EP0063764A1
Expired / Lapsed
EP
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Transformation method of a microorganism
Assignee: KH Neochem Co Ltd
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Filing: 1982-04-16
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Granted: —
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Expires: 2002-04-16
Abstract
Abstract Disclosed is a method for transformation of microorganisms belonging to the genera Corynebacterium and Brevibacterium whereby a foreign DNA may be introduced into a host cell and autonomously replicated.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Akio Ozaki
- 👤 Tetsuo Oka
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 09:04
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/13; C12R2001/15
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
78 words
A method for transformation of microorganisms belonging to the genera Corynebacterium and Brevibacterium which comprises incubating protoplasts of a microbial strain belonging to one of said genera and a donor deoxyribonucleic acid in the presence polyethyleneglycol or polyvinylalcohol and at least one divalent metal cation to introduce said: deoxyribonucleic acid into said protoplasts, regenerating said protoplasts by culturing in a hypertonic nutrient medium and thereafter recovering a transformed strain having a phenotype derived from said donor deoxyribonucleic acid.
Claim 2
Independent Claim
78 words
A method for transformation of microorganisms belonging to the genera Corynebacterium and Brevibacterium which comprises incubating protoplasts of a microbial strain belonging to one of said genera and a donor deoxyribonucleic acid in the presence polyethyleneglycol or polyvinylalcohol and at least one divalent metal cation to introduce said: deoxyribonucleic acid into said protoplasts, regenerating said protoplasts by culturing in a hypertonic nutrient medium and thereafter recovering a transformed strain having a phenotype derived from said donor deoxyribonucleic acid.
Claim 3
Dependent Claim
23 words
A method according to claim 1 wherein said divalent metal cation is selected from the group consisting of calcium,magnesium, manganese, barium and strontium.
Claim 4
Dependent Claim
23 words
A method according to claim 2 wherein said divalent metal cation is present at a concentration of from about 1 to 100 mM.
Claim 5
Dependent Claim
19 words
A method according to claim 1 wherein said polyethyleneglycol is present at a concentration of from 5 to 60%.
Claim 6
Dependent Claim
19 words
A method according to claim 1 wherein said polyvinylalcohol is present at a concentration of from 1 to 20%.
Claim 7
Dependent Claim
17 words
A method according to claim 1 wherein said donor deoxyribonucleic acid is a plasmid or a phage.
Claim 8
Dependent Claim
21 words
A method according to claim 6 wherein said plasmid is derived from a microorganism belonging to the genus Corynebacterium or Brevibacterium.
Claim 9
Dependent Claim
12 words
A method according to claim 7 wherein said plasmid is plasmid pCG4.
References Cited (Backward Citations)
GB2076853A
Cited
FR2482622A1
Cited
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