Patent US1230726A successfully refreshed from Google Patents.
US4500640A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Plasmids
Assignee: KH Neochem Co Ltd
•
Filing: 1982-04-13
•
Granted: 1985-02-19
•
Expires: 2002-04-13
Abstract
Abstract Disclosed are novel plasmids which can replicate autonomously in microorganisms belonging to the genus Corynebacterium or Brevibacterium and which carry a gene for resistance to streptomycin and/or spectinomycin. The plasmids are useful as a cloning vector.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Tetsuo Oka
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:01
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/13; C12R2001/15; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/843
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
56 words
An isolated plasmid pCG 4 characterized by a molecular weight of about 19 megadaltons, a number of cleavage sites for the restriction endonucleases, Eco RI, Bam HI, Hind III, Pst I and Sal I of 4, 7, 9, 6 and 6 respectively, and carrying a gene for resistance to a least one of streptomycin and spectinomycin.
Claim 2
Dependent Claim
56 words
An isolated plasmid pCG 4 characterized by a molecular weight of about 19 megadaltons, a number of cleavage sites for the restriction endonucleases, Eco RI, Bam HI, Hind III, Pst I and Sal I of 4, 7, 9, 6 and 6 respectively, and carrying a gene for resistance to a least one of streptomycin and spectinomycin.
Claim 3
Dependent Claim
28 words
A recombinant plasmid capable of autonomous replication in Corynebacterium or Brevibacterium constructed from the plasmid pCG 4 defined in claim 1 by adding a foreign DNA fragment thereto.
Claim 4
Dependent Claim
56 words
A recombinant plasmid capable of autonomous replication in Corynebacterium or Brevibacterium constructed from the plasmid pcG 4 defined in claim 1 by deleting a DNA region that does not affect its function to replicate autonomously or its resistance to at least one of said streptomycin and spectinomycin and adding a foreign DNA fragment to said plasmid.
Claim 5
Dependent Claim
47 words
A recombinant plasmid constructed from plasmid pCG 4 defined in claim 1 by deleting a DNA region that does not affect its resistance to at least one of streptomycin and spectinomycin and adding a foreign DNA region conferring a function to replicate autonomously in Corynebacterium or Brevibacterium.
Claim 6
Dependent Claim
28 words
A recombinant plasmid according to claim 4 which is plasmid pCG 11 characterized by the restriction map illustrated in FIG. 1 and a molecular weight of 4.5 megadaltons.
Claim 7
Dependent Claim
19 words
A recombinant plasmid constructed from plasmid pCG 11 defined in claim 5 by adding a foreign DNA fragment thereto.
Claim 8
Dependent Claim
20 words
A biologically pure culture of the microorganism Corynebacterium glutamicum LA 103/pCG 11, ATCC 39022 which carries the plasmid pCG 11.
Claim 9
Dependent Claim
20 words
A biologically pure culture of the microorganism Corynebacterium glutamicum LA 103/pCG 11, ATCC 39022 which carries the plasmid pCG 11.
Claim 10
Dependent Claim
24 words
A biologically pure culture of the microorganism Corynebacterium glutamicum 225-250, having the identifying characteristics of ATCC 31830 and which carries the plasmid pCG 4.
Claim 11
Dependent Claim
24 words
A biologically pure culture of the microorganism Corynebacterium glutamicum 225-250, having the identifying characteristics of ATCC 31830 and which carries the plasmid pCG 4.
Claim 12
Dependent Claim
45 words
A biologically pure culture of a microorganism selected from the group consisting of Corynebacterium herculis ATCC 13868/pCG 4, Brevibacterium flavum ATCC 14067/pCG 4 and Brevibacterium lactofermentum ATCC13655/pCG 4 having the identifying characteristics of ATCC 31837, 31838 and 31839 respectively and carrying the plasmid pCG 4.
Claim 13
Dependent Claim
45 words
A biologically pure culture of a microorganism selected from the group consisting of Corynebacterium herculis ATCC 13868/pCG 4, Brevibacterium flavum ATCC 14067/pCG 4 and Brevibacterium lactofermentum ATCC13655/pCG 4 having the identifying characteristics of ATCC 31837, 31838 and 31839 respectively and carrying the plasmid pCG 4.
References Cited (Backward Citations)
US3003925A
Cited
US3096252A
Cited
US3002889A
Cited
US3120472A
Cited
US3128237A
Cited
US3220929A
Cited
GB2076853A
Cited
FR2482622A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-02-19 | 1988-08-20 | 1989-02-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-02-19 | 1992-08-20 | 1993-02-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-02-19 | 1996-08-20 | 1997-02-19 | $7700.00 | Window Closed / Lapsed |