US4868116A
Expired / Lapsed
US
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Introduction and expression of foreign genetic material in epithelial cells
Assignee: Whitehead Institute for Biomedical Research
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Filing: 1986-07-09
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Granted: 1989-09-19
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Expires: 2006-07-09
Abstract
Abstract Epithelial cells expressing foreign genetic material are described. The foreign genetic material can be DNA or RNA which does not occur in epithelial cells; DNA or RNA which occurs in epithelial cells but is not expressed in them at levels which are biologically significant; DNA or RNA which occurs in epithelial and has been modified so that it is expressed in epithelial cells; and any DNA or RNA which can be modified to be expressed in epithelial cells, alone or in any combination thereof. In addition, epithelial cells of the present invention can express genetic material encoding a selectable marker by which cells expressing the foreign genetic material can be expressed.
Inventors
- 👤 Jeffrey R. Morgan
- 👤 Richard C. Mulligan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:38
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; A; A61; A61K; A61K35/00; A61K35/12; A61K35/36; A61L; A61L27/00; A61L27/50; A61L27/54; A61L27/60; C07K14/635; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C12N15/87; C12N15/90; A61K38/00; A61L2300/00; A61L2300/20; A61L2300/252; A61L2300/254; A61L2300/258; A61L2300/40; A61L2300/43; A61L2300/60; A61L2300/64; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/13011; C12N2740/13041; C12N2740/13043; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
82 words
A method of making transplantable keratinocytes which express incorporated genetic material and genetic material encoding at least one dominant selectable marker comprising: a. forming a culture of keratinocytes and fibroblast cells treated to prevent their multiplication, the fibroblast cells producing an infectious recombinant retrovirus having a recombinant genome comprised of incorporated genetic material or a culture of keratinocytes and cell products produced by said fibroblast cells; and b. maintaining said culture under conditions conducive to cell growth whereby keratinocyte colonies are formed.
Claim 2
Independent Claim
82 words
A method of making transplantable keratinocytes which express incorporated genetic material and genetic material encoding at least one dominant selectable marker comprising: a. forming a culture of keratinocytes and fibroblast cells treated to prevent their multiplication, the fibroblast cells producing an infectious recombinant retrovirus having a recombinant genome comprised of incorporated genetic material or a culture of keratinocytes and cell products produced by said fibroblast cells; and b. maintaining said culture under conditions conducive to cell growth whereby keratinocyte colonies are formed.
Claim 3
Independent Claim
99 words
A method of making transplantable sheets of keratinous tissue comprised of keratinocytes expressing incorporated genetic material, the method comprising: a. culturing kertinocytes and fibroblast cells treated to prevent their multiplication in a culture vessel, the fibroblast cells producing an infectious recombinant virus having a recombinant genome comprised of incorporated genetic material or keratinocytes and cell products produced by said fibroblast cells; b. maintaining said culture under conditions conducive to cell growth, whereby a sheet of keratinous tissue is formed on a surface of the vessel; and c. removing said sheet of keratinous tissue from the surface of the vessel.
Claim 4
Independent Claim
99 words
A method of making transplantable sheets of keratinous tissue comprised of keratinocytes expressing incorporated genetic material, the method comprising: a. culturing kertinocytes and fibroblast cells treated to prevent their multiplication in a culture vessel, the fibroblast cells producing an infectious recombinant virus having a recombinant genome comprised of incorporated genetic material or keratinocytes and cell products produced by said fibroblast cells; b. maintaining said culture under conditions conducive to cell growth, whereby a sheet of keratinous tissue is formed on a surface of the vessel; and c. removing said sheet of keratinous tissue from the surface of the vessel.
Claim 5
Dependent Claim
21 words
A method of claim 2 in which the recombinant genome additionally comprises genetic material encoding at least one dominant selectable marker.
Claim 6
Dependent Claim
33 words
A method of claim 3 in which the sheet of keratinous tissue is contacted with a neutral protease under conditions sufficient for enzymatic detachment of the sheet from the surface of the vessel.
Claim 7
Dependent Claim
28 words
A method of claim 1 in which the incorporated genetic material encodes a hormone, an enzyme or a drug not normally expressed in keratinocyctes at biologically significant levels.
Claim 8
Dependent Claim
21 words
A method of claim 5 in which the recombinant genome additionally comprises genetic material encoding at least one dominant selectable marker.
Claim 9
Dependent Claim
26 words
A method of claim 6 in which the fibroblast cells are ATCC CRL8859 having incorporated therein the neo gene and the gene encoding human growth hormone.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-09-18 | 1993-03-20 | 1993-09-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-09-18 | 1997-03-20 | 1997-09-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-09-18 | 2001-03-20 | 2001-09-19 | $7700.00 | Window Closed / Lapsed |