EP0064680B1
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EP
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Novel lysozyme-sensitive microorganism
Assignee: KH Neochem Co Ltd
•
Filing: 1982-04-29
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Granted: 1987-09-16
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Expires: 2002-04-29
Abstract
No abstract record available.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Tetsuo Oka
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 09:02
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77; C12R; C12R2001/00; C12R2001/01; C12R2001/13; C12R2001/15; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/843
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
24 words
A lysozyme-sensitive microorganism belonging to the genus Corynebacterium or Brevibacterium which fails to grow on a nutrient medium containing 1.6 to 3.2 Ilg/ml lysozyme.
Claim 2
Dependent Claim
24 words
A lysozyme-sensitive microorganism belonging to the genus Corynebacterium or Brevibacterium which fails to grow on a nutrient medium containing 1.6 to 3.2 Ilg/ml lysozyme.
Claim 3
Dependent Claim
19 words
A microorganism according to Claim 1, which is selected from Corynebacterium glutamicum, Corynebacterium herculis, Brevibacterium divaricatum and Brevibacterium lactofermentum.
Claim 4
Dependent Claim
39 words
A microorganism according to Claim 2, which is selected from Corynebacterium glutamicum L-15 (FERM P-5946, ATCC 31834), Corynebacterium herculis L-103 (FERM P-5947, ATCC 31866), Brevibacterium divaricatum L-204 (FERM P-5948, ATCC 31867) and Brevibacterium lactofermentum L-312 (FERM P-5949, ATCC 31868).
Claim 5
Dependent Claim
32 words
A process for preparing a protoplast of a microorganism belonging to the genus Corynebacterium or Brevibacterium which comprises treating a microorganism according to any one of Claims 1 to 3 with lysozyme.
Claim 6
Dependent Claim
53 words
A process for transforming a microorganism belonging to the genus Corynebacterium or Brevibacterium which comprises preparing a protoplast of a microorganism belonging to the genus Corynebacterium or Brevibacterium by treating a microorganism according to any one of Claims 1 to 3 with lysozyme, introducing deoxyribonucleic acid into the protoplast and regenerating the protoplast.
Claim 7
Dependent Claim
31 words
A lysozyme-sensitive microorganism belonging to the genus Corynebacterium or Brevibacterium according to any one of Claims 1 to 3 which carries the plasmid pCG4 derived from Corynebacterium glutamicum 225-250 (FERM P-5939).
References Cited (Backward Citations)
No citation records indexed.
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