/28069; B01J20/28078; B01J20/281; B01J20/286; B01J20/288; B01J20/30; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3206; B01J20/3208; B01J20/321; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/327; B01J20/328; B01J20/3282; B01J20/3289; B01J39/00; B01J39/08; B01J39/16; B01J39/17; B01J39/26; B01J41/00; B01J41/20; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58
Claims Analysis
Total Claims: 40
Claim 1
Independent Claim
111 words
A passivated porous support comprising (i) a porous mineral oxide matrix having interior and exterior surfaces substantially covered by a thin, protective polymer surface coating having innate hydrophobic groups that render said coating susceptible to undesirable non-specific interaction with one or more biological molecules, and (ii) a polymer network derived from a passivation mixture comprising a main monomer, a passivating monomer different from said main monomer, and a crosslinking agent, said mixture having been allowed to come into intimate contact with said surfaces of said coating such that on polymerization of said mixture said innate groups of said coating become deactivated, resulting in the substantial elimination of said undesirable non-specific interaction.
Claim 2
Dependent Claim
111 words
A passivated porous support comprising (i) a porous mineral oxide matrix having interior and exterior surfaces substantially covered by a thin, protective polymer surface coating having innate hydrophobic groups that render said coating susceptible to undesirable non-specific interaction with one or more biological molecules, and (ii) a polymer network derived from a passivation mixture comprising a main monomer, a passivating monomer different from said main monomer, and a crosslinking agent, said mixture having been allowed to come into intimate contact with said surfaces of said coating such that on polymerization of said mixture said innate groups of said coating become deactivated, resulting in the substantial elimination of said undesirable non-specific interaction.
Claim 3
Dependent Claim
15 words
The passivated porous support of claim 1 wherein said support has reversible high sorptive capacity.
Claim 4
Dependent Claim
33 words
The passivated porous support of claim 2 in which said reversible sorptive capacity for one of said biological molecules ranges from about 1 to about 300 milligrams per milliter of passivated porous support.
Claim 5
Dependent Claim
21 words
The passivated porous support of claim 1 wherein said support has chemical stability on exposure to strongly acidic or alkaline medium.
Claim 6
Dependent Claim
19 words
The passivated porous support of claim 1 wherein said support has chemical stability on exposure to strongly oxidizing medium.
Claim 7
Dependent Claim
53 words
The passivated porous support of claim 1 in which said coating comprises a linear polymer capable of being dissolved in a suitable solvent to form a coating solution, said linear polymer being selected from the group consisting of polystyrene, polysulfone, polyethersulfone, cellulose acetate, cellulose nitrate, polyvinylacetate, polyacrylates, polyvinylidine fluoride, polyacrylonitrile, polyamides, and polyimides.
Claim 8
Dependent Claim
14 words
The passivated porous support of claim 1 in which said polymeric coating comprises polystyrene.
Claim 9
Dependent Claim
25 words
The passivated porous support of claim 1 in which said matrix has an initial average particle size ranging from about 5 to about 1000 microns.
Claim 10
Dependent Claim
25 words
The passivated porous support of claim 1 in which said matrix has an initial average particle size ranging from about 10 to about 100 microns.
Claim 11
Dependent Claim
26 words
The passivated porous support of claim 1 in which said matrix has an initial porous volume ranging from about 0.2 to about 2 cm 3 /gram.
Claim 12
Dependent Claim
26 words
The passivated porous support of claim 1 in which said matrix has an initial surface area ranging from about 1 to about 800 m 2 /gram.
Claim 13
Dependent Claim
24 words
The passivated porous support of claim 1 in which said matrix has an initial pore size ranging from about 50 to about 6000 Angstroms.
Claim 14
Dependent Claim
24 words
The passivated porous support of claim 1 which wherein said support has a size exclusion limit ranging from about 500 to about 2,000,000 daltons.
Claim 15
Dependent Claim
24 words
The passivated porous support of claim 1 in which said polymerization of said passivation mixture is effected in the presence of a pore inducer.
Claim 16
Dependent Claim
27 words
The passivated porous support of claim 14 in which said pore inducer is selected from the group consisting of a polyethylene glycol, a polyoxyethylene, and a polysaccharide.
Claim 17
Dependent Claim
25 words
The passivated porous support of claim 1 in which said polymerization of said passivation mixture is effected in the presence of a polar organic solvent.
Claim 18
Dependent Claim
31 words
The passivated porous support of claim 16 in which said polar organic solvent is selected from the group consisting of methanol, ethanol, propanol, tetrahydrofuran, dimethylsulfoxide, dimethylformamide, acetone, dioxane, and mixtures thereof.
Claim 19
Dependent Claim
24 words
The passivated porous support of claim 1 in which said polymerization of said passivation mixture is effected in the presence of a polymerization initiator.
Claim 20
Dependent Claim
26 words
The passivated porous support of claim 18 in which said polymerization initiator is selected from the group consisting of organic-soluble tertiary amines, nitriles, and photochemical initiators.
Claim 21
Dependent Claim
14 words
The passivated porous support of claim 18 in which said polymerization initiator is azo-bis-amidinopropane.
Claim 22
Dependent Claim
20 words
The passivated porous support of claim I8 in which said polymerization of said passivation mixture is effected by thermal energy.
Claim 23
Dependent Claim
22 words
The passivated porous support of claim 1 in which said main monomer comprises a vinyl monomer having at least one polar substituent.
Claim 24
Dependent Claim
14 words
The passivated porous support of claim 22 in which said polar substituent is nonionic.
Claim 25
Dependent Claim
16 words
The passivated porous support of claim 22 in which said polar substituent is ionic or ionizable.
Claim 26
Dependent Claim
28 words
The passivated porous support of claim 22 in which said vinyl monomer has at least two polar substituents that may be ionic, nonionic, ionizable or a combination thereof.
Claim 27
Dependent Claim
15 words
The passivated porous support of claim 22 in which said polar substituent is positively charged.
Claim 28
Dependent Claim
15 words
The passivated porous support of claim 22 in which said polar substituent is negatively charged.
Claim 29
Dependent Claim
28 words
The passivated porous support of claim 1 in which said main monomer is selected to provide a polymer network that has an affinity for a preselected biological molecule.
Claim 30
Dependent Claim
22 words
The passivated porous support of claim 1 in which said passivating monomer comprises a vinyl monomer having at least one hydrophobic substituent.
Claim 31
Dependent Claim
44 words
The passivated porous support of claim 29 in which said hydrophobic substituent on said passivating monomer contains from about 1 to about 20 carbon atoms and is selected from the group consisting of straight-chain alkyl groups, branched-chain alkyl groups, aromatic groups, and arylaromatic groups.
Claim 32
Dependent Claim
22 words
The passivated porous support of claim 1 in which said passivating monomer is selected to deactivate hydrophobic groups on said surface coating.
Claim 33
Dependent Claim
24 words
The passivated porous support of claim 1 in which said passivating monomer is selected from the group consisting of N-alkylacrylamides, N-arylacrylamides, and derivatives thereof.
Claim 34
Dependent Claim
29 words
The passivated porous support of claim 1 in which said passivating monomer is selected from the group consisting of N-tert-octylacrylamide, N-(1-methylundecyl)acrylamide, N-(1,1,3,5-tetramethyl)octylacrylamide, a methacrylate of octylphenoxy polyethoxyethanol, and polyethyleneglycol-dimethacrylate.
Claim 35
Dependent Claim
23 words
The passivated porous support of claim 1 in which said crosslinking agent comprises a vinyl monomer having at least one other polymerizable group.
Claim 36
Dependent Claim
37 words
The passivated porous support of claim 34 in which said polymerizable group is selected from the group consisting of a double bond, a triple bond, an allylic group, an epoxide, an azetidine, or a strained carbocyclic ring.
Claim 37
Dependent Claim
39 words
The passivated porous support of claim 1 in which said crosslinking agent is selected from the group consisting of N,N'-methylenebis(acrylamide), N,N'-methylenebis(methacrylamide), diallyl tartradiamide, allyl methacrylate, diallyl amine, diallyl ether, diallyl carbonate, divinyl ether, 1,4-butanediol-divinylether, polyethyleneglycol divinyl ether, or 1,3-diallyloxy-2-propanol.
Claim 38
Dependent Claim
34 words
The passivated porous support of claim 1 in which said innate hydrophobic groups present in said polymer surface coating can participate in hydrophobic-hydrophobic bonding interactions with a hydrophobic substituent present in said passivating monomer.
Claim 39
Dependent Claim
119 words
A passivated porous support comprising (i) a porous silica matrix having interior and exterior surfaces substantially covered by a thin, protective polystyrene surface coating having innate hydrophobic groups that render said coating susceptible to undesirable non-specific interactions with one or more biological molecules, and (ii) a polymer network derived from a passivation mixture comprising a main monomer, a passivating monomer different from said main monomer, and a crosslinking agent, said mixture having been allowed to come into intimate contact with said surfaces of said coating for a sufficient period of time such that on polymerization of said mixture said hydrophobic groups of said coating become substantially covered and deactivated, resulting in the substantial elimination of said undesirable non-specific interaction.
Claim 40
Dependent Claim
119 words
A passivated porous support comprising (i) a porous silica matrix having interior and exterior surfaces substantially covered by a thin, protective polystyrene surface coating having innate hydrophobic groups that render said coating susceptible to undesirable non-specific interactions with one or more biological molecules, and (ii) a polymer network derived from a passivation mixture comprising a main monomer, a passivating monomer different from said main monomer, and a crosslinking agent, said mixture having been allowed to come into intimate contact with said surfaces of said coating for a sufficient period of time such that on polymerization of said mixture said hydrophobic groups of said coating become substantially covered and deactivated, resulting in the substantial elimination of said undesirable non-specific interaction.
References Cited (Backward Citations)
JPS50115298A
Cited
US4045353A
Cited
JPS5174694A
Cited
US4673734A
Cited
US4140653A
Cited
JPS5254496A
Cited
US4335017A
Cited
US4385991A
Cited
SU747513A1
Cited
US4189370A
Cited
US4229536A
Cited
US4352884A
Cited
US4415631A
Cited
US4443339A
Cited
US4460625A
Cited
FR2541593A1
Cited
US4627992A
Cited
US4729834A
Cited
JPS6174644A
Cited
US4618533A
Cited
US4761232A
Cited
JPS62286533A
Cited
US4882048A
Cited
US4882226A
Cited
US4917793A
Cited
US5053135A
Cited
US5114577A
Cited
US5015373A
Cited
US4931498A
Cited
US5047438A
Cited
US4933372A
Cited
US5045190A
Cited
US4957620A
Cited
US5135650A
Cited
US5091433A
Cited
US5030352A
Cited
US5182016A
Cited
US5104729A
Cited
US5160627A
Cited
US5066398A
Cited
US5137633A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-12-06 | 1997-06-07 | 1997-12-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-12-06 | 2001-06-07 | 2001-12-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-12-06 | 2005-06-07 | 2005-12-07 | $7700.00 | Window Closed / Lapsed |