Patent US3095246A successfully refreshed from Google Patents.
Patent US5697901A successfully refreshed from Google Patents.
US5697901A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Gene delivery by microneedle injection
Assignee: Applied Tissue Technologies LLC
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Filing: 1995-05-19
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Granted: 1997-12-16
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Expires: 2015-05-19
Abstract
Abstract Direct gene transfer of genetic material into an external or internal target cell site ("microseeding"), in optional combination with a wound treatment chamber, are particularly effective as a way of obtaining long term expression of native or non-native polypeptides in a host. A wide variety of proteins and materials can be expressed, either for secretion into the general blood and lymphatic system, or to alter the properties of the protein, for example, to not express proteins eliciting an immune response. The use of the optional wound chamber system for gene transfer to skin target sites also allows non-invasive assessment of the success of transfer by assaying for the presence of the expressed protein in wound fluid, in contrast to the prior art use of invasive techniques, such as biopsies, in order to achieve the same assessment of early expression.
Inventors
- 👤 Elof Eriksson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 09:40
CPC Classifications
A; A61; A61M; A61M37/00; A61M37/0015; A61K; A61K48/00; A61K9/00; A61K9/0012; A61K9/0019; A61K9/0021; A61K9/14; A61K9/16; A61K9/167; A61K9/1676; A61L; A61L27/00; A61L27/36; A61L27/50; A61L27/60; A61M5/00; A61M5/178; A61M5/31; A61M5/32; A61M5/3287; A61K35/00; A61K35/12; A61L2400/00; A61L2400/06; A61M2037/0023; A61M2037/003; A61M2037/0061; A61M2205/00; A61M2205/02; A61M2205/0244; C; C12; C12N; C12N2510/00
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
59 words
A method for introducing genetic material into a cell the method comprising the step of repeatedly injecting the genetic material into a plurality of cells of a target tissue at an in situ site on a human or non-human animal with a microneedle, whereby a change in the animal attributable to the delivery of the genetic material is detectable.
Claim 2
Independent Claim
59 words
A method for introducing genetic material into a cell the method comprising the step of repeatedly injecting the genetic material into a plurality of cells of a target tissue at an in situ site on a human or non-human animal with a microneedle, whereby a change in the animal attributable to the delivery of the genetic material is detectable.
Claim 3
Dependent Claim
76 words
The method of claim 1 wherein the cell is selected from the group consisting of a superficial keratinocyte, a stem cell keratinocyte, a dermal fibroblast, a smooth muscle, a striated muscle, a connective tissue, an epithelial tissue, a wall of an abdominal passage, a liver, a kidney, a stomach, an appendix, an intestine, a pancreas, a lung, a heart, a bladder, a gall bladder, a brain, an endocrine gland, a reproductive gland, and a lymphatic gland.
Claim 4
Dependent Claim
13 words
The method of claim 1 wherein the genetic material is attached to microparticles.
Claim 5
Dependent Claim
22 words
The method of claim 3 wherein the microparticles are iron oxide particles ranging in size from 0.5 to 1 micron in diameter.
Claim 6
Independent Claim
110 words
A method for delivering genetic material into a human or non-human subject, the method comprising the steps of: contacting a tip of a microneedle with a solution comprising the genetic material; penetrating the tip repeatedly into a site on a surface of a target tissue at an in situ site on the subject, to a depth in the tissue selected to allow contact between the tip and a plurality of target cells that can take up the genetic material, until a sufficient amount of the genetic material is delivered to the target cells such that a change in the subject attributable to the delivery of the genetic material is detectable.
Claim 7
Independent Claim
110 words
A method for delivering genetic material into a human or non-human subject, the method comprising the steps of: contacting a tip of a microneedle with a solution comprising the genetic material; penetrating the tip repeatedly into a site on a surface of a target tissue at an in situ site on the subject, to a depth in the tissue selected to allow contact between the tip and a plurality of target cells that can take up the genetic material, until a sufficient amount of the genetic material is delivered to the target cells such that a change in the subject attributable to the delivery of the genetic material is detectable.
Claim 8
Dependent Claim
23 words
A method as claimed in claim 5 wherein the target tissue is selected from the group consisting of skin cells and a wound.
Claim 9
Dependent Claim
16 words
A method as claimed in claim 5 wherein the target tissue is internal to the subject.
Claim 10
Dependent Claim
69 words
A method as claimed in claim 7 wherein the target tissue is selected from the group consisting of a smooth muscle, a striated muscle, a connective tissue, an epithelial tissue, a wall of an abdominal passage, a liver, a kidney, a stomach, an appendix, an intestine, a pancreas, a lung, a heart, a bladder, a gall bladder, a brain, an endocrine gland, a reproductive gland, and a lymphatic gland.
Claim 11
Dependent Claim
23 words
A method as claimed in claim 5 wherein less than about 500 micrograms of the genetic construct are delivered to the target cells.
Claim 12
Dependent Claim
24 words
A method as claimed in claim 5 wherein between about 20 and 200 micrograms of the genetic construct are delivered to the target cells.
Claim 13
Dependent Claim
29 words
A method as claimed in claim 5 wherein the penetrating step is repeated between about 500 and 5000 times per cm 2 of the surface of the target tissue.
Claim 14
Dependent Claim
28 words
A method as claimed in claim 5 wherein the penetrating step is repeated between 3000 and 4000 times per cm 2 of the surface of the target tissue.
Claim 15
Dependent Claim
22 words
A method as claimed in claim 5 wherein the tip penetrates the target tissue to a depth of less than 5 mm.
Claim 16
Dependent Claim
22 words
A method as claimed in claim 5 wherein the tip penetrates the target tissue to a depth of less than 3 mm.
Claim 17
Dependent Claim
16 words
A method as claimed in claim 5 wherein the genetic material encodes a non-native growth factor.
Claim 18
Dependent Claim
16 words
A method as claimed in claim 15 wherein the non-native growth factor is epidermal growth factor.
Claim 19
Dependent Claim
12 words
A method as claimed in claim 5 wherein the subject is non-human.
Claim 20
Dependent Claim
28 words
A method as claimed in claim 17 wherein the subject is selected from a group consisting of a dog, a cat, cattle, swine, a goat, and a sheep.
Claim 21
Dependent Claim
13 words
A method as claimed in claim 5 wherein the subject is a human.
Claim 22
Dependent Claim
26 words
A method as claimed in claim 5 further comprising the step of: placing the microneedle in a concentrated solution of hydrochloric acid before the penetrating step.
Claim 23
Independent Claim
131 words
A method for delivering genetic material into a target tissue of a subject, the method comprising the steps of: contacting a tip of a microneedle with a solution comprising the genetic material; penetrating the tip repeatedly into a site on a surface of the target tissue, to a depth selected to allow contact between the tip and a plurality of target cells that can take up the genetic material, until a sufficient amount of the genetic material is delivered to the target cells such that a change in the subject attributable to the delivery of the genetic material is detectable, wherein the target tissue is selected from the group consisting of skin cells and a wound, the method further comprising the step of covering the target cells with a protective chamber.
Claim 24
Independent Claim
131 words
A method for delivering genetic material into a target tissue of a subject, the method comprising the steps of: contacting a tip of a microneedle with a solution comprising the genetic material; penetrating the tip repeatedly into a site on a surface of the target tissue, to a depth selected to allow contact between the tip and a plurality of target cells that can take up the genetic material, until a sufficient amount of the genetic material is delivered to the target cells such that a change in the subject attributable to the delivery of the genetic material is detectable, wherein the target tissue is selected from the group consisting of skin cells and a wound, the method further comprising the step of covering the target cells with a protective chamber.
References Cited (Backward Citations)
GB641061A
Cited
US3026874A
Cited
US3288140A
Cited
US3580254A
Cited
US3814097A
Cited
US4304866A
Cited
US5036006A
Cited
US4868116A
Cited
US4980286A
Cited
US4767746A
Cited
US4767746B1
Cited
US4784737A
Cited
US4847193A
Cited
US4788971A
Cited
WO1990011092A1
Cited
US5225750A
Cited
US5262128A
Cited
US5152757A
Cited
US5451513A
Cited
WO1992015676A1
Cited
US5516670A
Cited
US5364374A
Cited
US5523222A
Cited
US5457041A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-12-15 | 2001-06-16 | 2001-12-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-12-15 | 2005-06-16 | 2005-12-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-12-15 | 2009-06-16 | 2009-12-16 | $7700.00 | Window Closed / Lapsed |