US4650764A
Expired / Lapsed
US
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Helper cell
Assignee: Wisconsin Alumni Research Foundation
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Filing: 1984-03-26
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Granted: 1987-03-17
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Expires: 2004-03-26
Abstract
Abstract A helper cell for providing retrovirus protein which is required by a normally replication incompetent recombinant retrovirus gene sequence in order to replicate is disclosed. In one embodiment there is a eukaryotic host cell, a first retrovirus gene sequence in the cell which has a helper portion coding for a retrovirus protein and which is capable of expressing the retrovirus protein, and a defective portion which renders the gene sequence replication imcompetent. In this embodiment, there is also a second retrovirus gene sequence in the cell having a defective retrovirus port This invention was made with Government support under NIH Grant Nos. P01 CA 22443, P30 CA0 7175 and T32 CA0 9075 awarded by the Department of Health and Human Services. The Government has certain rights in this invention.
Inventors
- 👤 Howard M. Temin
- 👤 Shinichi Watanabe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 17:31
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/11011; C12N2740/11051; C12N2740/11052; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
176 words
A helper cell, comprising: a host cell; a first retrovirus helper gene sequence in the cell which has a helper portion coding for a retrovirus virion protein and which is capable of expressing the virion protein, and a defective encapsidation portion which renders the cell by itself unable to form the infectious virus which the first retrovirus helper gene sequence codes for; a second retrovirus helped gene sequence in the cell having a defective helped portion which would have rendered the cell unable to form the infectious virus which the second retrovirus helped gene sequence codes for if suitable virion protein had not been supplied from expression of the first retrovirus helper portion or from another source; and said second retrovirus helped gene sequence being a recombinant retrovirus sequence with a first part that is retrovirus and a second part that is a foreign gene protein coding part; whereby the virion protein expressed by the first helper portion can permit the second retrovirus helped gene sequence to form infectious recombinant virus coding for the foreign gene.
Claim 2
Dependent Claim
176 words
A helper cell, comprising: a host cell; a first retrovirus helper gene sequence in the cell which has a helper portion coding for a retrovirus virion protein and which is capable of expressing the virion protein, and a defective encapsidation portion which renders the cell by itself unable to form the infectious virus which the first retrovirus helper gene sequence codes for; a second retrovirus helped gene sequence in the cell having a defective helped portion which would have rendered the cell unable to form the infectious virus which the second retrovirus helped gene sequence codes for if suitable virion protein had not been supplied from expression of the first retrovirus helper portion or from another source; and said second retrovirus helped gene sequence being a recombinant retrovirus sequence with a first part that is retrovirus and a second part that is a foreign gene protein coding part; whereby the virion protein expressed by the first helper portion can permit the second retrovirus helped gene sequence to form infectious recombinant virus coding for the foreign gene.
Claim 3
Dependent Claim
12 words
The helper cell of claim 1, wherein the foreign gene is eukaryotic.
Claim 4
Dependent Claim
14 words
The helper cell of claim 2, wherein the foreign gene is a human gene.
Claim 5
Dependent Claim
51 words
The helper cell of claim 1, wherein the defective helper and helped portions are positioned along their respective gene sequences such that the first retrovirus helper gene sequence and the second retrovirus helped gene sequence will not recombine to form a single gene sequence capable of forming a complete infectious virus.
Claim 6
Dependent Claim
23 words
The helper cell of claim 1, wherein the first and second retrovirus gene sequences are not attached to each other in the cell.
References Cited (Backward Citations)
US4399216A
Cited
US4405712A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-03-16 | 1990-09-15 | 1991-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-03-16 | 1994-09-15 | 1995-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-03-16 | 1998-09-15 | 1999-03-17 | $7700.00 | Window Closed / Lapsed |