Patent US5262035A successfully refreshed from Google Patents.
US5262035A
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US
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Enzyme electrodes
Assignee: Abbott Diabetes Care Inc
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Filing: 1989-08-02
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Granted: 1993-11-16
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Expires: 2009-08-02
Abstract
Abstract Enzyme electrodes having a surface coated with a film. The film is formed from materials in which a redox enzyme is covalently bonded to a three dimensional molecular structure. The molecular structure is of the class having multiple redox centers, for example, a crosslinked redox polymer.
Inventors
- 👤 Brian A. Gregg
- 👤 Adam Heller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 15:13
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; C12Q1/002; Y; Y10; Y10S; Y10S435/00; Y10S435/817; Y10S436/00; Y10S436/806
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
41 words
An electrode having a surface coated with a film, the film comprising: a crosslinked polymer having multiple redox centers; and a redox enzyme bound within the crosslinked polymer, wherein the crosslinked polymer provides electrical contact between the electrode and the enzyme.
Claim 2
Dependent Claim
41 words
An electrode having a surface coated with a film, the film comprising: a crosslinked polymer having multiple redox centers; and a redox enzyme bound within the crosslinked polymer, wherein the crosslinked polymer provides electrical contact between the electrode and the enzyme.
Claim 3
Dependent Claim
24 words
The electrode of claim 1, wherein the crosslinked polymer includes a plurality of transition metal complexes, each complex having a plurality of organic ligands.
Claim 4
Dependent Claim
11 words
The electrode of claim 2, wherein the transition metal comprises osmium.
Claim 5
Dependent Claim
16 words
The electrode of claim 1, wherein the crosslinked polymer includes a plurality of organic redox centers.
Claim 6
Dependent Claim
11 words
An amperometric biosensor having an electrode as recited in claim 1.
Claim 7
Dependent Claim
27 words
The amperometric biosensor of claim 5, wherein the electrode is capable of selectively sensing one of the following biochemicals: glucose, lactate, glycerol-3-phosphate, L-amino acids, or D-amino acids.
Claim 8
Dependent Claim
15 words
The amperometric biosensor of claim 5, wherein the electrode is capable of selectively sensing nitrate.
Claim 9
Dependent Claim
10 words
A bioelectrosynthesizer having an electrode as recited in claim 1.
Claim 10
Dependent Claim
16 words
The electrode of claim 1, wherein the redox enzyme is covalently bonded to the crosslinked polymer.
Claim 11
Dependent Claim
42 words
An electrode having a surface coated with a film, the film comprising: a hydrophilic cross-linked polymer having multiple redox centers; and a redox enzyme bound within the cross-linked polymer, wherein the cross-linked polymer provides electrical contact between the electrode and the enzyme.
Claim 12
Dependent Claim
42 words
An electrode having a surface coated with a film, the film comprising: a hydrophilic cross-linked polymer having multiple redox centers; and a redox enzyme bound within the cross-linked polymer, wherein the cross-linked polymer provides electrical contact between the electrode and the enzyme.
Claim 13
Dependent Claim
59 words
An electrode made of a material selected from the group consisting of gold, platinum, glassy carbon and graphite, said electrode having a surface coated with a film, the film comprising: a cross-linked polymer having multiple redox centers; and a redox enzyme bound within the cross-linked polymer, wherein the cross-linked polymer provides electrical contact between the electrode and the enzyme.
Claim 14
Dependent Claim
59 words
An electrode made of a material selected from the group consisting of gold, platinum, glassy carbon and graphite, said electrode having a surface coated with a film, the film comprising: a cross-linked polymer having multiple redox centers; and a redox enzyme bound within the cross-linked polymer, wherein the cross-linked polymer provides electrical contact between the electrode and the enzyme.
Claim 15
Dependent Claim
24 words
The electrode of claim 11, wherein the resulting electrode exhibits current densities in excess of 10 micro Amps/cm 2 in the presence of substrate.
Claim 16
Dependent Claim
24 words
The electrode of claim 12, wherein the resulting electrode exhibits current densities in excess of 100 micro Amps/cm 2 in the presence of substrate.
Claim 17
Dependent Claim
24 words
The electrode of claim 13, wherein the resulting electrode exhibits current densities in excess of 1000 micro Amps/cm 2 in the presence of substrate.
Claim 18
Dependent Claim
62 words
An electrode having a surface coated with a film, the film comprising: a cross-linked polymer having multiple redox centers; and a redox enzyme bound within the cross-linked polymer, wherein the cross-linked polymer provides electrical contact between the electrode and the enzyme, and wherein the resulting electrode has an operating potential in the range of 0.1V to 0.5V versus the Standard Calomel Electrode.
Claim 19
Dependent Claim
62 words
An electrode having a surface coated with a film, the film comprising: a cross-linked polymer having multiple redox centers; and a redox enzyme bound within the cross-linked polymer, wherein the cross-linked polymer provides electrical contact between the electrode and the enzyme, and wherein the resulting electrode has an operating potential in the range of 0.1V to 0.5V versus the Standard Calomel Electrode.
Claim 20
Dependent Claim
61 words
An electrode having a surface coated with a film, the film comprising: a cross-linked polymer having multiple redox centers; and a redox enzyme bound within the cross-linked polymer, wherein the cross-linked polymer provides electrical contact between the electrode and the enzyme, and wherein the resulting electrode exhibits current densities in excess of 10 micro Amps/cm 2 in the presence of substrate.
Claim 21
Dependent Claim
61 words
An electrode having a surface coated with a film, the film comprising: a cross-linked polymer having multiple redox centers; and a redox enzyme bound within the cross-linked polymer, wherein the cross-linked polymer provides electrical contact between the electrode and the enzyme, and wherein the resulting electrode exhibits current densities in excess of 10 micro Amps/cm 2 in the presence of substrate.
Claim 22
Dependent Claim
20 words
The electrode of claim 16 wherein the resulting electrode exhibits current densities in excess of 100 micro Amps/cm 2 .
Claim 23
Dependent Claim
20 words
The electrode of claim 17 wherein the resulting electrode exhibits current densities in excess of 1000 micro Amps/cm 2 .
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-11-15 | 1997-05-17 | 1997-11-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-11-15 | 2001-05-17 | 2001-11-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-11-15 | 2005-05-17 | 2005-11-16 | $7700.00 | Window Closed / Lapsed |