Porous inorganic support material coated with an organic stationary phase, for use in chromatography, and process for its preparation

Claim 1

A support material, usable as such in chromatography or as a starting material which, upon linkage to it of compounds containing ionic groups, ligands or biomacromolecules, can be used as ion exchanger, as a medium in affinity chromatography or in enzymatic reactions, consisting of porous, inorganic silanized particles onto which there is covalently bonded an organic, stationary phase built up of (co)polymerized vinyl monomers and containing amide groups, characterized in that (a) a hydroxyl-containing inorganic material has been silanized with an organo silane of the formula I ##STR12## wherein n=1-5; R 1 is halogen or alkoxy (1-10 C); R 2 and R 3 are each halogen, alkoxy (1-10 C), alkyl (1-3 C) or aryl (6-10 C), the halogen and alkoxy group in R 1 , R 2 and R 3 being capable of forming an --O--Si-bond between the silane and the inorganic material through its surface hydroxyl groups; and R 4 is amino, substituted amino, mercapto, an ester group or glycidoxy; and (B) the organic stationary phase is a crosslinked (co)polymer obtained by addition polymerization and built up of 0.1 to 140 μmoles of vinyl monomers per m 2 inorganic surface of the support material, said (co)polymer containing at least 0.1 μmoles amide groups per m 2 inorganic surface of the support material, and said vinyl monomers consisting wholly or partly of bifunctional vinyl monomers containing amide groups and having the formula II ##STR13## wherein R 5 and R 6 each are hydrogen or an organic group having up to 18 carbon atoms; R 7 is hydrogen or an organic group up to 20 carbon atoms; and m is an integer from 1 to 20.