EP0124048A2
Expired / Lapsed
EP
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Method for producing L-tryptophan by fermentation
Assignee: Ajinomoto Co Inc
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Filing: 1984-04-19
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Granted: —
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Expires: 2004-04-19
Abstract
Abstract A microorganism strain capable of producing L-tryptophan, is constructed by inserting a chromosomal DNA fragment with the genetic information for the L-tryptophan biosynthesis, which has been derived from glutamic acid producing bacteria, into a vector plasmid capable of self-replicating in cells of Coryneform glutamic acid-producing bacteria, and incorporating the recombinant plasmid into a DNA-recipient belonging to Coryneform glutamicum acid-producing bacteria and isolating the transformed strain. …<??>By cultivating the obtained strain in a suitable medium L-tryptophan is produced in high yields.
Inventors
- 👤 Kiyoshi Miwa
- 👤 Shigeru Nakamori
- 👤 Konosuke Sano
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 09:01
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77; C12N15/11; C12N15/52; C12P; C12P13/00; C12P13/04; C12P13/22; C12P13/227; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/843
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
76 words
A method for constructing a microorganism strain capable of producing L-tryptophan,by genetic transformation, characterized by the following steps : (a) Inserting a chromosomal DNA fragment with the genetic information for the L-tryptophan biosynthesis, which has been derived from glutamic acid producing bacteria, into a vector plasmid capable of self-replicating in cells of Coryneform glutamic acid-producing bacteria, and (b) incorporating the recombinant plasmid into a DNA-recipient belonging to Coryneform glutamicum acid-producing bacteria and isolating the transformed strain.
Claim 2
Independent Claim
76 words
A method for constructing a microorganism strain capable of producing L-tryptophan,by genetic transformation, characterized by the following steps : (a) Inserting a chromosomal DNA fragment with the genetic information for the L-tryptophan biosynthesis, which has been derived from glutamic acid producing bacteria, into a vector plasmid capable of self-replicating in cells of Coryneform glutamic acid-producing bacteria, and (b) incorporating the recombinant plasmid into a DNA-recipient belonging to Coryneform glutamicum acid-producing bacteria and isolating the transformed strain.
Claim 3
Dependent Claim
27 words
The method of claim 1, in which the chromosomal DNA fragment is derived from a DNA donor belonging to Coryneform glutamic acid-producing bacteria or to Escherichia coli.
Claim 4
Dependent Claim
33 words
The method of claim 1 or 2, in which the recombinant plasmid is incorporated into a recipient which is a mutant of Coryneform glutamic acid-producing bacteria requiring at least L-tryptophan for its growth.
Claim 5
Independent Claim
26 words
The method of any of the claims 1 to 3, wherein the chromosomal DNA fragment is obtained from glutamic acid-producing bacteria resistant to a tryptophan antagonist.
Claim 6
Independent Claim
30 words
The method of any of the claims 1 to 3, wherein the donor of the vector DNA is a strain of Coryneform glutamic acid-producing bacteria resistant to a tryptophan antagonist.
Claim 7
Independent Claim
24 words
The method of any of the claims 1 to 5, wherein the DNA-recipient belongs to Coryneform glutamic acid-producing bacteria resistant to a tryptophan antagonist.
Claim 8
Dependent Claim
24 words
Microorganism strains, capable of producing L-tryptophan, obtainable according to the method of any of the claims 1 to 6,and L-tryptophan producing artificial mutants thereof.
Claim 9
Dependent Claim
27 words
Recombinant plasmid including the genetic information for the biosynthesis of L-tryptophan or its precursors, obtainable according to the method of any of the claims 1 to 6.
Claim 10
Independent Claim
41 words
A method for producing L-tryptophan by culturing a microorganism capable of producing L-tryptophan in a culture medium and recovering the L-tryptophan accumulated in the culture medium, characterized by using a microorganism obtained according to any of the claims 1 to 6.
Claim 11
Dependent Claim
40 words
A method for producing L-tryptophan by culturing a microorganism capable of producing L-tryptophan in a culture medium and recovering the L-tryptophan accumulated in the culture medium, characterized by the use of a microorganism incorporating the recombinant plasmid of claim 7.
References Cited (Backward Citations)
No citation records indexed.
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