EP0198207A1
Expired / Lapsed
EP
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Specific iodination of nucleic acid
Assignee: Molecular Diagnostics Inc
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Filing: 1986-03-08
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Granted: —
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Expires: 2006-03-08
Abstract
Abstract A probe comprising a nucleic acid fragment covalently linked to an iodinatable residue. Such nucleic acid probes can be readily radiolabeled such as by prelabeling the nucleic acid with a nonradioactive iodinatable residue, and then reacting with radioactive iodine.
Inventors
- 👤 Nanibhushan Dattagupta
- 👤 William Dr. Knowles
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 23:39
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/534
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
13 words
A probe comprising a nucleic acid fragment covalently linked to an iodinatable residue.
Claim 2
Dependent Claim
13 words
A probe comprising a nucleic acid fragment covalently linked to an iodinatable residue.
Claim 3
Dependent Claim
21 words
A probe according to claim 1, wherein the iodinatable residue is a hydroxyphenyl radical, an indole radical or an imidazole radical.
Claim 4
Dependent Claim
16 words
A probe according to any of Claims 1 and 2, wherein the linkage includes a -CON-radical.
Claim 5
Dependent Claim
38 words
A probe according to any of Claims 1 to 3, of the formula Nucleic Acid -R -NH -CO -X, wherein R is an aliphatic hydrocarbon radical of up to 11 carbon atoms and X is an iodinatable residue.
Claim 6
Dependent Claim
9 words
A probe according to claim 4, wherein X is
Claim 7
Dependent Claim
14 words
A probe according to claim 4, wherein R has 3 to 6 carbon atoms.
Claim 8
Dependent Claim
26 words
A probe according to claim 4, wherein R is attached to the sugar of the nucleic acid or to the heterocyclic moiety of the nucleic acid.
Claim 9
Dependent Claim
54 words
A process for producing an iodinated probe of the formula Nucleic Acid wherein X is an iodinatable residue, R is an aliphatic hydrocarbon radical of up to 11 carbon atoms and n = 1 to 4, which comprises reacting a probe of the formula Nucleic Acid -R -NH -CO -X with an iodinating agent.
Claim 10
Dependent Claim
54 words
A process for producing an iodinated probe of the formula Nucleic Acid wherein X is an iodinatable residue, R is an aliphatic hydrocarbon radical of up to 11 carbon atoms and n = 1 to 4, which comprises reacting a probe of the formula Nucleic Acid -R -NH -CO -X with an iodinating agent.
Claim 11
Dependent Claim
34 words
A process for separating an iodinated probe produced by the process of claim 8, which comprises passing the reaction mass through a chromatographic column and separating a substantially pure fraction comprising the iodinated probe.
Claim 12
Dependent Claim
38 words
Use of an iodinated probe of the formula Nucleic Acid wherein X ia an iodinatable residue, R is an aliphatic Hydrocaron radical of up to 11 carbon atoms and n = 1 to 4, in nucleic acid hybridization.
Claim 13
Dependent Claim
38 words
Use of an iodinated probe of the formula Nucleic Acid wherein X ia an iodinatable residue, R is an aliphatic Hydrocaron radical of up to 11 carbon atoms and n = 1 to 4, in nucleic acid hybridization.
References Cited (Backward Citations)
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