US4189370A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for obtaining gels of N-methylol-acrylamide copolymers and application of said gels for the stepped gradient separation of seric lipoproteins
Assignee: Sebia SA
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Filing: 1978-06-21
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Granted: 1980-02-19
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Expires: 1998-06-21
Abstract
Abstract A process for preparing plates of a new gel polymer, said plates being adapted to the electrophoresis separation of the seric or plasmatic lipoproteins under a stepped gradient. The gel polymers are prepared by radical polymerization of N-methylol-acrylamide and of a bifunctional allylic or acrylic compound causing cross-linking to yield a tridimensional configuration polymer. The polymerization may optionally be catalyzed by peroxides and/or ultraviolet radiation. Anionic polysaccharides containing only COOH groups may be added to assist migration of lipoproteins in the gel.
Inventors
- 👤 Egisto Boschetti
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 02:07
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
120 words
A process for preparing gels stratified upon a film, said gels being adapted for electrophoretic separation of seric and plasmatic lipoproteins, said gel further being a high polymer of N-Methylol-Acrylamide, said process comprising the steps of: (a) disposing upon said film a first gel comprising a high polymer of N-Methylol-Acrylamide, said first gel being formed from a first monomer concentration; and (b) disposing upon said film a second gel comprising a high polymer of N-Methylol-Acrylamide said second gel being formed from a second monomer concentration different than said first monomer concentration, so as to form a discontinuous gradient between said first gel and said second gel; whereby electrophoretic separation of seric and plasmatic lipoproteins is enhanced by said discontinuous gradient.
Claim 2
Dependent Claim
120 words
A process for preparing gels stratified upon a film, said gels being adapted for electrophoretic separation of seric and plasmatic lipoproteins, said gel further being a high polymer of N-Methylol-Acrylamide, said process comprising the steps of: (a) disposing upon said film a first gel comprising a high polymer of N-Methylol-Acrylamide, said first gel being formed from a first monomer concentration; and (b) disposing upon said film a second gel comprising a high polymer of N-Methylol-Acrylamide said second gel being formed from a second monomer concentration different than said first monomer concentration, so as to form a discontinuous gradient between said first gel and said second gel; whereby electrophoretic separation of seric and plasmatic lipoproteins is enhanced by said discontinuous gradient.
Claim 3
Dependent Claim
22 words
A process according to claim 1, wherein said high polymer of N-Methylol-acrylamide is further formed from a jellifiable polysaccharide containing carboxyllic groups.
Claim 4
Dependent Claim
20 words
Process according to claim 1 said high polymer is obtained through copolymerizing N-methylolacrylamide with a bifunctional allylic or acrylic compound.
Claim 5
Dependent Claim
16 words
Process according to claim 3, wherein, to obtain an insoluble gel, said bifunctional compound is NN'-methylene-bis-acrylamide.
Claim 6
Dependent Claim
24 words
Process according to claim 3, wherein to obtain a gel soluble in a 2% aqeuous solution of periodic acid, said bifunctional compound is diallyltartramide.
Claim 7
Dependent Claim
19 words
Process according to claim 3, wherein to obtain a gel soluble in alkali solutions, said bifunctional compound is ethylene-diacrylate.
Claim 8
Dependent Claim
32 words
Process according to claim 1, wherein said first gel forms a first layer and said second gel forms a second layer spaced from said polyester film and disposed on said first layer.
Claim 9
Dependent Claim
53 words
Process according to claim 3, wherein said first monomer concentration is about 2.5%, said second monomer concentration is about 2%, the concentration of said bifunctional allylic or acrylic compound is about 7.5% for said first gel, and the concentration of said bifunctional allylic or acrylic compounds is about 10-17% for said second gel.
Claim 10
Dependent Claim
20 words
Process according to claim 2, wherein said polysaccharide is a gel of strongly electro endosmatic agarose containing only carboxylic groups.
Claim 11
Dependent Claim
15 words
Process according to claim 9, wherein the concentration of said polysaccharide is 0.1 to 1%.
Claim 12
Dependent Claim
16 words
Process according to claim 1, wherein said film used as support comprises an activated hydrophobic face.
Claim 13
Dependent Claim
23 words
Process according to claim 1, wherein said first gel and said second gel further contain agents enabling the rehydration of the dried gel.
Claim 14
Dependent Claim
23 words
Process according to claim 12, wherein said agents are previously treated, then included by washing into said first gel and said second gel.
Claim 15
Dependent Claim
28 words
Process according to claim 13, wherein said agents are polysaccharides of low molecular weight having a viscosity less than 30 cp in a 1.2% solution at 20° C.
Claim 16
Dependent Claim
22 words
Process according to claim 14, wherein said polysaccharides are negatively charged through carboxylic groups under the form of sodium or ammonium salts.
Claim 17
Dependent Claim
28 words
Process according to claim 12, wherein said agents are a member selected from the group consisting of sorbitol, ethylene glycol, and glycerol, used at a concentration of 1-10%.
Claim 18
Dependent Claim
11 words
Process according to claim 13, wherein said treatment is a reduction.
Claim 19
Dependent Claim
14 words
Process according to claim 17, wherein said reduction is carried out through inorganic hydrides.
Claim 20
Dependent Claim
24 words
Process according to claim 16, wherein said hydrides are a member selected from the group consisting of sodium borohydride, potassium borohydride, and aluminum-lithium hydride.
Claim 21
Dependent Claim
110 words
A plate adapted for electrophoretic separation of seric and plasmatic lipoproteins, said plate comprising: a film suitable for support of electrophoresis gels; a first gel disposed upon said film, said first gel comprising a high polymer of N-Methylol-Acrylamide, said first gel being formed from a first monomer concentration; and a second gel disposed upon said film so as to form a separation line between said first gel and said second gel, said second gel comprising a high polymer of N-Methylol-Acrylamide, said second gel being formed from a second monomer concentration different than said first monomer concentration, whereby electrophoretic separation of seric and plasmatic lipoproteins is enhanced by said separation line.
Claim 22
Dependent Claim
110 words
A plate adapted for electrophoretic separation of seric and plasmatic lipoproteins, said plate comprising: a film suitable for support of electrophoresis gels; a first gel disposed upon said film, said first gel comprising a high polymer of N-Methylol-Acrylamide, said first gel being formed from a first monomer concentration; and a second gel disposed upon said film so as to form a separation line between said first gel and said second gel, said second gel comprising a high polymer of N-Methylol-Acrylamide, said second gel being formed from a second monomer concentration different than said first monomer concentration, whereby electrophoretic separation of seric and plasmatic lipoproteins is enhanced by said separation line.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-02-18 | 1983-08-20 | 1984-02-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-02-18 | 1987-08-20 | 1988-02-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-02-18 | 1991-08-20 | 1992-02-19 | $7700.00 | Window Closed / Lapsed |