EP0136359A1
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EP
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Process for preparing l-histidine
Assignee: KH Neochem Co Ltd
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Filing: 1984-02-16
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Granted: —
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Expires: 2004-02-16
Abstract
Abstract A process for preparing amino acid, which comprises culturing transformed strain obtained by transforming host strain selected from microorganisms to Corynebacterium or Brevibacterium using recombinant DNA between a DNA fragment containing genes relating to an amino acid biosynthesis and vector DNA to accumulate the amino acid in the culture liquor, and recovering the amino acid.
Inventors
- 👤 Tetsuo Oka
- 👤 Ryoichi Katsumata
- 👤 Akio Ozaki
- 👤 Toru Mizukami
- 👤 Haruhiko Yokoi
- 👤 Masako Hara
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 09:06
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N15/63; C12N15/74; C12N15/77; C12N9/00; C12N9/10; C12N9/12; C12P13/06; C12P13/10; C12P13/22; C12P13/222; C12P13/24
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
68 words
A process for producing an amino acid which comprises transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene encoding for the enzyme involved in the biosynthesis of the amino acid and a vector DNA, culturing the transformant in a nutrient medium, accumulating the amino acid in the culture medium and recovering the amino acid therefrom.
Claim 2
Dependent Claim
68 words
A process for producing an amino acid which comprises transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene encoding for the enzyme involved in the biosynthesis of the amino acid and a vector DNA, culturing the transformant in a nutrient medium, accumulating the amino acid in the culture medium and recovering the amino acid therefrom.
Claim 3
Dependent Claim
18 words
The process according to claim 1, wherein the amino acid is histidine, tryptophan, phenylalanine, isoleucine, tyrosine or arginine.
Claim 4
Dependent Claim
22 words
The process according to claim 1, wherein the DNA fragment containing said gene is derived from prokaryotes, eukaryotes, bacteriophages, viruses or plasmids.
Claim 5
Dependent Claim
26 words
The process according to claim 3, wherein the prokaryote is selected from bacteria belonging to the genus Escherichia, Corynebacterium, Brevibacterium, Microbacterium, Bacillus, Staphylococcus, Streptococcus or Serratia.
Claim 6
Dependent Claim
20 words
The process according to claim 4, wherein the bacterium is resistant to histidine analog, tryptophan analog, phenylalanine or phenylalanine analog.
Claim 7
Dependent Claim
19 words
The process according to claim 5, wherein the .bacterium is resistant to l,2,4-triazole-3-alanine, 4-methyltryptophan, 5-methyltryptophan and 6-methyltryptophan or para-fluorophenylalanine.
Claim 8
Dependent Claim
16 words
The process according to claim 1, wherein the DNA fragment contains an anthranylic acid synthetase gene.
Claim 9
Dependent Claim
19 words
The process according to claim 7, wherein the DNA fragment can confer resistance to tryptophan analog on a microorganism.
Claim 10
Dependent Claim
17 words
The process according to claim 8, wherein the tryptophan analog is selected from 4-methyltryptophan, 5-methyltryptophan and 6-methyltryptophan.
Claim 11
Dependent Claim
21 words
The process according to claim 7, wherein the inhibition of the anthranylic acid synthetase with tryptophan or tryptophan analogs are released.
Claim 12
Dependent Claim
24 words
The process according to claim 1, wherein the DNA fragment contains a gene of 3-deoxy-2-keto-D-arabino-heptulosonate 7-phosphate synthetase, chorismate mutase, prephenate dehydrogenase or pretyrosine aminotransferase.
Claim 13
Dependent Claim
22 words
The process according to claim 11, wherein the DNA fragment confers resistance to phenylalanine, tyrosine or an analog thereof on a microorganism.
Claim 14
Dependent Claim
28 words
The process according to claim 1, wherein the DNA fragment contains a gene coding for the enzyme involved in the biosynthesis of threonine from aspartic acid to threonine.
Claim 15
Dependent Claim
22 words
The process according to claim 1, wherein the DNA fragment can confer resistance to histidine or a histidine analog on a microorganism.
Claim 16
Dependent Claim
27 words
The process according to claim 1, wherein the vector is a vector derived from a microorganism belonging to the genus Corynebacterium or Brevibacterium or a derivative thereof.
Claim 17
Dependent Claim
22 words
The process according to claim 15, wherein the vector is selected from pCGl, pCG2, pCG4, pCGll, pCE51, pCE52, pCE53, pCE54 and pCBl01.
Claim 18
Dependent Claim
16 words
The process according to claim 1, wherein the microorganism belongs to the genus Corvnebacterium or Brevibacterium.
Claim 19
Dependent Claim
13 words
The process according to claim 17, wherein the microorganism has an amino acid-productivity.
Claim 20
Dependent Claim
23 words
The process according to claim 17, wherein the microorganism is selected from the species Corynebacterium qlutamicum, Corynebacterium herculis, Brevibacterium flavum and Brevibacterium lactofermentum.
Claim 21
Dependent Claim
45 words
A microorganism belonging to the genus Corynebacterium or Brevibacterium and containing a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of an amino acid derived from a microorganism belonging to the genus Escherichia, Corynebacterium or Brevibacterium and a vector DNA.
Claim 22
Dependent Claim
45 words
A microorganism belonging to the genus Corynebacterium or Brevibacterium and containing a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of an amino acid derived from a microorganism belonging to the genus Escherichia, Corynebacterium or Brevibacterium and a vector DNA.
Claim 23
Dependent Claim
12 words
The microorganism according to claim 20, wherein the amino acid is histidine.
Claim 24
Dependent Claim
34 words
The microorganism according to claim 21, which is Corynebacterium glutamicum K32, ATCC 39281, Corynebacterium glutamicum K49, FERM BP-464, Corynebacterium herculis K33, ATCC 39282, Brevibacterium flavum K34, ATCC 39283 or Brevibacterium lactofermentum K35, ACTT 39284.
Claim 25
Dependent Claim
12 words
The microorganism according to claim 20, wherein the amino acid is tryptophan.
Claim 26
Dependent Claim
23 words
The microorganism according to claim 23, which is Corynebacterium glutamicum K20, ATCC.39035, Corynebacterium glutamicum K31, ATCC 39280 or Corynebacterium glutamicum K37, ATCC 39285.
Claim 27
Dependent Claim
12 words
The microorganism according to claim 20, wherein the amino acid is phenylalanine.
Claim 28
Dependent Claim
13 words
The microorganism according to claim 25, which is Corynebacterium glutamicum K39, FERM P-7088.
Claim 29
Dependent Claim
12 words
The microorganism according to claim 20, wherein the amino acid is isoleucine.
Claim 30
Dependent Claim
19 words
The microorganism according to claim 27, which is Corynebacterium glutamicum K41, FERM P-7161 or Brevibacterium flavum K42, FERM BP-355.
Claim 31
Dependent Claim
12 words
The microorganism according to claim 20, wherein the amino acid is tyrosine.
Claim 32
Dependent Claim
19 words
The microorganism according to claim 29, which is Corynebacterium glutamicum K44, FERM P-7163 or Corynebacterium glutamicum K45, FERM P-7164.
Claim 33
Dependent Claim
12 words
The microorganism according to claim 20, wherein the amino acid is arginine.
Claim 34
Dependent Claim
19 words
The microorganism according to claim 31, which is Corynebacterium glutamicum K46, FERM BP-356 or Brevibacterium flavum K48, FERM BP-357.
References Cited (Backward Citations)
No citation records indexed.
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