US4206259A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Support matrices for immobilized enzymes
Assignee: Honeywell UOP LLC
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Filing: 1978-10-16
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Granted: 1980-06-03
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Expires: 1998-10-16
Abstract
Abstract Organic-inorganic support matrices for immobilized enzymes comprise a solid, porous, inorganic, water insoluble support combined with a copolymeric material resulting from the reaction of aminopolystyrene and a bifunctional monomer. The matrix is prepared by depositing a salt of amino-polystyrene within the pores of the solid support from an aqueous solution at a pH less than 7 after which the resulting composite is then reacted with an excess of a bifunctional reactive monomer thus forming a copolymeric organic material in situ substantially entrapped in the pores of the support and containing functionalized pendent groups to which an enzyme may be coupled to form an immobilized enzyme conjugate.
Inventors
- 👤 Ronald P. Rohrbach
- 👤 Joseph Levy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 19:48
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12N11/02; C12N11/08; C12N11/082; Y; Y10; Y10T; Y10T428/00; Y10T428/249921; Y10T428/249953; Y10T428/249954; Y10T428/249955; Y10T428/249958; Y10T428/249967; Y10T428/24997; Y10T428/249981; Y10T428/31504; Y10T428/31855; Y10T428/31938
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
90 words
An organic-inorganic matrix having pendent covalent bonding sites for enzymatic conjugates which comprises a porous, water-insoluble, solid inorganic support containing a copolymeric material formed by the in situ reaction product of a water-soluble aminopolystyrene in an aqueous solution possessing a pH of less than 7.0 and a bifunctional monomer selected from the group consisting of organic compounds containing carbonyl, acyl and isocyanato moieties, wherein said bifunctional monomer is added in a molar excess of 2 to 50 moles of monomer per mol of the amine content of said water-soluble aminopolystyrene.
Claim 2
Dependent Claim
90 words
An organic-inorganic matrix having pendent covalent bonding sites for enzymatic conjugates which comprises a porous, water-insoluble, solid inorganic support containing a copolymeric material formed by the in situ reaction product of a water-soluble aminopolystyrene in an aqueous solution possessing a pH of less than 7.0 and a bifunctional monomer selected from the group consisting of organic compounds containing carbonyl, acyl and isocyanato moieties, wherein said bifunctional monomer is added in a molar excess of 2 to 50 moles of monomer per mol of the amine content of said water-soluble aminopolystyrene.
Claim 3
Dependent Claim
17 words
The matrix as set forth in claim 1 in which said solid support comprises a metallic oxide.
Claim 4
Dependent Claim
16 words
The matrix as set forth in claim 2 in which said metallic oxide is an alumina.
Claim 5
Dependent Claim
14 words
The matrix as set forth in claim 3 in which said alumina is gamma-alumina.
Claim 6
Dependent Claim
23 words
The matrix as set forth in claim 1 in which said solid support is a ceramic monolith coated with a porous metallic oxide.
Claim 7
Dependent Claim
17 words
The matrix as set forth in claim 1 in which said solid support is a porous silica.
Claim 8
Dependent Claim
15 words
The matrix as set forth in claim 1 in which the bifunctional monomer is glutaraldehyde.
Claim 9
Dependent Claim
52 words
The organic-inorganic matrix as set forth in claim 1 which is prepared by depositing a water soluble salt of aminopolystyrene on a solid support from an aqueous solution at a pH less than 7 and thereafter reacting the resultant aminopolystyrene-solid support composite with said bifunctional monomer to form the desired organic-inorganic matrix.
Claim 10
Dependent Claim
23 words
The matrix as set forth in claim 8 in which the pH is in a range of from about 1 to about 4.
Claim 11
Dependent Claim
21 words
The matrix as set forth in claim 8 in which said water soluble salt of aminopolystyrene is the hydrochloric acid salt.
Claim 12
Dependent Claim
20 words
The matrix as set forth in claim 8 in which said water soluble salt of aminopolystyrene is sulfuric acid salt.
Claim 13
Dependent Claim
15 words
The matrix as set forth in claim 8 in which the bifunctional monomer is glutaraldehyde.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-06-03 | 1983-12-03 | 1984-06-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-06-03 | 1987-12-03 | 1988-06-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-06-03 | 1991-12-03 | 1992-06-03 | $7700.00 | Window Closed / Lapsed |