US4447328A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Filler for liquid chromatography and method for separating water-soluble substances using said filler
Assignee: Sekisui Chemical Co Ltd
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Filing: 1982-02-16
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Granted: 1984-05-08
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Expires: 2002-02-16
Abstract
Abstract A liquid-chromatographic filler comprising a particulate synthetic polymer, such as glycerol di or tri (meth) acrylate wherein the filler exhibits a behavior such that in chromatography using polyethylene glycol as a sample and water as an eluent, the elution time of the sample increases with a rise in temperature. Separation of water-soluble substances by liquid chromatography can be achieved by using an aqueous medium as an eluent, and this filler.
Inventors
- 👤 Fumio Kamiyama
- 👤 Kazutoshi Yamazaki
- 👤 Minoru Kohara
- 👤 Mituo Nagata
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 19:58
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01J; B01J20/00; B01J20/22; B01J20/26; B01J20/262; B01J20/281; B01J20/282; B01J20/285; B01J20/286; B01J20/287; B01J2220/00; B01J2220/50; B01J2220/54; G; G01; G01N; G01N30/00; G01N30/02; G01N30/50; G01N30/52; G01N30/54
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
155 words
A method for separating water-soluble substances, which comprises subjecting said water-soluble substances to reversed phase liquid chromatography using an aqueous medium as an eluent and a filler comprising a particulate synthetic polymer, said polymer being a single polymer of (a) a monomer selected from the group consisting of tetramethylolmethane di-, tri- or tetra-(meth)acrylate, trimethylolpropane tri-(meth)acrylate, trimethylolethane tri-(meth) acrylate, dipentaerythritol di- to hexa-(meth)acrylate, glycerol di- or tri-(meth)acrylate, polyethylene glycol di-(meth)acrylate of the formula ##STR16## wherein R 1 is H or CH 3 , and 3≧n≧1, polypropylene glycol di-(meth)acrylate of the formula ##STR17## wherein R 1 is H or CH 3 , and n is an integer of 1-24, and neopentyl glycol di(meth)acrylate, or (b) a combination of two or more of said monomers, said filler exhibiting a behavior such that in liquid chromatography using polyethylene glycol as a sample and water as an eluent, the elution time of the sample increases with a rise in temperature.
Claim 2
Independent Claim
155 words
A method for separating water-soluble substances, which comprises subjecting said water-soluble substances to reversed phase liquid chromatography using an aqueous medium as an eluent and a filler comprising a particulate synthetic polymer, said polymer being a single polymer of (a) a monomer selected from the group consisting of tetramethylolmethane di-, tri- or tetra-(meth)acrylate, trimethylolpropane tri-(meth)acrylate, trimethylolethane tri-(meth) acrylate, dipentaerythritol di- to hexa-(meth)acrylate, glycerol di- or tri-(meth)acrylate, polyethylene glycol di-(meth)acrylate of the formula ##STR16## wherein R 1 is H or CH 3 , and 3≧n≧1, polypropylene glycol di-(meth)acrylate of the formula ##STR17## wherein R 1 is H or CH 3 , and n is an integer of 1-24, and neopentyl glycol di(meth)acrylate, or (b) a combination of two or more of said monomers, said filler exhibiting a behavior such that in liquid chromatography using polyethylene glycol as a sample and water as an eluent, the elution time of the sample increases with a rise in temperature.
Claim 3
Dependent Claim
18 words
The method of claim 1 wherein the pH of the aqueous medium as eluent is varied during chromatography.
Claim 4
Dependent Claim
20 words
The method of claim 1 wherein the pH of the aqueous medium as eluent is increased as the chromatography proceeds.
Claim 5
Dependent Claim
13 words
The method of claim 1 wherein said water-soluble substances are hydrophilic biochemical substances.
Claim 6
Dependent Claim
16 words
The method of claim 1 wherein said water-soluble substances are proteins, enzymes, vitamins or amino acids.
References Cited (Backward Citations)
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US3657117A
Cited
US3960720A
Cited
JPS501760A
Cited
US4140653A
Cited
US4135892A
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US4189370A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-08 | 1987-11-07 | 1988-05-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-08 | 1991-11-07 | 1992-05-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-08 | 1995-11-07 | 1996-05-08 | $7700.00 | Window Closed / Lapsed |