US5541313A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Single-stranded labelled oligonucleotides of preselected sequence
Assignee: Molecular Biosystems Inc
•
Filing: 1994-11-09
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Granted: 1996-07-30
•
Expires: 2014-11-09
Abstract
Abstract Substantially pure single-stranded oligonucleotides having a preselected sequence of not more than about 200 nucleotides, at least one of which is at a preselected position in the sequence and includes a base with a covalently attached linker arm containing or capable of binding at least one reporter group or solid support. A process for the chemical synthesis of the substantially pure single-stranded oligonucleotide and modified nucleosides useful in such synthesis are provided.
Inventors
- 👤 Jerry L. Ruth
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 15:27
CPC Classifications
C; C07; C07H; C07H21/00; C07H19/00; C07H19/02; C07H19/04; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
95 words
A composition consisting essentially of single-stranded oligonucleotides, wherein the sequence of each of said oligonucleotides is substantially identical to the sequence of each of the other oligonucleotides in said composition, wherein each of said oligonucleotides has a sequence of not more than approximately 200 nucleotides, wherein at least one of the nucleotide monomers from which each of said oligonucleotides is prepared has a linker arm attached to the base of said nucleotide, said nucleotide being substantially identically located in each of said oligonucleotides, and wherein said linker arm is further attached to a reporter group.
Claim 2
Dependent Claim
95 words
A composition consisting essentially of single-stranded oligonucleotides, wherein the sequence of each of said oligonucleotides is substantially identical to the sequence of each of the other oligonucleotides in said composition, wherein each of said oligonucleotides has a sequence of not more than approximately 200 nucleotides, wherein at least one of the nucleotide monomers from which each of said oligonucleotides is prepared has a linker arm attached to the base of said nucleotide, said nucleotide being substantially identically located in each of said oligonucleotides, and wherein said linker arm is further attached to a reporter group.
Claim 3
Dependent Claim
17 words
A composition according to claim 1 wherein said linker arm is attached at a sterically tolerant site.
Claim 4
Dependent Claim
23 words
A composition according to claim 1 wherein said base is a pyrimidine and said linker arm is attached to the C-5 position thereof.
Claim 5
Dependent Claim
23 words
A composition according to claim 1 wherein said base is a purine and said linker arm is attached to the C-8 position thereof.
Claim 6
Dependent Claim
20 words
A composition according to claim 1 wherein said identical sequence is between about 10 to about 40 nucleotides in length.
Claim 7
Dependent Claim
15 words
A composition according to claim 1 wherein said reporter group is a functional colorimetric group.
Claim 8
Dependent Claim
15 words
A composition according to claim 1 wherein said reporter group is a functional fluorescent group.
Claim 9
Dependent Claim
15 words
A composition according to claim 1 wherein said reporter group is a functional luminescent group.
Claim 10
Dependent Claim
15 words
A composition according to claim 1 wherein said reporter group is a functional radioactive group.
Claim 11
Dependent Claim
16 words
A composition according to claim 1 wherein said reporter group is a functional ligand recognition group.
Claim 12
Dependent Claim
12 words
A composition according to claim 1 wherein said reporter group is blocked.
Claim 13
Dependent Claim
92 words
A composition of single-stranded oligonucleotides wherein the sequence of each of the oligonucleotides in said composition is substantially identical to the sequence of each of the other oligonucleotides in said composition, wherein each of said oligonucleotides has a sequence of not more than approximately 200 nucleotides, wherein at least one of the nucleotides of each of said oligonucleotides has a linker arm attached to the base of said nucleotide, said nucleotide being substantially identically located in each of said oligonucleotides, and wherein said linker arm is further attached to a reporter group.
Claim 14
Dependent Claim
92 words
A composition of single-stranded oligonucleotides wherein the sequence of each of the oligonucleotides in said composition is substantially identical to the sequence of each of the other oligonucleotides in said composition, wherein each of said oligonucleotides has a sequence of not more than approximately 200 nucleotides, wherein at least one of the nucleotides of each of said oligonucleotides has a linker arm attached to the base of said nucleotide, said nucleotide being substantially identically located in each of said oligonucleotides, and wherein said linker arm is further attached to a reporter group.
Claim 15
Dependent Claim
16 words
The composition of claim 12 wherein said linker arm is attached at a sterically tolerant site.
Claim 16
Dependent Claim
22 words
The composition of claim 12 wherein said base is a pyrimidine and said linker arm is attached to the C-5 position thereof.
Claim 17
Dependent Claim
22 words
The composition of claim 12 wherein said base is a purine and said linker arm is attached to the C-8 position thereof.
Claim 18
Dependent Claim
19 words
The composition of claim 12 wherein said identical sequence is between about 10 to about 40 nucleotides in length.
Claim 19
Dependent Claim
15 words
A composition according to claim 12 wherein said reporter group is a functional colorimetric group.
Claim 20
Dependent Claim
15 words
A composition according to claim 12 wherein said reporter group is a functional fluorescent group.
Claim 21
Dependent Claim
15 words
A composition according to claim 12 wherein said reporter group is a functional luminescent group.
Claim 22
Dependent Claim
15 words
A composition according to claim 12 wherein said reporter group is a functional radioactive group.
Claim 23
Dependent Claim
16 words
A composition according to claim 12 wherein said reporter group is a functional ligand recognition group.
Claim 24
Dependent Claim
12 words
A composition according to claim 12 wherein said reporter group is blocked.
Claim 25
Dependent Claim
95 words
A composition consisting essentially of single-stranded oligonucleotides, wherein the sequence of each of said oligonucleotides is substantially identical to the sequence of each of the other oligonucleotides in said composition, wherein each of said oligonucleotides has a sequence of not more than approximately 200 nucleotides, wherein at least one of the nucleotide monomers from which each of said oligonucleotides is prepared has a linker arm attached to the base of said nucleotide, said nucleotide being substantially identically located in each of said oligonucleotides, and wherein said linker arm is further attached to a solid support.
Claim 26
Dependent Claim
95 words
A composition consisting essentially of single-stranded oligonucleotides, wherein the sequence of each of said oligonucleotides is substantially identical to the sequence of each of the other oligonucleotides in said composition, wherein each of said oligonucleotides has a sequence of not more than approximately 200 nucleotides, wherein at least one of the nucleotide monomers from which each of said oligonucleotides is prepared has a linker arm attached to the base of said nucleotide, said nucleotide being substantially identically located in each of said oligonucleotides, and wherein said linker arm is further attached to a solid support.
Claim 27
Dependent Claim
17 words
A composition according to claim 23 wherein said linker arm is attached at a sterically tolerant site.
Claim 28
Dependent Claim
23 words
A composition according to claim 23 wherein said base is a pyrimidine and said linker arm is attached to the C-5 position thereof.
Claim 29
Dependent Claim
23 words
A composition according to claim 23 wherein said base is a purine and said linker arm is attached to the C-8 position thereof.
Claim 30
Dependent Claim
20 words
A composition according to claim 23 wherein said identical sequence is between about 10 to about 40 nucleotides in length.
Claim 31
Dependent Claim
91 words
A composition of single-stranded oligonucleotides wherein the sequence of each of the oligonucleotides in said composition is substantially identical to the sequence of each of the other oligonucleotides in said composition, wherein each of said oligonucleotides has a sequence of not more than approximately 200 nucleotides, wherein at least one of the nucleotides of each said oligonucleotides has a linker arm attached to the base of said nucleotide, said nucleotide being substantially identically located in each of said oligonucleotides, and wherein said linker arm is further attached to a solid surface.
Claim 32
Dependent Claim
91 words
A composition of single-stranded oligonucleotides wherein the sequence of each of the oligonucleotides in said composition is substantially identical to the sequence of each of the other oligonucleotides in said composition, wherein each of said oligonucleotides has a sequence of not more than approximately 200 nucleotides, wherein at least one of the nucleotides of each said oligonucleotides has a linker arm attached to the base of said nucleotide, said nucleotide being substantially identically located in each of said oligonucleotides, and wherein said linker arm is further attached to a solid surface.
Claim 33
Dependent Claim
17 words
A composition according to claim 28 wherein said linker arm is attached at a sterically tolerant site.
Claim 34
Dependent Claim
23 words
A composition according to claim 28 wherein said base is a pyrimidine and said linker arm is attached to the C-5 position thereof.
Claim 35
Dependent Claim
23 words
A composition according to claim 28 wherein said base is a purine and said linker arm is attached to the C-8 position thereof.
Claim 36
Dependent Claim
20 words
A composition according to claim 28 wherein said identical sequence is between about 10 to about 40 nucleotides in length.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-07-30 | 2000-01-29 | 2000-07-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-07-30 | 2004-01-29 | 2004-07-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-07-30 | 2008-01-29 | 2008-07-30 | $7700.00 | Window Closed / Lapsed |