Composite separating agent

Claim 1

A composite separating agent for aqueous gel permeation chromatography which comprises a crosslinked polysaccharide hydrophilic polymer separating agent incorporated within pores of an organic polymer substrate; wherein said separating agent shows a degree of swelling in water of 10 to 100 ml/g-dry, and has a macro-network structure, each pore of said substrate having a pore volume of 0.5 to 3 ml/g and a pore radius of 200 to 100,000 Å; and said substrate shows a degree of swelling in water of 20 ml/g-dry or below and a degree of crosslinking of 4 to 100 mol %, has a porous structure, and is a copolymer of a monounsaturated monomer and a polyunsaturated monomer; wherein said monounsaturated monomer is selected from the group consisting of styrene, ethylvinylbenzene, p-methyl styrene, glycidyl methacrylate, allyl glycidyl ether, acryl glycidyl ether, 2-hydroxyethyl methacrylate, polyethyleneglycol methacrylate, methacrylic acid, methyl methacrylate, vinyl formate, vinyl acetate, allyl formate, and allyl acetate; and said polyunsaturated monomer is selected from the group consisting of divinyl benzene, trivinyl benzene, and substituted derivatives thereof, ethylene glycol dimethacrylate, polyethylene glycol dimethacrylate, propylene glycol dimethacrylate, polypropylene glycol dimethacrylate, glycerol trimethacrylate, glycerol dimethacrylate, and triallyl isocyanurate.