US4617267A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Plasmid pCG1 from Corynebacterium glutamicum
Assignee: KH Neochem Co Ltd
•
Filing: 1984-01-25
•
Granted: 1986-10-14
•
Expires: 2004-01-25
Abstract
Abstract Disclosed is a novel vector, plasmid pCG1, and a process of producing plasmid pCG1 from Corynebacterium glutamicum.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:06
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
44 words
An isolated plasmid pCG1 characterized by a molecular weight of about 2 megadaltons and the following cleavage sites for restriction endonucleases: ______________________________________
Restriction Number of
enzyme cleavage sites
______________________________________
EcoRI 1
HindIII 2
HincII 2
BamHI 0
PstI 0
SalI 0
KpnI 0
______________________________________
Claim 2
Dependent Claim
44 words
An isolated plasmid pCG1 characterized by a molecular weight of about 2 megadaltons and the following cleavage sites for restriction endonucleases: ______________________________________
Restriction Number of
enzyme cleavage sites
______________________________________
EcoRI 1
HindIII 2
HincII 2
BamHI 0
PstI 0
SalI 0
KpnI 0
______________________________________
Claim 3
Dependent Claim
35 words
A process for producing plasmid pCG1 which comprises culturing Corynebacterium glutamicum 225-57 having the identifying characteristics of ATCC 31808 in a nutrient medium, disrupting the cultured cells and recovering plasmid pCG1 from the disrupted cells.
Claim 4
Dependent Claim
35 words
A process for producing plasmid pCG1 which comprises culturing Corynebacterium glutamicum 225-57 having the identifying characteristics of ATCC 31808 in a nutrient medium, disrupting the cultured cells and recovering plasmid pCG1 from the disrupted cells.
Claim 5
Dependent Claim
20 words
A biologically pure culture of Corynebacterium glutamicum having the identifying characteristics of ATCC 31808 from which plasmid pCG1 is recoverable.
Claim 6
Dependent Claim
20 words
A biologically pure culture of Corynebacterium glutamicum having the identifying characteristics of ATCC 31808 from which plasmid pCG1 is recoverable.
Claim 7
Dependent Claim
25 words
A recombinant plasmid capable of autonomous replication in Corynebacterium constructed from the plasmid pCG1 defined in claim 1 by adding a foreign DNA fragment thereto.
Claim 8
Dependent Claim
41 words
A recombinant plasmid capable of autonomous replication in Corynebacterium constructed from the plasmid pCG1 defined in claim 1 by deleting a DNA region that does not affect its function to replicate autonomously and adding a foreign DNA fragment to said plasmid.
Claim 9
Dependent Claim
26 words
A biologically pure culture of a microorganism belonging to Corynebacterium glutamicum and harboring plasmid pCG1 or a recombinant plasmid as defined in claim 4 or 5.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-10-13 | 1990-04-14 | 1990-10-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-10-13 | 1994-04-14 | 1994-10-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-10-13 | 1998-04-14 | 1998-10-14 | $7700.00 | Window Closed / Lapsed |