Patent US4500640A successfully refreshed from Google Patents.
US4500640A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Plasmids
Assignee: KH Neochem Co Ltd
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Filing: 1982-04-13
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Granted: 1985-02-19
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Expires: 2002-04-13
Abstract
Abstract Disclosed are novel plasmids which can replicate autonomously in microorganisms belonging to the genus Corynebacterium or Brevibacterium and which carry a gene for resistance to streptomycin and/or spectinomycin. The plasmids are useful as a cloning vector.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Tetsuo Oka
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:18
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/13; C12R2001/15; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/843
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
56 words
An isolated plasmid pCG 4 characterized by a molecular weight of about 19 megadaltons, a number of cleavage sites for the restriction endonucleases, Eco RI, Bam HI, Hind III, Pst I and Sal I of 4, 7, 9, 6 and 6 respectively, and carrying a gene for resistance to a least one of streptomycin and spectinomycin.
Claim 2
Dependent Claim
56 words
An isolated plasmid pCG 4 characterized by a molecular weight of about 19 megadaltons, a number of cleavage sites for the restriction endonucleases, Eco RI, Bam HI, Hind III, Pst I and Sal I of 4, 7, 9, 6 and 6 respectively, and carrying a gene for resistance to a least one of streptomycin and spectinomycin.
Claim 3
Dependent Claim
28 words
A recombinant plasmid capable of autonomous replication in Corynebacterium or Brevibacterium constructed from the plasmid pCG 4 defined in claim 1 by adding a foreign DNA fragment thereto.
Claim 4
Dependent Claim
56 words
A recombinant plasmid capable of autonomous replication in Corynebacterium or Brevibacterium constructed from the plasmid pcG 4 defined in claim 1 by deleting a DNA region that does not affect its function to replicate autonomously or its resistance to at least one of said streptomycin and spectinomycin and adding a foreign DNA fragment to said plasmid.
Claim 5
Dependent Claim
47 words
A recombinant plasmid constructed from plasmid pCG 4 defined in claim 1 by deleting a DNA region that does not affect its resistance to at least one of streptomycin and spectinomycin and adding a foreign DNA region conferring a function to replicate autonomously in Corynebacterium or Brevibacterium.
Claim 6
Dependent Claim
28 words
A recombinant plasmid according to claim 4 which is plasmid pCG 11 characterized by the restriction map illustrated in FIG. 1 and a molecular weight of 4.5 megadaltons.
Claim 7
Dependent Claim
19 words
A recombinant plasmid constructed from plasmid pCG 11 defined in claim 5 by adding a foreign DNA fragment thereto.
Claim 8
Dependent Claim
20 words
A biologically pure culture of the microorganism Corynebacterium glutamicum LA 103/pCG 11, ATCC 39022 which carries the plasmid pCG 11.
Claim 9
Dependent Claim
20 words
A biologically pure culture of the microorganism Corynebacterium glutamicum LA 103/pCG 11, ATCC 39022 which carries the plasmid pCG 11.
Claim 10
Dependent Claim
24 words
A biologically pure culture of the microorganism Corynebacterium glutamicum 225-250, having the identifying characteristics of ATCC 31830 and which carries the plasmid pCG 4.
Claim 11
Dependent Claim
24 words
A biologically pure culture of the microorganism Corynebacterium glutamicum 225-250, having the identifying characteristics of ATCC 31830 and which carries the plasmid pCG 4.
Claim 12
Dependent Claim
45 words
A biologically pure culture of a microorganism selected from the group consisting of Corynebacterium herculis ATCC 13868/pCG 4, Brevibacterium flavum ATCC 14067/pCG 4 and Brevibacterium lactofermentum ATCC13655/pCG 4 having the identifying characteristics of ATCC 31837, 31838 and 31839 respectively and carrying the plasmid pCG 4.
Claim 13
Dependent Claim
45 words
A biologically pure culture of a microorganism selected from the group consisting of Corynebacterium herculis ATCC 13868/pCG 4, Brevibacterium flavum ATCC 14067/pCG 4 and Brevibacterium lactofermentum ATCC13655/pCG 4 having the identifying characteristics of ATCC 31837, 31838 and 31839 respectively and carrying the plasmid pCG 4.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-02-19 | 1988-08-20 | 1989-02-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-02-19 | 1992-08-20 | 1993-02-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-02-19 | 1996-08-20 | 1997-02-19 | $7700.00 | Window Closed / Lapsed |