EP0401735A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Process for producing -L-tryptophan
Assignee: KH Neochem Co Ltd
•
Filing: 1990-06-05
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Granted: —
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Expires: 2010-06-05
Abstract
Abstract L-tryptophan is produced by constructing a recombinant DNA composed of a vector DNA and DNA fragments bearing all genetic information relating to the synthesis of DS, AS, PRT, PRAI, InGPS, TS and PGDH, introducing the recombinant DNA into a microorganism belonging to the genus Corynebacterium or Brevibacterium , culturing the microorganism in a medium, and recovering L-tryptophan accumulated in the culture medium.
Inventors
- 👤 Katsumata Ryoichi
- 👤 Ikeda Masato
- 👤 Nakanishi Keiko
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 08:50
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/22; C12P13/227; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N15/63; C12N15/74; C12N15/77
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
74 words
(1) A process for producing L-tryptophan which comprises culturing in a culture medium a microorganism belonging to the genus Corynebacterium or Brevibacterium and carrying a recombinant DNA composed of a vector DNA and DNA fragments bearing all genetic information relating to the synthesis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, anthranilate synthase, anthranilate phosphoribosyl transferase, N-5′-phosphoribosyl anthranilate isomerase, indole-3-glycerol phosphate synthase, tryptophan synthase and 3-phosphoglycerate dehydrogenase; allowing L-tryptophan to accumulate in the culture medium; and recovering L-tryptophan therefrom.
Claim 2
Dependent Claim
24 words
(2) A process according to claim 1, wherein said DNA fragments are derived from a microorganism belonging to the genus Corynebacterium or Brevibacterium .
Claim 3
Dependent Claim
76 words
(3) A recombinant DNA composed of a vector DNA and DNA fragments derived from a microorganism belonging to the genus Corynebacterium or Brevibacterium , said DNA fragments bearing all genetic information relating to the synthesis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, anthranilate synthase, anthranilate phosphoribosyl transferase, N-5′-phosphoribosyl anthranilate isomerase, indole-3-glycerol phosphate synthase, tryptophan synthase and 3-phosphoglycerate dehydrogenase, and being capable of imparting resistance to the analogues of tryptophan to a microorganism belonging to the genus Corynebacterium or Brevibacterium .
Claim 4
Dependent Claim
65 words
(4) A microorganism belonging to the genus Corynebacterium or Brevibacterium and carrying a recombinant DNA which is composed of a vector DNA and DNA fragments derived from a microorganism belonging to the genus Corynebacterium or Brevibacterium and which bears all genetic information relating to the synthesis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, anthranilate synthase, anthranilate phosphoribosyl transferase, N-5′-phosphoribosyl anthranilate isomerase, indole-3-glycerol phosphate synthase, tryptophan synthase and 3-phosphoglycerate dehydrogenase.
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