US4352884A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Carrier having acrylate copolymer coating for immobilization of bioactive materials
Assignee: Kuraray Co Ltd
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Filing: 1981-02-09
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Granted: 1982-10-05
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Expires: 2001-02-09
Abstract
Abstract Carriers coated with a copolymer of a hydrophilic acrylate or methacrylate monomer and a copolymerizable unsaturated carboxylic acid or unsaturated amine are provided which are substantially free from nonspecific adsorption of proteins and the like. Due to the side-chain carboxyl or amino groups of the copolymer, these coated carriers are able to immobilize bio-active materials such as antigens, antibodies, complements and enzymes. These carriers, on which bio-active materials can be immobilized, are useful as clinical selective adsorbents, affinity-chromatographic adsorbents, selective electrodes or columns for analysis.
Inventors
- 👤 Toshihide Nakashima
- 👤 Masao Tanihara
- 👤 Koichi Takakura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:24
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/30; B01J20/32; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/3276; B01J20/281; B01J20/286; B01J20/3202; B01J20/3272; B01J20/3274; B01J20/328; B01J20/3282; C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01J2220/00; B01J2220/50; B01J2220/54; Y; Y10; Y10S; Y10S435/00; Y10S435/962
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
194 words
A carrier for immobilization of bio-active materials which comprises a base material coated with a copolymer of (i) a hydrophilic acrylate or methacrylate monomer of the general formula CH 2 =(R 1 )CO 2 R 2 OR 3 , wherein R 1 is H or methyl; R 2 is a substituted or unsubstituted divalent alkylene radical of 2 to 3 carbon atoms or a poly(oxyalkylene) radical; R 3 is H or an alkyl radical of 1 to 3 carbon atoms, which alkyl radical can be further substituted by polar substituent groups and (ii) a copolymerizable unsaturated carboxylic acid of the general formula CH 2 =C(R 1 )CO 2 H, wherein R 1 is H or methyl or (iii) a copolymerizable unsaturated amine of the general formula CH 2 =C(R 1 )CO 2 R 2 NHR 3 , wherein R 1 is H or methyl; R 2 is a divalent alkylene radical of 2 or 3 carbon atoms; and R 3 is H or an alkyl radical of 1 to 3 carbon atoms, said copolymerizable component being present in amount of from about 1 to 50 weight percent based on the total weight of monomers.
Claim 2
Dependent Claim
194 words
A carrier for immobilization of bio-active materials which comprises a base material coated with a copolymer of (i) a hydrophilic acrylate or methacrylate monomer of the general formula CH 2 =(R 1 )CO 2 R 2 OR 3 , wherein R 1 is H or methyl; R 2 is a substituted or unsubstituted divalent alkylene radical of 2 to 3 carbon atoms or a poly(oxyalkylene) radical; R 3 is H or an alkyl radical of 1 to 3 carbon atoms, which alkyl radical can be further substituted by polar substituent groups and (ii) a copolymerizable unsaturated carboxylic acid of the general formula CH 2 =C(R 1 )CO 2 H, wherein R 1 is H or methyl or (iii) a copolymerizable unsaturated amine of the general formula CH 2 =C(R 1 )CO 2 R 2 NHR 3 , wherein R 1 is H or methyl; R 2 is a divalent alkylene radical of 2 or 3 carbon atoms; and R 3 is H or an alkyl radical of 1 to 3 carbon atoms, said copolymerizable component being present in amount of from about 1 to 50 weight percent based on the total weight of monomers.
Claim 3
Dependent Claim
52 words
A carrier for immobilization of bio-active materials as claimed in claim 1 wherein the base material is a member selected from the group consisting of glass, activated carbon, silica, alumina and high molecular weight organic compounds and said base material is employed in the form of grains, beads, webs, sheets or tubes.
Claim 4
Dependent Claim
31 words
A carrier for immobilization of bio-active materials as claimed in claim 2 wherein the base material is a porous or nonporous glass with a particle diameter from 0.05 to 5 millimeters.
Claim 5
Dependent Claim
34 words
A carrier for immobilization of bio-active materials as claimed in claim 1 wherein the copolymer additionally contains a sufficient amount of an epoxy-containing monomer to effect cross-linking of the copolymer coating upon subsequent curing.
Claim 6
Dependent Claim
31 words
A carrier for immobilization of bio-active materials as claimed in claim 1 wherein the copolymerizable component is present in the copolymer in amounts of from about 10 to 40 weight percent.
Claim 7
Dependent Claim
22 words
A clinical selective adsorbent comprising a bio-active material immobilized on a base material coated with a copolymer as claimed in claim 1.
Claim 8
Dependent Claim
28 words
A clinical selective adsorbent as claimed in claim 6 wherein the base material is a porous or nonporous glass with a particle diameter of 0.05 to 5 millimeters.
Claim 9
Dependent Claim
28 words
A clinical selective adsorbent as claimed in claim 6 wherein the bio-active material is a member selected from the group consisting of antigens, antibodies, complements, receptors and enzymes.
Claim 10
Dependent Claim
35 words
A clinical selective adsorbent as claimed in claim 6 wherein the bio-active material is a member selected from the group consisting of protein A, cell wall Fc receptor, complement Cl component, anti-immunoglobulin antibody and immunoglobulin.
Claim 11
Dependent Claim
27 words
An affinity chromatographic adsorbent comprising an antigen, antibody, complement or enzyme immobilized on a particulate or tubular base material coated with a copolymer as in claim 1.
Claim 12
Dependent Claim
32 words
An affinity chromatographic adsorbent as claimed in claim 10 wherein said particulate material comprises beads of glass or high molecular weight organic compounds having a particle size of 0.05 to 5 millimeters.
Claim 13
Dependent Claim
22 words
An affinity chromatographic adsorbent as claimed in claim 10 wherein said tubular material is a tube of glass or an organic polymer.
Claim 14
Dependent Claim
30 words
A selective electrode coated on the surface thereof with a copolymer as claimed in claim 1 and carrying an antigen, antibody, complement or enzyme immobilized on the surface so coated.
Claim 15
Dependent Claim
49 words
A column for analysis, a portion or the whole of which, comprises a surface formed of beads or a tube of glass or an organic polymer coated with a copolymer as claimed in claim 1 and carrying an antigen, antibody, complement or enzyme immobilized on the surface so coated.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-10-04 | 1986-04-05 | 1986-10-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-10-04 | 1990-04-05 | 1990-10-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-10-04 | 1994-04-05 | 1994-10-05 | $7700.00 | Window Closed / Lapsed |